Abstract: An aerospace vehicle system, preferably including one or more capsules, and optionally including one or more propulsion modules and/or safety modules. A capsule, preferably including a pressure vessel including a structural frame and one or more windows, and optionally including a cabin. In alternate embodiments, the system can additionally or alternatively function as a terrestrial vehicle, a watercraft, and/or any other suitable vehicle. A method of operation, preferably including operating the aerospace vehicle system in flight, while maintaining a pressure differential between the pressure vessel interior and an environment surrounding the system.

Abstract: Methods, non-transitory computer readable media, and surgical computing devices are disclosed herein for creating a three-dimensional (3D) model based on a plurality of received two-dimensional (2D) medical images containing patient anatomy and a tracking fiducial. Once the 2D images are received, a determination is made regarding any potential processing steps required to put the images into a standard view. Once the images are processed, a user may modify or adjust various factors. Using the size and orientation of the tracking fiducial a 3D virtual model is created based on a repository of known patient data, such as a bone atlas. The 3D virtual model can then be output to a display device and optionally used to facilitate a surgical procedure. The 3D virtual model of patient anatomy can advantageously be generated more quickly and using fewer resources with this technology.

Abstract: A nucleotide sequence encoding the RNA genome of a lentiviral vector, wherein the major splice donor site in the RNA genome of the lentiviral vector is inactivated, and wherein the cryptic splice donor site 3? to the major splice donor site is inactivated.

Abstract: The present disclosure provides a surgical navigation system that utilizes multimodal tracking along with low profile/small diameter bone pins to fix FBG sensors to a patient. With some embodiments, a multi-core fiber optic cable having both an infrared (IR) tracking sensor disposed at a known location in the multi-core fiber optic cable and FBGs. The FBGs can be used to locate the tip of the cable relative to the IR marker, where the tip of the cable is embedded in a bone, the location of the done can be determined.

Abstract: A surgical system and method for markerless intraoperative navigation is provided. The system can includes a structured light system that can be utilized to intraoperatively sense three-dimensional surface geometry. The computer system is configured to segment a depth image to obtain surface geometry data of an anatomical structure embodied within the depth image, register the surface geometry data of the anatomical structure to a model, and update the surgical plan according to the registered surface geometry data. The surgical system is an endoscopic surgical system.

Abstract: Methods and system for characterizing ligament properties using elastography are disclosed. An ultrasound system capable of performing shear wave elasticity imaging and/or supersonic shear imaging may retrieve one or more images from a proposed surgical site. The one or more images may be provided to a surgical planning system that identifies one or more properties of ligaments proximate to the surgical site. Musculoskeletal simulations may be performed using the identified properties to preoperatively identify a surgical plan. Preoperative identification of a surgical plan may enable a surgeon to select from more fine-tuning options for a joint replacement than conventional systems.

Abstract: The present invention provides a novel method for detecting replication competent virus in a test sample. The method comprises culturing and diluting a plurality of individual cell culture aliquots comprising virus-permissive cells and a portion of the test sample, followed by testing for the presence of replication competent virus. The method may be used in parallel with a positive control, which is also provided herein.

Abstract: Methods and systems of augmenting an implant intraoperatively and preparing a cone for revision surgical procedure are disclosed. A system includes a cutting device, a tracking and navigation system and a cutting system in operable communication with the cutting device and the tracking and navigation system. The cutting device includes a communication system, a cutting element, and a plurality of optical trackers. The tracking and navigation system is configured to detect a location of optical trackers. The control system is configured to cause the tracking and navigation system to detect the location of the cutting device, determine a revised shape for an implant cavity, cause the cutting device to cut the implant cavity to the revised shape, select a shape for a cone to be placed in the revised implant cavity, and machine the cone to the selected shape.

Abstract: Methods and system for characterizing ligament properties using elastography are disclosed. An ultrasound system capable of performing shear wave elasticity imaging and/or supersonic shear imaging may retrieve one or more images from a proposed surgical site. The one or more images may be provided to a surgical planning system that identifies one or more properties of ligaments proximate to the surgical site. Musculoskeletal simulations may be performed using the identified properties to preoperatively identify a surgical plan. Preoperative identification of a surgical plan may enable a surgeon to select from more fine-tuning options for a joint replacement than conventional systems.

Abstract: The present invention provides novel methods for producing a viral vector. Corresponding viral vector production systems and uses are also provided.

Abstract: A cell for producing retroviral vectors comprising nucleic acid sequences encoding: i) gag-pol; ii) env; iii) the RNA genome of the retroviral vector; and iv) optionally rev, or a functional substitute thereof, wherein at least two nucleic acid sequences are located at the same genetic locus; and wherein the at least two nucleic acid sequences are in reverse and/or alternating orientations.

