Patents by Inventor Daniel G. ISOM

Daniel G. ISOM has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 9499580
    Abstract: Internal polar and ionizable groups are essential for enzymatic catalysis, proton transport, redox reactions, and many other functional properties of proteins. To engineer novel enzymes or to modify the function of existing ones, and to build switches that can be used to modify the stability of proteins in response to changes in pH, it is necessary to introduce polar or ionizable groups or to modify the properties of existing ones. However, internal polar and ionizable groups usually destabilize proteins. The disclosure provides new methods that allow the introduction of polar and ionizable groups into the interior of proteins, as well as new methods for improving the accuracy of pKa of an internal amino acid of a protein, and methods for mapping the folding free energy landscape of a protein.
    Type: Grant
    Filed: December 2, 2010
    Date of Patent: November 22, 2016
    Assignee: THE JOHNS HOPKINS UNIVERSITY
    Inventors: Bertrand E. Garcia-Moreno, Daniel G. Isom
  • Publication number: 20120258518
    Abstract: Internal polar and ionizable groups are essential for enzymatic catalysis, proton transport, redox reactions, and many other functional properties of proteins. To engineer novel enzymes or to modify the function of existing ones, and to build switches that can be used to modify the stability of proteins in response to changes in pH, it is necessary to introduce polar or ionizable groups or to modify the properties of existing ones. However, internal polar and ionizable groups usually destabilize proteins. The disclosure provides new methods that allow the introduction of polar and ionizable groups into the interior of proteins, as well as new methods for improving the accuracy of pKa of an internal amino acid of a protein, and methods for mapping the folding free energy landscape of a protein.
    Type: Application
    Filed: December 2, 2010
    Publication date: October 11, 2012
    Applicant: THE JOHNS HOPKINS UNIVERSITY
    Inventors: Bertrand E. Garcia-Moreno, Daniel G. Isom
  • Publication number: 20110171737
    Abstract: Methods of optimizing mRNA sequences for expression in host cells are provided. Methods of determining the stability of a protein are also provided. Methods of determining the affinity of a ligand for a protein are also provided.
    Type: Application
    Filed: November 24, 2010
    Publication date: July 14, 2011
    Inventors: Homme W. HELLINGA, Malin J. ALLERT, Daniel G. ISOM, Terrence G. OAS