Abstract: Soybean plants with one or more non-transgenic human-induced mutations of the phytate transport genes, LPA-3 and LPA-19, and the agglutinin gene, LEC1, are disclosed. Soybean plants and seeds having reduced levels of phytate and/or lectin as a result of such non-transgenic human-induced mutations are provided. Meal prepared from soybean seeds provided herein is useful, for example, as a source of food or feed for poultry, human, swine, or fish, wherein the food or feed has reduced levels of antinutrient factors, including phytate and lectin.

Abstract: Soybean plants with one or more non-transgenic human-induced mutations of the phytate transport genes, LPA-3 and LPA-19, and the agglutinin gene, LEC1, are disclosed. Soybean plants and seeds having reduced levels of phytate and/or lectin as a result of such non-transgenic human-induced mutations are provided. Meal prepared from soybean seeds provided herein is useful, for example, as a source of food or feed for poultry, human, swine, or fish, wherein the food or feed has reduced levels of antinutrient factors, including phytate and lectin.

Abstract: The present invention relates to compositions and methods for preparing poly-unsaturated long chain fatty acids in plants, plant parts and plant cells, such as leaves, roots, fruits and seeds. Nucleic acid sequences and constructs encoding PKS-like genes required for the poly-unsaturated long chain fatty acid production, including the genes responsible for eicosapentenoic acid production of Shewanella putrefaciens and novel genes associated with the production of docosahexenoic acid in Vibrio marinus are used to generate transgenic plants, plant parts and cells which contain and express one or more transgenes encoding one or more of the PKS-like genes associated with such long chain polyunsaturated fatty acid production. Expression of the PKS-like genes in the plant system permits the large scale production of poly-unsaturated long chain fatty acids such as eicosapentenoic acid and docosahexenoic acid for modification of the fatty acid profile of plants, plant parts and tissues.

Abstract: Non-naturally occurring soybean plants and seeds having reduced isoflavones are provided. A series of independent non-transgenic human-induced mutations found in one or more isoflavone synthase genes of soybean; soybean plants having these mutations in one or more isoflavone synthase genes; and a method of creating and finding similar and/or additional mutations of the isoflavone synthase gene by screening pooled and/or individual DNA of soybean plants. The results are soybean plants and seeds having reduced isoflavones.

Abstract: Non-naturally occurring soybean plants and seeds having reduced isoflavones are provided. A series of independent non-transgenic human-induced mutations found in one or more isoflavone synthase genes of soybean; soybean plants having these mutations in one or more isoflavone synthase genes; and a method of creating and finding similar and/or additional mutations of the isoflavone synthase gene by screening pooled and/or individual DNA of soybean plants. The results are soybean plants and seeds having reduced isoflavones.

Abstract: The present invention relates to compositions and methods for preparing poly-unsaturated long chain fatty acids in plants, plant parts and plant cells, such as leaves, roots, fruits and seeds. Nucleic acid sequences and constructs encoding PKS-like genes required for the poly-unsaturated long chain fatty acid production, including the genes responsible for eicosapentenoic acid production of Shewanella putrefaciens and novel genes associated with the production of docosahexenoic acid in Vibrio marinus are used to generate transgenic plants, plant parts and cells which contain and express one or more transgenes encoding one or more of the PKS-like genes associated with such long chain polyunsaturated fatty acid production. Expression of the PKS-like genes in the plant system permits the large scale production of poly-unsaturated long chain fatty acids such as eicosapentenoic acid and docosahexenoic acid for modification of the fatty acid profile of plants, plant parts and tissues.

Abstract: The present invention relates to compositions and methods for preparing poly-unsaturated long chain fatty acids in plants, plant parts and plant cells, such as leaves, roots, fruits and seeds. Nucleic acid sequences and constructs encoding PKS-like genes required for the poly-unsaturated long chain fatty acid production, including the genes responsible for eicosapentenoic acid production of Shewanella putrefaciens and novel genes associated with the production of docosahexenoic acid in Vibrio marinus are used to generate transgenic plants, plant parts and cells which contain and express one or more transgenes encoding one or more of the PKS-like genes associated with such long chain polyunsaturated fatty acid production. Expression of the PKS-like genes in the plant system permits the large scale production of poly-unsaturated long chain fatty acids such as eicosapentenoic acid and docosahexenoic acid for modification of the fatty acid profile of plants, plant parts and tissues.

