Abstract: The present invention provides methods and compositions for sustained methane production from atmospheric CO2 and solar energy from the sun. In general the methods involve culturing cyanobacteria in a first culture vessel and collecting and diverting the photosynthesis products, including glucose or acetic acid, to a second culture vessel including methanogenic bacteria. The photosynthesis products are then used as nutrients by the methanogenic bacteria in the second culture vessel in the production of methane. The methane produced is subsequently collected and used as a clean energy source. The invention also features compositions, including genetically modified cyanobacteria and systems for use in the methods of the invention.

Abstract: The present invention provides methods and compositions for sustained methane production from atmospheric CO2 and solar energy from the sun. In general the methods involve culturing cyanobacteria in a first culture vessel and collecting and diverting the photosynthesis products, including glucose or acetic acid, to a second culture vessel including methanogenic bacteria. The photosynthesis products are then used as nutrients by the methanogenic bacteria in the second culture vessel in the production of methane. The methane produced is subsequently collected and used as a clean energy source. The invention also features compositions, including genetically modified cyanobacteria and systems for use in the methods of the invention.

Abstract: The present invention provides methods and compositions for sustained methane production from atmospheric CO2 and solar energy from the sun. In general the methods involve culturing cyanobacteria in a first culture vessel and collecting and diverting the photosynthesis products, including glucose or acetic acid, to a second culture vessel including methanogenic bacteria. The photosynthesis products are then used as nutrients by the methanogenic bacteria in the second culture vessel in the production of methane. The methane produced is subsequently collected and used as a clean energy source. The invention also features compositions, including genetically modified cyanobacteria and systems for use in the methods of the invention.

Abstract: Provided are caged NADP and NAD compounds. The invention includes two types of caged NAD and NADP (NAD/P) analogs, each type having a different caging group binding at a different site on the molecule: Phosphate-caged DMNPE-NAD/P and nicotinamide-caged CNB-NAD/P. Both types of caged molecules are designed to be inactive in their caged configurations and activated upon photolysis of the caging group. In some applications, the different types of caged compounds may exhibit biochemically distinct activities. For example, "catalytically caged" NAD/P compounds according to the present invention may bind to an enzyme but not allow turnover prior to photolytic activation. This type of compound has the advantage, when used as a photolytic trigger for time-resolved studies, that it is bound to the enzyme active site prior to photolysis so that no additional diffusion or binding events are necessary to form the Michaelis complex.