Patents by Inventor Daniel Korenblum
Daniel Korenblum has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20230295247Abstract: Described herein are variants of alpha-hemolysin having at least one mutation selected from T12R, T12K, N17R, N17K or combinations of T12 and N17 mutations. The variants in some embodiments may further comprise H144A. The ?-hemolysin variants have a decreased time to thread.Type: ApplicationFiled: September 21, 2022Publication date: September 21, 2023Inventors: Michael DORWART, Daniel KORENBLUM
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Patent number: 11499190Abstract: This disclosure provides chips, systems and methods for sequencing a nucleic acid sample. Tagged nucleotides are provided into a reaction chamber comprising a nanopore in a membrane. An individual tagged nucleotide of the tagged nucleotides can contain a tag coupled to a nucleotide, which tag is detectable with the aid of the nanopore. Next, an individual tagged nucleotide of the tagged nucleotides can be incorporated into a growing strand complementary to a single stranded nucleic acid molecule derived from the nucleic acid sample. With the aid of the nanopore, a tag associated with the individual tagged nucleotide can be detected upon incorporation of the individual tagged nucleotide. The tag can be detected with the aid of the nanopore when the tag is released from the nucleotide.Type: GrantFiled: May 29, 2020Date of Patent: November 15, 2022Assignee: Roche Sequencing Solutions, Inc.Inventors: Randall W. Davis, Roger J. A. Chen, Arkadiusz Bibillo, Daniel Korenblum
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Patent number: 11479584Abstract: Described herein are variants of alpha-hemolysin having at least one mutation selected from T12R, T12K, N17R, N17K or combinations of T12 and N17 mutations. The variants in some embodiments may further comprise H144A. The ?-hemolysin variants have a decreased time to thread.Type: GrantFiled: May 6, 2020Date of Patent: October 25, 2022Assignee: Roche Sequencing Solutions, Inc.Inventors: Michael Dorwart, Daniel Korenblum
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Publication number: 20200407786Abstract: This disclosure provides chips, systems and methods for sequencing a nucleic acid sample. Tagged nucleotides are provided into a reaction chamber comprising a nanopore in a membrane. An individual tagged nucleotide of the tagged nucleotides can contain a tag coupled to a nucleotide, which tag is detectable with the aid of the nanopore. Next, an individual tagged nucleotide of the tagged nucleotides can be incorporated into a growing strand complementary to a single stranded nucleic acid molecule derived from the nucleic acid sample. With the aid of the nanopore, a tag associated with the individual tagged nucleotide can be detected upon incorporation of the individual tagged nucleotide. The tag can be detected with the aid of the nanopore when the tag is released from the nucleotide.Type: ApplicationFiled: May 29, 2020Publication date: December 31, 2020Inventors: Randall W. Davis, Roger J.A. Chen, Arkadiusz Bibillo, Daniel Korenblum
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Patent number: 10822650Abstract: This disclosure provides chips, systems and methods for sequencing a nucleic acid sample. Tagged nucleotides are provided into a reaction chamber comprising a nanopore in a membrane. An individual tagged nucleotide of the tagged nucleotides can contain a tag coupled to a nucleotide, which tag is detectable with the aid of the nanopore. Next, an individual tagged nucleotide of the tagged nucleotides can be incorporated into a growing strand complementary to a single stranded nucleic acid molecule derived from the nucleic acid sample. With the aid of the nanopore, a tag associated with the individual tagged nucleotide can be detected upon incorporation of the individual tagged nucleotide. The tag can be detected with the aid of the nanopore when the tag is released from the nucleotide.Type: GrantFiled: March 27, 2017Date of Patent: November 3, 2020Assignee: Roche Sequencing Solutions, Inc.Inventors: Randall Davis, Roger Chen, Arkadiusz Bibillo, Daniel Korenblum
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Publication number: 20200339634Abstract: Described herein are variants of alpha-hemolysin having at least one mutation selected from T12R, T12K, N17R, N17K or combinations of T12 and N17 mutations. The variants in some embodiments may further comprise H144A. The ?-hemolysin variants have a decreased time to thread.Type: ApplicationFiled: May 6, 2020Publication date: October 29, 2020Applicant: Roche Sequencing Solutions, Inc.Inventors: Michael DORWART, Daniel KORENBLUM
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Patent number: 10683331Abstract: Described herein are variants of alpha-hemolysin having at least one mutation selected from T12R, T12K, N17R, N17K or combinations of T12 and N17 mutations. The variants in some embodiments may further comprise H144A. The ?-hemolysin variants have a decreased time to thread.Type: GrantFiled: April 4, 2019Date of Patent: June 16, 2020Assignee: Roche Sequencing Solutions, Inc.Inventors: Michael Dorwart, Daniel Korenblum
