Abstract: Homogeneous fluorescence polarization (anisotropy) assays for detecting and quantifying metal ions in solution, based the metal-dependent binding of a fluorescent ligand to an unlabeled macromolecule, or the binding of a metal ion to a fluorescent labeled macromolecule. The metal-dependent binding of a fluorescent ligand to an unlabeled macromolecule (metallo-macromolecule) effects a measurable change in anisotropy as will the binding of metal ions to a fluorescent labeled macromolecule. Binding of the fluorescent ligand to the unlabeled macromolecule is metal dependent with the change in anisotropy being proportional to the concentration of bound metal ions. No binding of the fluorescent ligand to the macromolecule occurs in the absence of metal ions. Conversely, if the fluorescent label is first conjugated to a metallo-macromolecule and the metallo-macromolecule is subsequently stripped of its metal ion, it may then be used to transduce the binding of metal ions.

Abstract: A mobile funds transaction device for transferring funds between one bank account or credit facility and another bank account or credit facility, the device having: a card reader device; a PINpad device with an encryption system; a modem device; a communication interface device connected with the modem device; an output report device; a wireless communication device; and a central processing device (CPU) to which are connected the above-listed devices. The CPU is programmed to store merchant identifying data of the owner/leasee of the device and to activate the PINpad device so a user can enter confidential identifying information to the CPU.