Abstract: A system and method for remediating a body of water and collecting suspended and dissolved solids therefrom are provided. The system includes a water-impervious lining positionable in a depression in or adjacent a body of water. The lining and depression define a treatment vessel, which includes a treatment portion, an outlet portion for containing treated water, and an outflow weir between the treatment portion and the outlet portion. Water to be treated is transported from the water body to the treatment portion. An entrapment element is delivered and mixed into the transported water, the entrapment element for capturing suspended and dissolved solids in the transported water and effecting a separation between the captured solids and water cleansed therefrom. The captured solids can be removed from the treatment basin, and the cleansed water can move through a channel in the outflow weir into the outlet portion.

Abstract: A door threshold shield apparatus includes a shield body shaped for covering a portion of a door threshold. The shield body can include a sheet metal or other suitable material. A hook is positioned on a first longitudinal edge of the shield body for retaining the shield body on a first edge of the threshold, and a cap flange is positioned on a second longitudinal edge of the shield body. The cap flange is shaped to be received between a removable cap flange on the threshold and a threshold base, thereby rigidly securing the shield apparatus to the threshold. Once construction is complete, the shield may be removed without removing the threshold base by detaching the threshold cap.

Abstract: A system and method for remediating a body of water and collecting suspended and dissolved solids therefrom are provided. The system includes a water-impervious lining positionable in a depression in or adjacent a body of water. The lining and depression define a treatment vessel, which includes a treatment portion, an outlet portion for containing treated water, and an outflow weir between the treatment portion and the outlet portion. Water to be treated is transported from the water body to the treatment portion. An entrapment element is delivered and mixed into the transported water, the entrapment element for capturing suspended and dissolved solids in the transported water and effecting a separation between the captured solids and water cleansed therefrom. The captured solids can be removed from the treatment basin, and the cleansed water can move through a channel in the outflow weir into the outlet portion.

Abstract: A system and method for remediating a body of water and collecting suspended and dissolved solids therefrom are provided. The system includes a water-impervious lining positionable in a depression in or adjacent a body of water. The lining and depression define a treatment vessel, which includes a treatment portion, an outlet portion for containing treated water, and an outflow weir between the treatment portion and the outlet portion. Water to be treated is transported from the water body to the treatment portion. An entrapment element is delivered and mixed into the transported water, the entrapment element for capturing suspended and dissolved solids in the transported water and effecting a separation between the captured solids and water cleansed therefrom. The captured solids can be removed from the treatment basin, and the cleansed water can move through a channel in the outflow weir into the outlet portion.

Abstract: A system and method for remediating a body of water and collecting suspended and dissolved solids therefrom are provided. The system includes a water-impervious lining positionable in a depression in or adjacent a body of water. The lining and depression define a treatment vessel, which includes a treatment portion, an outlet portion for containing treated water, and an outflow weir between the treatment portion and the outlet portion. Water to be treated is transported from the water body to the treatment portion. An entrapment element is delivered and mixed into the transported water, the entrapment element for capturing suspended and dissolved solids in the transported water and effecting a separation between the captured solids and water cleansed therefrom. The captured solids can be removed from the treatment basin, and the cleansed water can move through a channel in the outflow weir into the outlet portion.

Abstract: This invention relates to spotting solutions for the production of assay articles with uniform spot size and morphology for the detection of biopolymers, comprising N-lauroyl sarcosine in a buffered solution. Optionally the spotting solution may further comprise a fluorophore or a dye. This invention further provides means for non-destructive quality control of the production of said assay articles. This invention further provides means for non-destructive quality control of the production of said assay articles.

Abstract: The present invention provides a useful system for assays that comprises a solid support, a plurality of capture oligonucleotides immobilized onto the solid support, and complementary oligonucleotides attached to capture ligands. A detectable label can be directly attached to the capture oligonucleotides or the complementary oligonucleotides. The labeled oligonucleotides can be detected, and used to determine the quality of the assay. A labeled detector ligand corresponding to a target ligand can also be independently detected apart from the labeled oligonucleotide.

Abstract: The present invention provides a useful system for assays that comprises a solid support, a plurality of capture oligonucleotides immobilized onto the solid support, and complementary oligonucleotides attached to capture ligands. A detectable label can be directly attached to the capture oligonucleotides or the complementary oligonucleotides. The labeled oligonucleotides can be detected, and used to determine the quality of the assay. A labeled detector ligand corresponding to a target ligand can also be independently detected apart from the labeled oligonucleotide.

Abstract: The invention is an oligonucleotide-based assay system and kit useful for sorting, detecting, and identifying analytes. The system utilizes complementary oligonucleotide pairs in which one oligonucleotide of each pair is immobilized to a solid substrate and the other oligonucleotide has an analyte binding agent attached to it. The different oligonucleotide pairs hybridize at substantially the same rate, have substantially the same Tm, have nucleotide sequences designed to minimize cross-hybridization between different pairs, and hybridize together relatively rapidly at ambient temperatures without detectable cross-hybridization.

