Patents by Inventor Daniel L. Woodard
Daniel L. Woodard has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 5625054Abstract: The present invention relates to fluorinated surfaces which exhibit sufficient hydrophilicity and sufficient electropositivity to bind DNA from a suspension containing DNA and permit elution of the DNA from the surface. Generally, the hydrophilic and electropositive characteristics are expressed at the fluorinated surface. Preferred fluorinated surfaces of the present invention include fluorinated Al(OH).sub.3, fluorinated SiO.sub.2 and fluorinated Celite. The fluorinated surfaces of the present invention are particularly useful in processes for purification of DNA from other cellular components. In these processes, a suspension of cellular components is placed in contact with the fluorinated surface, the fluorinated surface is washed to remove all cellular components other than DNA which are bound to the surface, and the bound DNA is eluted from the surface. Lower concentrations of chaotrope in the binding buffer are needed to bind DNA to the fluorinated surfaces.Type: GrantFiled: May 25, 1995Date of Patent: April 29, 1997Assignee: Becton Dickinson and CompanyInventors: Daniel L. Woodard, Adriann J. Howard, James A. Down
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Patent number: 5620869Abstract: It has been found that certain glycoproteins, particularly mucins, are inhibitors of nucleic acid amplification reactions and that inhibition of the amplification reaction is associated with partial degradation of the carbohydrate chain. Partial degradation of the carbohydrate of a non-inhibitory glycoprotein renders it inhibitory, and partial degradation of the carbohydrate of a slightly inhibitory glycoprotein makes it more inhibitory. Sample processing prior to amplification may contribute to partial degradation of the carbohydrate chains of the glycoproteins which are present and increase their inhhibitory effect. In contrast, complete removal of the carbohydrate significantly reduces or completely eliminates the inhibitory effect. Methods for reducing or eliminating glycoprotein-associated inhibition of nucleic acid amplification reactions are also disclosed.Type: GrantFiled: September 28, 1995Date of Patent: April 15, 1997Assignee: Becton, Dickinson and CompanyInventors: Daniel L. Woodard, Adriann H. Walters, Michael C. Little
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Patent number: 5616701Abstract: The present invention relates to modified glass fiber membranes which exhibit sufficient hydrophilicity and sufficient electropositivity to bind DNA from a suspension containing DNA and permit elution of the DNA from the membrane. Generally, the hydrophilic and electropositive characteristics are expressed at the surface of the modified glass fiber membrane. Preferred modified glass fiber membranes of the present invention include glass fiber membranes that have been modified by treatment with trifluoroacetic acid (TFA), BCl.sub.3, SiCl.sub.4, NaOH, F.sup.-, AlCl.sub.3 alone or in combination, with or without water. The modified glass fiber membranes of the present invention are particularly useful in processes for purification of DNA from other cellular components.Type: GrantFiled: May 24, 1995Date of Patent: April 1, 1997Assignee: Becton Dickinson and CompanyInventors: Daniel L. Woodard, Adriann J. Howard, James A. Down
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Patent number: 5610291Abstract: The present invention relates to modified glass fiber membranes which exhibit sufficient hydrophilicity and sufficient electropositivity to bind DNA from a suspension containing DNA and permit elution of the DNA from the membrane. Generally, the hydrophilic and electropositive characteristics are expressed at the surface of the modified glass fiber membrane. Preferred modified glass fiber membranes of the present invention include glass fiber membranes that have been modified by treatment with trifluoroacetic acid (TFA), BCl.sub.3, SiCl.sub.4, NaOH, F.sup.-, AlCl.sub.3 alone or in combination, with or without water. The modified glass fiber membranes of the present invention are particularly useful in processes for purification of DNA from other cellular components.Type: GrantFiled: May 25, 1995Date of Patent: March 11, 1997Assignee: Becton Dickinson and CompanyInventors: Daniel L. Woodard, Adriann J. Howard, James A. Down
