Abstract: Systems, methods and devices are provided for electro-osmotic propulsion in a microfluidic environment. These systems, methods and devices can include a body having a channel comprising a pair of open ends, and a plurality of electrodes coupled to the body, wherein the electrodes are configured to generate a voltage and cause an electro-osmotic flow of a fluid through the channel. In many of the embodiments, an on-board power supply coupled to the body is provided for generating voltage across the electrodes. In some embodiments, the channel comprises a cylindrical shape having a circular cross-sectional area.