Abstract: Provided herein are compositions and methods related to CMV gB proteins in which amino acid substitutions have been made to disrupt the postfusion state and/or stabilize the prefusion state.

Abstract: Nanoparticles and vaccines containing these nanoparticles that elicit an immune response against hemagglutinin (HA) proteins from a broad range of influenza viruses are provided. The nanoparticles are made from self-assembling proteins, at least one of which is joined to an immunogenic portion of an influenza HA protein. This immunogenic portion is displayed on the nanoparticle surface, making the nanoparticles useful for vaccinating subjects against influenza virus.

Abstract: Provided are protein nanostructures that display Neisseria meningitidis factor H binding protein (fHBP). The protein nanostructure may be a two-component icosahedral nanostructure. Further provided are vaccine compositions, methods of manufacturing, and methods of use, e.g., immunizing a subject to generate a protective immune response to Neisseria meningitidis.

Abstract: Polypeptides having an amino acid sequence at least 50% identical to, and identical at least at one identified interface position, to the amino acid sequence selected from the group consisting of SEQ ID NO: 1-44, and polypeptides having an amino acid sequence at least 50% identical to the amino acid sequence selected from the group consisting of SEQ ID NO: 45-58, are provided, as well as fusion proteins thereof, nanoparticles thereof, and methods for treating or limiting development of an infection.

Abstract: Provided herein are recombinant polypeptides comprising an engineered ectodomain of a viral protein from enveloped viruses. Also provided herein are two-component protein nanostructures and compositions for use in vaccinating, generating an immune response, or treating or preventing a viral infection.

Abstract: Disclosed herein are polypeptides that include (a) a heptad motif domain comprising an amino acid sequence according to the genus (I-X1-X2-I-X3-X4-X5)n, wherein X1, X2, X3, X4, and X5 may independently be any amino acid other than proline, and wherein n can be 1-30; and (b) a second domain selected from the group consisting of (i) a polypeptide antigen, and (ii) a polypeptide component of a nanoparticle, nanoparticles including such polypeptides, and uses thereof.

Abstract: Provided herein are recombinant polypeptides comprising an engineered ectodomain of a Respiratory Syncytial Virus (RSV) fusion (F) protein, wherein the ectodomain comprises an engineered C-terminal alpha-helical segment and/or amino acid substitutions that stabilize the F protein in a prefusion conformation. The disclosure also provides a two-component protein nanostructure comprising first trimeric component and second pentameric component. Provided herein are a composition for use in vaccinating, generating an immune response, or treating or preventing RSV disease.

Abstract: Provided herein are compositions and methods comprising mutated coronavirus “S” spike proteins or receptor binding domains thereof that have an increased expression level, yield and stability compared to its corresponding native or wild-type coronavirus spike protein under the same expression, culture or storage conditions. These mutated spike proteins can be used for generating a protein-based vaccine against one or more coronaviruses.

Abstract: The present disclosure provides systems and methods that generating health diagnostic information from an audio recording. A computing system can include a machine-learned health model comprising that includes a sound model trained to receive data descriptive of a patient audio recording and output sound description data. The computing system can include a diagnostic model trained to receive the sound description data and output a diagnostic score. The computing system can include at least one tangible, non-transitory computer-readable medium that stores instructions that, when executed, cause the processor to perform operations. The operations can include obtaining the patient audio recording; inputting data descriptive of the patient audio recording into the sound model; receiving, as an output of the sound model, the sound description data; inputting the sound description data into the diagnostic model; and receiving, as an output of the diagnostic model, the diagnostic score.

Abstract: Provided herein are compositions and methods comprising mutated coronavirus “S” spike proteins or receptor binding domains thereof that have an increased expression level, yield and stability compared to its corresponding native or wild-type coronavirus spike protein under the same expression, culture or storage conditions. These mutated spike proteins can be used for generating a protein-based vaccine against one or more coronaviruses.

Abstract: The present disclosure provides systems and methods that generating health diagnostic information from an audio recording. A computing system can include a machine-learned health model comprising that includes a sound model trained to receive data descriptive of a patient audio recording and output sound description data. The computing system can include a diagnostic model trained to receive the sound description data and output a diagnostic score. The computing system can include at least one tangible, non-transitory computer-readable medium that stores instructions that, when executed, cause the processor to perform operations. The operations can include obtaining the patient audio recording; inputting data descriptive of the patient audio recording into the sound model; receiving, as an output of the sound model, the sound description data; inputting the sound description data into the diagnostic model; and receiving, as an output of the diagnostic model, the diagnostic score.

