Patents by Inventor Daniel Kolczynski

Daniel Kolczynski has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Carbohydrate binding modules with reduced binding to lignin
    Patent number: 9885028
    Abstract: Provided is a modified Family 1 carbohydrate binding module (CBM) comprising amino acid substitutions at one or more of positions 10, 11, 12, 14, 17, 21, 24, 29, 31, 33, and 37, said position determined from alignment of a Family 1 CBM amino acid sequence with SEQ ID NO: 30, and exhibiting from about 50% to about 99.9% amino acid sequence identity to SEQ ID NO: 30. Also provided are modified glycosidase enzymes comprising the modified Family 1 CBM, genetic constructs and genetically modified microbes for expressing the modified Family 1 CBM or modified glycosidase enzyme. The modified Family 1 CBM confers reduced lignin binding and/or increased hydrolyzing activity in the presence of lignin to the modified glycosidase enzyme, which may be used in a process for hydrolyzing cellulose or hemicellulose in the presence of lignin.
    Type: Grant
    Filed: October 21, 2015
    Date of Patent: February 6, 2018
    Assignee: Iogen Energy Corporation
    Inventors: John J. Tomashek, Brian R. Scott, Daniel Kolczynski
  • CARBOHYDRATE BINDING MODULES WITH REDUCED BINDING TO LIGNIN
    Publication number: 20160115464
    Abstract: Provided is a modified Family 1 carbohydrate binding module (CBM) comprising amino acid substitutions at one or more of positions 10, 11, 12, 14, 17, 21, 24, 29, 31, 33, and 37, said position determined from alignment of a Family 1 CBM amino acid sequence with SEQ ID NO: 30, and exhibiting from about 50% to about 99.9% amino acid sequence identity to SEQ ID NO: 30. Also provided are modified glycosidase enzymes comprising the modified Family 1 CBM, genetic constructs and genetically modified microbes for expressing the modified Family 1 CBM or modified glycosidase enzyme. The modified Family 1 CBM confers reduced lignin binding and/or increased hydrolysing activity in the presence of lignin to the modified glycosidase enzyme, which may be used in a process for hydrolysing cellulose or hemicellulose in the presence of lignin.
    Type: Application
    Filed: October 21, 2015
    Publication date: April 28, 2016
    Inventors: John J. Tomashek, Brian R. Scott, Daniel Kolczynski
  • Carbohydate binding modules with reduced binding to lignin
    Patent number: 9206406
    Abstract: Provided is a modified Family 1 carbohydrate binding module (CBM) comprising amino acid substitutions at one or more of positions 10, 11, 12, 14, 17, 21, 24, 29, 31, 33, and 37, said position determined from alignment of a Family 1 CBM amino acid sequence with SEQ ID NO: 30, and exhibiting from about 50% to about 99.9% amino acid sequence identity to SEQ ID NO: 30. Also provided are modified glycosidase enzymes comprising the modified Family 1 CBM, genetic constructs and genetically modified microbes for expressing the modified Family 1 CBM or modified glycosidase enzyme. The modified Family 1 CBM confers reduced lignin binding and/or increased hydrolyzing activity in the presence of lignin to the modified glycosidase enzyme, which may be used in a process for hydrolyzing cellulose or hemicellulose in the presence of lignin.
    Type: Grant
    Filed: February 11, 2011
    Date of Patent: December 8, 2015
    Assignee: IOGEN ENERGY CORPORATION
    Inventors: John J. Tomashek, Brian R. Scott, Daniel Kolczynski
  • CARBOHYDATE BINDING MODULES WITH REDUCED BINDING TO LIGNIN
    Publication number: 20120295308
    Abstract: Provided is a modified Family 1 carbohydrate binding module (CBM) comprising amino acid substitutions at one or more of positions 10, 11, 12, 14, 17, 21, 24, 29, 31, 33, and 37, said position determined from alignment of a Family 1 CBM amino acid sequence with SEQ ID NO: 30, and exhibiting from about 50% to about 99.9% amino acid sequence identity to SEQ ID NO: 30. Also provided are modified glycosidase enzymes comprising the modified Family 1 CBM, genetic constructs and genetically modified microbes for expressing the modified Family 1 CBM or modified glycosidase enzyme. The modified Family 1 CBM confers reduced lignin binding and/or increased hydrolysing activity in the presence of lignin to the modified glycosidase enzyme, which may be used in a process for hydrolysing cellulose or hemicellulose in the presence of lignin.
    Type: Application
    Filed: February 11, 2011
    Publication date: November 22, 2012
    Applicant: IOGEN ENEGY CORPORATION
    Inventors: John J. Tomashek, Brian R. Scott, Daniel Kolczynski