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Publication number: 20190382833Abstract: This disclosure provides chips, systems and methods for sequencing a nucleic acid sample. Tagged nucleotides are provided into a reaction chamber comprising a nanopore in a membrane. An individual tagged nucleotide of the tagged nucleotides can contain a tag coupled to a nucleotide, which tag is detectable with the aid of the nanopore. Next, an individual tagged nucleotide of the tagged nucleotides can be incorporated into a growing strand complementary to a single stranded nucleic acid molecule derived from the nucleic acid sample. With the aid of the nanopore, a tag associated with the individual tagged nucleotide can be detected upon incorporation of the individual tagged nucleotide. The tag can be detected with the aid of the nanopore when the tag is released from the nucleotide.Type: ApplicationFiled: December 20, 2017Publication date: December 19, 2019Inventors: Randall W. Davis, Roger J.A. Chen, Arkadiusz Bibillo, Daniel Korenblum
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Publication number: 20190225656Abstract: Described herein are variants of alpha-hemolysin having at least one mutation selected from T12R, T12K, N17R, N17K or combinations of T12 and N17 mutations. The variants in some embodiments may further comprise H144A. The ?-hemolysin variants have a decreased time to thread.Type: ApplicationFiled: April 4, 2019Publication date: July 25, 2019Applicant: Genia Technologies, Inc.Inventors: Michael Dorwart, Daniel Korenblum
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Patent number: 10301361Abstract: Described herein are variants of alpha-hemolysin having at least one mutation selected from T12R, T12K, N17R, N17K or combinations of T12 and N17 mutations. The variants in some embodiments may further comprise H144A. The ?-hemolysin variants have a decreased time to thread.Type: GrantFiled: October 28, 2015Date of Patent: May 28, 2019Assignee: GENIA TECHNOLOGIES, INC.Inventors: Michael Dorwart, Daniel Korenblum
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Publication number: 20180030524Abstract: This disclosure provides chips, systems and methods for sequencing a nucleic acid sample. Tagged nucleotides are provided into a reaction chamber comprising a nanopore in a membrane. An individual tagged nucleotide of the tagged nucleotides can contain a tag coupled to a nucleotide, which tag is detectable with the aid of the nanopore. Next, an individual tagged nucleotide of the tagged nucleotides can be incorporated into a growing strand complementary to a single stranded nucleic acid molecule derived from the nucleic acid sample. With the aid of the nanopore, a tag associated with the individual tagged nucleotide can be detected upon incorporation of the individual tagged nucleotide. The tag can be detected with the aid of the nanopore when the tag is released from the nucleotide.Type: ApplicationFiled: March 27, 2017Publication date: February 1, 2018Inventors: Randall Davis, Roger Chen, Arkadiusz Bibillo, Daniel Korenblum
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Publication number: 20170088588Abstract: Described herein are variants of alpha-hemolysin having at least one mutation selected from T12R, T12K, N17R, N17K or combinations of T12 and N17 mutations. The variants in some embodiments may further comprise H144A. The ?-hemolysin variants have a decreased time to thread.Type: ApplicationFiled: October 28, 2015Publication date: March 30, 2017Inventors: Michael DORWART, Daniel KORENBLUM
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Patent number: 9605309Abstract: This disclosure provides chips, systems and methods for sequencing a nucleic acid sample. Tagged nucleotides are provided into a reaction chamber comprising a nanopore in a membrane. An individual tagged nucleotide of the tagged nucleotides can contain a tag coupled to a nucleotide, which tag is detectable with the aid of the nanopore. Next, an individual tagged nucleotide of the tagged nucleotides can be incorporated into a growing strand complementary to a single stranded nucleic acid molecule derived from the nucleic acid sample. With the aid of the nanopore, a tag associated with the individual tagged nucleotide can be detected upon incorporation of the individual tagged nucleotide. The tag can be detected with the aid of the nanopore when the tag is released from the nucleotide.Type: GrantFiled: November 6, 2013Date of Patent: March 28, 2017Assignee: Genia Technologies, Inc.Inventors: Randall Davis, Roger Chen, Arkadiusz Bibillo, Daniel Korenblum
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Publication number: 20140134616Abstract: This disclosure provides chips, systems and methods for sequencing a nucleic acid sample. Tagged nucleotides are provided into a reaction chamber comprising a nanopore in a membrane. An individual tagged nucleotide of the tagged nucleotides can contain a tag coupled to a nucleotide, which tag is detectable with the aid of the nanopore. Next, an individual tagged nucleotide of the tagged nucleotides can be incorporated into a growing strand complementary to a single stranded nucleic acid molecule derived from the nucleic acid sample. With the aid of the nanopore, a tag associated with the individual tagged nucleotide can be detected upon incorporation of the individual tagged nucleotide. The tag can be detected with the aid of the nanopore when the tag is released from the nucleotide.Type: ApplicationFiled: November 6, 2013Publication date: May 15, 2014Applicant: Genia Technologies, Inc.Inventors: Randall Davis, Roger Chen, Arkadiusz Bibillo, Daniel Korenblum, Michael Dorwart