Abstract: The present invention is in the field of chemistry and biotechnology. The present invention relates to cell-based microarrays, improved methods for forming such arrays, and methods for using such arrays in diagnostics, therapeutics and research. The invention particularly concerns microarrays in which ligands of a target cells are immobilized to the array support via ligand-binding molecules bound to an oligonucleotide that is hybridized to a support-immobilized oligonucleotide.

Abstract: This invention relates to methods for increasing the dynamic range and accuracy of assays in which the presence, absence, activity or concentration of a target analyte is assayed by the emission or quenching of a light signal, or by a change (i.e., an evolution or loss) of a light signal in two or more time intervals. In preferred embodiments multiple digitized images are captured at varying times, and the images analyzed to identify captured images within the dynamic range of the assay. The invention further relates to apparati capable of implementing such methods.

Abstract: This invention relates to methods for increasing the dynamic range and accuracy of assays in which the presence, absence, activity or concentration of a target analyte is assayed by the emission or quenching of a light signal, or by a change (i.e., an evolution or loss) of a light signal in two or more time intervals. In preferred embodiments multiple digitized images are captured at varying times, and the images analyzed to identify captured images within the dynamic range of the assay. The invention further relates to apparati capable of implementing such methods.

Abstract: The present invention provides a system using internal reference spots to reduce microarray assay variation. A microarray device for quantifying a plurality of target ligands in a sample includes a solid support having a plurality of test areas; a plurality of different target capture ligands immobilized onto the test areas; and at least one reference capture ligand immobilized onto the test areas. The reference capture ligand captures a reference ligand not normally present in the sample.

Abstract: The present invention provides a useful system for assays that comprises a solid support, a plurality of capture oligonucleotides immobilized onto the solid support, and complementary oligonucleotides attached to capture ligands. A detectable label can be directly attached to the capture oligonucleotides or the complementary oligonucleotides. The labeled oligonucleotides can be detected, and used to determine the quality of the assay. A labeled detector ligand corresponding to a target ligand can also be independently detected apart from the labeled oligonucleotide.

Abstract: The invention is an oligonucleotide-based assay system and kit useful for sorting, detecting, and identifying analytes. The system utilizes complementary oligonucleotide pairs in which one oligonucleotide of each pair is immobilized to a solid substrate and the other oligonucleotide has an analyte binding agent attached to it. The different oligonucleotide pairs hybridize at substantially the same rate, have substantially the same Tm, have nucleotide sequences designed to minimize cross-hybridization between different pairs, and hybridize together relatively rapidly at ambient temperatures without detectable cross-hybridization.

Abstract: Oligonucleotide probes, kits, and methods useful for detecting a polynucleotide target in a sample are provided. The method, a mixture is formed by combining a polynucleotide target sample, a first probe that is complementary to the polynucleotide target and having a first fluorescent donor or fluorescent acceptor; and a second probe that is partially complementary to the first probe and having a second fluorescent donor or fluorescent acceptor. The second probe competes with the polynucleotide target for binding to the first probe and the first probe preferentially binds to the polynucleotide target rather than to the second probe. The-first fluorescent donor or acceptor and second fluorescent donor or acceptor form a donor/acceptor pair capable of fluorescence resonance energy transfer (FRET) with each other in response to activation of the fluorescent donor by light of a predetermined wavelength or band of wavelengths.

Abstract: Oligonucleotide probes, kits, and methods useful for detecting a polynucleotide target in a sample are provided. The method, a mixture is formed by combining a polynucleotide target sample, a first probe that is complementary to the polynucleotide target and having a first fluorescent donor or fluorescent acceptor; and a second probe that is partially complementary to the first probe and having a second fluorescent donor or fluorescent acceptor. The second probe competes with the polynucleotide target for binding to the first probe, and the first probe preferentially binds to the polynucleotide target rather than to the second probe. The first fluorescent donor or acceptor and second fluorescent donor or acceptor form a donor/acceptor pair capable of fluorescence resonance energy transfer (FRET) with each other in response to activation of the fluorescent donor by light of a predetermined wavelength or band of wavelengths.

Abstract: This invention relates to methods for increasing the dynamic range and accuracy of assays in which the presence, absence, activity or concentration of a target analyte is assayed by the emission or quenching of a light signal, or by a change (i.e., an evolution or loss) of a light signal in two or more time intervals. In preferred embodiments multiple digitized images are captured at varying times, and the images analyzed to identify captured images within the dynamic range of the assay. The invention further relates to apparati capable of implementing such methods.

Abstract: Reagents and methods for preparing samples containing both biomolecule analytes and macrobiomolecules for capillary electrophoresis separation are provided. The reagents comprise a branched polymer. When mixed with a sample containing both biomolecule analytes and macrobiomolecules, particularly DNA templates, the branched polymer of the reagent can suppress the entrance of the macrobiomolecules into a capillary electrophoresis tube during electrokinetic injection of the sample into the capillary electrophoresis tube.