Abstract: The present disclosure provides nanostructures and nanostructure-based vaccines. Some nanostructures of the present disclosure display antigens capable of eliciting immune responses to infectious agents such as bacteria, viruses, and pathogens. Some vaccines of the present disclosure are useful for preventing or decreasing the severity of infection with an infectious agent, including. for example and without limitation, lyme disease, pertussis, herpes virus, orthomyxovirus, paramyxovirus, pneumovirus, filovirus. flavivirus, reovirus, retrovirus, meningococcus, or malaria. The antigens may be attached to the core of the nanostructure either non-covalently or covalently, including as a fusion protein or by other means disclosed herein. Multimeric antigens may optionally be displayed along a symmetry axis of the nanostructure. Also provided are proteins and nucleic acid molecules encoding such proteins. vaccine compositions, and methods of administration.

Abstract: Nanostructures and nanostructure-based vaccines that display antigens capable of eliciting immune responses to infectious agents such as bacteria, viruses, and pathogens are provided. Some vaccines are useful for preventing or decreasing the severity of infection with an infectious agent, including, for example and without limitation, lyme disease, pertussis, herpes virus, orthomyxovirus, paramyxovirus, pneumovirus, filovirus, flavivirus, reovirus, retrovirus, meningococcus, or malaria. The antigens may be attached to the core of the nanostructure either non-covalently or covalently, including as a fusion protein or by other means. Multimeric antigens may optionally be displayed along a symmetry axis of the nanostructure. Also provided are proteins and nucleic acid molecules encoding such proteins, vaccine compositions, and methods of administration.

Abstract: Synthetic nanostructures, polypeptides that are useful, for example, in making synthetic nanostructures, and methods for using such synthetic nanostructures are disclosed herein.

Abstract: The disclosure provides non-naturally occurring mutant neuraminidase (NA) polypeptides that improve expression and or modifies the open/closed tetrameric conformational state of the NA polypeptide, and uses thereof.

Abstract: Systems and methods of controlling a docking station comprise establishing, by the wireless access point, a USB connection tunnelled over a local area wireless network connection with the mobile device, receiving, by the wireless access point over the USB connection, a request for an Internet Protocol (IP) or a Media Access Control (MAC) network address of the display controller, transmitting, by the wireless access point in response to the request, the IP or the MAC network address of the display controller to the mobile device over the USB connection, and receiving, by the wireless access point, the at least video data addressed to the IP or the MAC network address of the display controller and switching the at least video data addressed to the IP or the MAC network address of the display controller directly to the display controller without passing via the USB controller.

Abstract: Synthetic nanostructures, polypeptides that are useful, for example, in making synthetic nanostructures, and methods for using such synthetic nanostructures are disclosed herein.

Abstract: A computing system can include an embedding model and a clustering model. The computing system input each of the plurality of inputs into the embedding model and receiving respective embeddings for the plurality of inputs as outputs of the embedding model. The computing system can input the respective embeddings for the plurality of inputs into the clustering model and receiving respective cluster assignments for the plurality of inputs as outputs of the clustering model. The computing system can evaluate a clustering loss function that evaluates a first average, across the plurality of inputs, of a respective first entropy of each respective probability distribution; and a second entropy of a second average of the probability distributions for the plurality of inputs. The computing system can modify parameter(s) of one or both of the clustering model and the embedding model based on the clustering loss function.

Abstract: Provided herein are compositions and methods comprising mutated coronavirus “S” spike proteins or receptor binding domains thereof that have an increased expression level, yield and stability compared to its corresponding native or wild-type coronavirus spike protein under the same expression, culture or storage conditions. These mutated spike proteins can be used for generating a protein-based vaccine against one or more coronaviruses.

Abstract: Methods are provided for generating training triplets that can be used to train multidimensional embeddings to represent the semantic content of non-speech sounds present in a corpus of audio recordings. These training triplets can be used with a triplet loss function to train the multidimensional embeddings such that the embeddings can be used to cluster the contents of a corpus of audio recordings, to facilitate a query-by-example lookup from the corpus, to allow a small number of manually-labeled audio recordings to be generalized, or to facilitate some other audio classification task. The triplet sampling methods may be used individually or collectively, and each represent a respective heuristic about the semantic structure of audio recordings.