Patents by Inventor Daniel G. Olson

Daniel G. Olson has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 10767196
    Abstract: The present invention provides for the manipulation of cofactor usage in a recombinant host cell to increase the formation of desirable products. In some embodiments, the invention provides for a recombinant microorganism comprising a mutation in one or more native enzymes such that their cofactor specificity is altered in such a way that overall cofactor usage in the cell is balanced for a specified pathway and there is an increase in a specific product formation within the cell. In some embodiments, endogenous enzymes are replaced by enzymes with an alternate cofactor specificity from a different species.
    Type: Grant
    Filed: November 30, 2012
    Date of Patent: September 8, 2020
    Assignees: Enchi Corporation, Dartmouth College, UT-Battelle, LLC
    Inventors: Jonathan Lo, Adam M. Guss, Johannes P. Van Dijken, Arthur J. Shaw, IV, Daniel G. Olson, Christopher D. Herring, D. Aaron Argyros, Nicky Caiazza
  • Patent number: 10619172
    Abstract: Disclosed are methods for engineering bacteria, for example, Thermoanaerobacterium saccharolyticum, that convert biomass to ethanol at high yield by deleting a single gene. Deletion of subunit A or subunit B of the hfs hydrogenase, but not deletion of subunit C or subunit D, results in an increase in ethanol yield.
    Type: Grant
    Filed: July 21, 2017
    Date of Patent: April 14, 2020
    Assignee: THE TRUSTEES OF DARTMOUTH COLLEGE
    Inventors: Aysenur Eminoglu, Daniel G. Olson, Lee R. Lynd
  • Publication number: 20180195091
    Abstract: It is disclosed here engineered cellulolytic microorganisms capable of producing ethanol from lignocellulosic feedstock with high yield. Multiple genes in Thermoanaerobacterium saccharolyticum that are involved in the pyruvate to ethanol pathway are disclosed which may be transferred into C. thermocellum or other natively cellulolytic microorganisms.
    Type: Application
    Filed: July 23, 2016
    Publication date: July 12, 2018
    Inventors: Daniel G. Olson, Lee R. Lynd, Liang TIAN, Jonathan LO, Jilai ZHOU, Shuen HON, Tianyong ZHENG
  • Publication number: 20180023100
    Abstract: Disclosed are methods for engineering bacteria, for example, Thermoanaerobacterium saccharolyticum, that convert biomass to ethanol at high yield by deleting a single gene. Deletion of subunit A or subunit B of the hfs hydrogenase, but not deletion of subunit C or subunit D, results in an increase in ethanol yield.
    Type: Application
    Filed: July 21, 2017
    Publication date: January 25, 2018
    Inventors: Aysenur Eminoglu, Daniel G. Olson, Lee R. Lynd
  • Patent number: 9803221
    Abstract: The present invention provides for the manipulation of carbon flux in a recombinant host cell to increase the formation of desirable products. The invention relates to cellulose-digesting organisms that have been genetically modified to allow the production of ethanol at a high yield by redirecting carbon flux at key steps of central metabolism.
    Type: Grant
    Filed: September 28, 2012
    Date of Patent: October 31, 2017
    Assignees: Enchi Corporation, Dartmouth College
    Inventors: Yu Deng, Daniel G. Olson, Johannes Pieter van Dijken, Arthur J. Shaw, IV, Aaron Argyros, Trisha Barrett, Nicky Caiazza, Christopher D. Herring, Stephen R. Rogers, Frank Agbogbo
  • Publication number: 20140356921
    Abstract: The present invention provides for the manipulation of carbon flux in a recombinant host cell to increase the formation of desirable products. The invention relates to cellulose-digesting organisms that have been genetically modified to allow the production of ethanol at a high yield by redirecting carbon flux at key steps of central metabolism.
    Type: Application
    Filed: September 28, 2012
    Publication date: December 4, 2014
    Applicant: Mascoma Corporation
    Inventors: Yu Deng, Daniel G. Olson, Johannes Pieter van Dijken, Arthur J. Shaw, IV, Aaron Argyros, Trisha Barrett, Nicky Caiazza, Christopher D. Herring, Stephen R. Rogers, Frank Agbogbo
  • Publication number: 20140322783
    Abstract: The present invention provides for the manipulation of cofactor usage in a recombinant host cell to increase the formation of desirable products. In some embodiments, the invention provides for a recombinant microorganism comprising a mutation in one or more native enzymes such that their cofactor specificity is altered in such a way that overall cofactor usage in the cell is balanced for a specified pathway and there is an increase in a specific product formation within the cell. In some embodiments, endogenous enzymes are replaced by enzymes with an alternate cofactor specificity from a different species.
    Type: Application
    Filed: November 30, 2012
    Publication date: October 30, 2014
    Inventors: Jonathan Lo, Adam M. Guss, Johannes P. Van Dijken, Arthur J. Shaw, IV, Daniel G. Olson, Christopher D. Herring
  • Publication number: 20110281362
    Abstract: The present invention relates to methods for transforming Gram-positive, anaerobic, thermophilic bacteria via electroporation and Gram-positive, anaerobic, thermophilic bacteria transformed by the disclosed methods. The methods employ voltage pulsing schemes that decrease arcing such that increased transformation efficiency and cell viability is observed. The present invention is further directed to a method for transforming Gram-positive, anaerobic, thermophilic bacteria via electroporation using recovery/selection temperatures to effect increased transformation efficiency in difficult to transform bacteria.
    Type: Application
    Filed: October 13, 2009
    Publication date: November 17, 2011
    Applicant: Mascoma Corporation
    Inventor: Daniel G. Olson