Abstract: A tensioner tool for assessing joint laxity is disclosed. The tensioner tool comprises a pair of pivotally coupled arms, each arm comprising a proximal handle portion, a distal portion, and an insertion tip selectively coupled to the distal portion. The pair of arms pivot between a compressed configuration for insertion within the joint and an expanded configuration for distraction of the joint by applying a force to the handles, thus spreading the insertion tips. The tensioner tool also comprises a force sensor configured to measure the force applied to the handle portion and a positional sensor configured to measure a separation distance between the pair of arms. The tensioner tool also comprises a processor configured to calculate a distraction force at the insertion tips based on the measured force and to calculate a tip distance between the insertion tips based on the measured separation distance.

Abstract: Methods and systems of augmenting an implant intraoperatively and preparing a cone for revision surgical procedure are disclosed. A system includes a cutting device, a tracking and navigation system and a cutting system in operable communication with the cutting device and the tracking and navigation system. The cutting device includes a communication system, a cutting element, and a plurality of optical trackers. The tracking and navigation system is configured to detect a location of optical trackers. The control system is configured to cause the tracking and navigation system to detect the location of the cutting device, determine a revised shape for an implant cavity, cause the cutting device to cut the implant cavity to the revised shape, select a shape for a cone to be placed in the revised implant cavity, and machine the cone to the selected shape.

Abstract: Patient-specific guides for the Latarjet procedure, as well as surgical systems and methods of performing the Latarjet procedure to treat glenohumeral instability using such patient-specific guides are disclosed. A patient-specific coracoid guide and a patient-specific glenoid guide may be configured based on preoperatively generated three-dimensional models of the patient's shoulder anatomy. Guides may be configured for coracoid graft preparation and glenoid decortication. The coracoid graft may be placed in the desired position based on three-dimensional (3D) preoperative planning.

Abstract: Disclosed herein is a system and a method for registering a surgical site to a surgical navigation system when the surgical site contains an implant for revision joint replacement surgery. The method creates a depth map of the surgical site and provides a possible identification of the existing implant using surface matching algorithms. The model of the implant is iteratively oriented with respect to the to the depth map until an optimal fit is achieved. Once the implant has been identified and localized, the registered landmarks are used in robotic-assisted surgery.

Abstract: The present invention relates to a nucleic acid sequence comprising a nucleotide of interest and a tryptophan RNA-binding attenuation protein (TRAP) binding site, and optionally a Kozak sequence, wherein said TRAP binding site overlaps the Kozak sequence and/or the ATG start codon of the nucleotide of interest. The present invention further relates to a nucleic acid sequence comprising a nucleotide of interest and a Kozak sequence, wherein said Kozak sequence comprises a portion of a tryptophan RNA-binding attenuation protein (TRAP) binding site. The present invention further relates to a nucleic acid sequence comprising a nucleotide of interest and TRAP binding site wherein the TRAP binding site comprises a portion of the start codon ATG of said nucleotide of interest or wherein the ATG start codon comprises a portion of the TRAP binding site.

Abstract: Systems and methods for determining a care plan for a patient are disclosed. Data from a one or more databases are received or retrieved and used to determine a preferred care plan used to perform a surgical procedure or otherwise treat a patient. The data may include data pertaining to a patient, a healthcare professional, a healthcare facility, an implant, economic data, simulation data, imaging data, and/or the like. The data may be used to determine a plan that provides a positive outcome and patient satisfaction. The data may be updated over time or in real time to improve or refine the determination of care plans for the current patient or other patients.

Abstract: Patient-specific guides for the Latarjet procedure, as well as surgical systems and methods of performing the Latarjet procedure to treat glenohumeral instability using such patient-specific guides are disclosed. A patient-specific coracoid guide and a patient-specific glenoid guide may be configured based on preoperatively generated three-dimensional models of the patient's shoulder anatomy. Guides may be configured for coracoid graft preparation and glenoid decortication. The coracoid graft may be placed in the desired position based on three-dimensional (3D) preoperative planning.

Abstract: An automated spatial frame is disclosed. The spatial frame may include a master controller unit arranged and configured as a centralized controller for exchanging data with a remote computing system, exchanging data with a plurality of automated struts, and delivering power to the automated struts. Thus arranged, the master-controller unit may be configured as a fully integrated, rechargeable power supply/controller unit for powering and controlling the automated struts. In one embodiment, the master-controller unit is coupled to an external surface of a platform. The platform acting as a conduit for coupling the master-controller unit to the automated struts. As such, at least one of the platforms provides integrated connectivity to the automated struts. In one embodiment, the struts may be wireless automated strut including a motor, a power source, and a wireless communications module for communicating with an external computing system.

Abstract: A modified U1 snRNA, wherein said modified U1 snRNA has been modified to bind to a nucleotide sequence within the packaging region of a lentiviral vector genome sequence.