Abstract: Circular RNA and methods and constructs for engineering circular RNA are disclosed. In some embodiments, the circular RNA includes the following elements arranged in the following sequence: a) a 3? Group I self-splicing intron fragment, b) an internal ribosome entry site (IRES), c) a protein coding region or noncoding region, and d) a 5? Group I self-splicing intron fragment.

Abstract: Disclosed are methods and constructs for engineering circular RNA Disclosed is a vector for making circular RNA, said vector comprising the following elements operably connected to each other and arranged in the following sequence: a) a 5? homology arm, b) a 3? group I intron fragment containing a 3? splice site dinucleotide, c) an optional 5? spacer sequence, d) a protein coding or noncoding region, e) an optional 3? spacer sequence, f) a 5? Group I intron fragment containing a 5? splice site dinucleotide, and g) a 3? homology arm. This vector allows production of a circular RNA that is translatable or biologically active inside eukaryotic cells. In one embodiment, the vector can comprise the 5? spacer sequence, but not the 3? spacer sequence. In yet another embodiment, the vector can also comprise the 3? spacer sequence, but not the 5? spacer sequence.

Abstract: An apparatus for making tongue and groove joints which includes a handle having a gripping end and an attachment end. A drive motor is disposed within the handle. An output shaft extends perpendicular to the handle at the attachment end. A cutting guide overlies the output shaft. The cutting guide has a first step which is planar so as to rest upon a flat working surface, a second step and a riser that extends between the first step and the second step. This riser is perpendicular to the first step. A cutting blade is rotatably mounted on the output shaft and projects from the riser parallel to the first step and in an intermediate position between the first step and the second step. The blade is adapted to cut a groove of a selected depth and width in a board.