Abstract: The present invention relates to compositions and methods for preparing poly-unsaturated long chain fatty acids in plants, plant parts and plant cells, such as leaves, roots, fruits and seeds. Nucleic acid sequences and constructs encoding PKS-like genes required for the poly-unsaturated long chain fatty acid production, including the genes responsible for eicosapentenoic acid production of Shewanella putrefaciens and novel genes associated with the production of docosahexenoic acid in Vibrio marinus are used to generate transgenic plants, plant parts and cells which contain and express one or more transgenes encoding one or more of the PKS-like genes associated with such long chain polyunsaturated fatty acid production. Expression of the PKS-like genes in the plant system permits the large scale production of poly-unsaturated long chain fatty acids such as eicosapentenoic acid and docosahexenoic acid for modification of the fatty acid profile of plants, plant parts and tissues.

Abstract: The present invention relates to compositions and methods for preparing poly-unsaturated long chain fatty acids in plants, plant parts and plant cells, such as leaves, roots, fruits and seeds. Nucleic acid sequences and constructs encoding PKS-like genes required for the poly-unsaturated long chain fatty acid production, including the genes responsible for eicosapentenoic acid production of Shewanella putrefaciens and novel genes associated with the production of docosahexenoic acid in Vibrio marinus are used to generate transgenic plants, plant parts and cells which contain and express one or more transgenes encoding one or more of the PKS-like genes associated with such long chain polyunsaturated fatty acid production. Expression of the PKS-like genes in the plant system permits the large scale production of poly-unsaturated long chain fatty acids such as eicosapentenoic acid and docosahexenoic acid for modification of the fatty acid profile of plants, plant parts and tissues.

Abstract: Rapeseed in plants producing rapeseed which have a yellow-seed coat are provided. The yellow-seed coat is controlled by a single locus mutation. Plants from the rapeseed are useful for transferring the trait into elite lines of canola.

Abstract: Rapeseed in plants producing rapeseed which have a yellow-seed coat are provided. The yellow-seed coat is controlled by a single locus mutation. Plants from the rapeseed are useful for transferring the trait into elite lines of canola.

Abstract: Rapeseed in plants producing rapeseed which have a yellow-seed coat are provided. The yellow-seed coat is controlled by a single locus mutation. Plants from the rapeseed are useful for transferring the trait into elite lines of canola.

Abstract: The present invention relates to compositions and methods for preparing poly-unsaturated long chain fatty acids in plants, plant parts and plant cells, such as leaves, roots, fruits and seeds. Nucleic acid sequences and constructs encoding PKS-like genes required for the poly-unsaturated long chain fatty acid production, including the genes responsible for eicosapentenoic acid production of Shewanella putrefaciens and novel genes associated with the production of docosahexenoic acid in Vibrio marinus are used to generate transgenic plants, plant parts and cells which contain and express one or more transgenes encoding one or more of the PKS-like genes associated with such long chain polyunsaturated fatty acid production. Expression of the PKS-like genes in the plant system permits the large scale production of poly-unsaturated long chain fatty acids such as eicosapentenoic acid and docosahexenoic acid for modification of the fatty acid profile of plants, plant parts and tissues.

Abstract: Regulatory regions from genes expressed during a particular developmental stage or in a specific tissue are identified employing cDNA screening. The resulting regulatory regions are manipulated for use with foreign sequences for introduction into plant cells to provide transformed plants having phenotypic property which can be modulated. The invention is exemplified with light, seed and fruit-specific promoters.

Abstract: Novel methods and compositions are provided for regenerating untransformed or transgenic leguminous plants from thin layer explants of immature embryonic cotyledons. Transgenic plants are preferably obtained by bombarding the thin layer explants at high velocity with DNA expression cassettes adsorbed to tungsten particles.

Abstract: An array of micro-needles extending from a support substrate and having tip portions shaped and dimensioned to (1) carry a biological substance and (2) pierce the surface of a target tissue and penetrate target cells within the tissue where the biological substance transfers from the tip portions and deposits within the target cells.

Abstract: Microinjection techniques for plant protoplasts utilize a holding pipette for immobilizing the protoplast while an injection pipette is utilized to inject the macromolecule. In order to manipulate the protoplasts without damage, the protoplasts are cultured for from about 1 to 5 days before the injection is performed to allow for partial regeneration of the cell wall. It was found that injection through the partially regenerated cell wall could still be accomplished and particular compartments of the cell could be targeted. The methods are particularly useful for transformation of plant protoplasts with exogenous genes.