Abstract: An injection shield for protecting a person's hand during the injection of a needle into a rodent's tail. The shield comprises a frame which includes a coupler for a rodent restrainer, and contains an opening for a rodent's tail. The frame includes an injection surface on an injection side of the frame. The coupler is adapted to receive and releasably secure the restrainer in an orientation that permits the tail to be placed on the injection surface of the frame. The opening is dimensioned to allow the tail to pass through the frame, from the injection side to a shielded side. The opening is a sufficient distance from the coupler to allow the tail to extend through the opening and be grasped by the person's hand or otherwise secured. A method of protecting a person's hand during the injection of a needle into a rodent's tail is also contemplated by the present invention.