Abstract: The invention is a method using immunofluorescence, high-resolution imaging, and 3D image analysis to quantify spatial distribution of methylcytosine (MeC) in global DNA in individual cell nuclei, for the characterization of cells and tissues based on their nuclear MeC distribution patterns. The invented method is intended for basic research, clinical diagnostics and prognostics, pharmacology and toxicology, and molecular and cell-based therapy. We describe an image-cytometrical tool performing rapid cell-by-cell assay to analyze the impact of environmental conditions on global DNA methylation, including alterations of methylcytosine load and the spatial reorganization of methylated DNA in cell nuclei. The assay can be combined with molecular approaches for profiling of cells and their genomes. In contrast to molecular methods, it provides information regarding the location of MeC in cell nuclei.

Abstract: The subject matter disclosed herein relates to the field of spectral imaging in the diagnosis and treatment of Hirschsprung's disease. Devices and methods are provided that enhance and accurately diagnose Hirschsprung's disease intraoperatively using spectral imaging technology.

Abstract: An apparatus for image elastic scattering spectroscopy is disclosed that is comprised of a light source for generating polarized light. Means are provided to convey the polarized light to a target. A collector receives light reflected from the target. A detector is responsive to the collector for generating images at both parallel and perpendicular polarizations for each of a plurality of wavelengths. A range finder detects a distance to the target. Control electronics control the image generation and the range finder. The apparatus may be configured to image areas on the surface of the body or configured so as to be inserted into various body cavities. Typically, the apparatus will be used in conjunction with an analyzer for analyzing the images for evidence of abnormal cells. Methods of gathering data and of screening for abnormal cells are also disclosed.

Abstract: The present disclosure involves a method of preparing a cervical sample for medical examination in which a papanicolaou stained sample is additionally stained with a label targeted to one or more high risk cancer markers to allow for optical detection of the human papilloma virus. That optical detection can be combined with morphometric detection of abnormal cells, which may be improved by multispectral imaging using an acousto-optic tunable filter. Several variations of screening methods and an apparatus for performing the methods are disclosed.

Abstract: The present invention is in part directed molecular modeling. In one aspect, a method for determining a structure of a protein is provided, comprising determining the minimum excluded volume of said protein. In another aspect, a method for identifying molecules is provided. In yet another aspect, a computer product is provided for determining the structure of a protein.

Abstract: A light microscope comprises a light source and a first acousto-optic tunable filter responsive to the light source for producing two light streams of different polarization. A control circuit is provided for tuning the first filter to a plurality of frequencies. A mechanism, such as steering optics, is provided for combining the two light streams into a combined light stream. Input optics focus the combined light stream. A condenser, such as a darkfield condenser, receives the focused, combined, light stream and projects it onto a sample. A second acousto-optic tunable filter is responsive to light from the sample. A second control circuit is provided for tuning the second acousto-optic filter to a plurality of frequencies. The light from the second acousto-optic tunable filter may be captured and stored for future processing.

Abstract: An imaging system comprises an acousto-optic tunable filter for receiving light at an input end thereof and outputting light at an output end thereof. A control circuit is provided for tuning the filter. A prism is responsive to the light output by the tunable filter. The prism is oriented at an angle with respect to the filter to compensate for dispersion of the output light caused by the tunable filter.