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Patent number: 5610290Abstract: The present invention relates to modified glass fiber membranes which exhibit sufficient hydrophilicity and sufficient electropositivity to bind DNA from a suspension containing DNA and permit elution of the DNA from the membrane. Generally, the hydrophilic and electropositive characteristics are expressed at the surface of the modified glass fiber membrane. Preferred modified glass fiber membranes of the present invention include glass fiber membranes that have been modified by treatment with trifluoroacetic acid (TFA), BCl.sub.3, SiCl.sub.4, NaOH, F.sup.-, AlCl.sub.3 alone or in combination, with or without water. The modified glass fiber membranes of the present invention are particularly useful in processes for purification of DNA from other cellular components.Type: GrantFiled: May 25, 1995Date of Patent: March 11, 1997Assignee: Becton Dickinson and CompanyInventors: Daniel L. Woodard, Adriann J. Howard, James A. Down
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Patent number: 5606046Abstract: The present invention relates to modified glass fiber membranes which exhibit sufficient hydrophilicity and sufficient electropositivity to bind DNA from a suspension containing DNA and permit elution of the DNA from the membrane. Generally, the hydrophilic and electropositive characteristics are expressed at the surface of the modified glass fiber membrane. Preferred modified glass fiber membranes of the present invention include glass fiber membranes that have been modified by treatment with trifluoroacetic acid (TFA), BCl.sub.3, SiCl.sub.4, NaOH, F.sup.-, AlCl.sub.3 alone or in combination, with or without water. The modified glass fiber membranes of the present invention are particularly useful in processes for purification of DNA from other cellular components.Type: GrantFiled: May 24, 1995Date of Patent: February 25, 1997Assignee: Becton Dickinson and CompanyInventors: Daniel L. Woodard, Adriann J. Howard, James A. Down
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Patent number: 5583217Abstract: Chromogenic and fluorogenic substrates for .beta.-lactamase, methods for synthesis thereof and methods for detecting .beta.-lactamase in a sample are provided. The substrates are substantially colorless or substantially nonfluorescent .beta.-lactam compounds which include an electronegative leaving group. The leaving group comprises a carbamate, carbonate, thiocarbamate or thiocarbonate linkage and a fluorescent moiety or a moiety capable of producing a visually detectable colored product. Upon cleavage of the lactam ring by .beta.-lactamase, the leaving group is liberated and fluorescence or a colored product is produced.Type: GrantFiled: April 6, 1995Date of Patent: December 10, 1996Assignee: Becton, Dickinson and CompanyInventors: J. Michael Quante, Randal A. Hoke, Patrick D. Mize, Daniel L. Woodard, O. Elmo Millner, deceased
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Patent number: 5534054Abstract: Silicon tetrahydrazide compounds produced by reacting SiCl.sub.4 with hydrazine to completion. The compounds are useful for binding DNA, as they do not require the use of chaotrope or alcohol solutions for binding. Binding and elution of DNA can be accomplished in low salt buffers or water. Kits containing the compounds for purification of DNA are also disclosed.Type: GrantFiled: March 9, 1994Date of Patent: July 9, 1996Assignee: Becton, Dickinson and CompanyInventors: Daniel L. Woodard, Adriann J. Howard
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Patent number: 5525319Abstract: Boron silicates, phosphosilicates and aluminum silicates useful as binding surfaces for DNA purification. These compounds allow DNA to be bound and eluted under native conditions (i.e., in the absence of chaotropes or alcohols) using only water, low salt buffers or physiological buffers. Methods for preparation of the compounds and methods for purifying DNA using the compounds are also provided.Type: GrantFiled: March 11, 1994Date of Patent: June 11, 1996Assignee: Becton, Dickinson and CompanyInventors: Daniel L. Woodard, Adriann J. Howard, James A. Down
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Patent number: 5523392Abstract: A process for purifying DNA comprising binding the DNA to a silicate compound in the presence of water, low salt buffer, or physiological buffer and eluting in the bound DNA by heating in a low salt buffer, a physiological buffer, or water. The silicate compound is prepared by reacting SiCl.sub.4 with a molar percentage of BCl.sub.3 of about 33.5%, 55.6%, or 83.3%, with a molar percentage of PCl.sub.3 of about 50.0%-83.3%, or with a molar percentage of AlCl.sub.3 of about 9.1%; cooling the reaction mixture; adding water until the evolution of gas is complete; and recovering the compound.Type: GrantFiled: March 11, 1994Date of Patent: June 4, 1996Assignee: Becton, Dickinson and CompanyInventors: Daniel L. Woodard, Adriann J. Howard, James A. Down
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Patent number: 5520899Abstract: A process for preparing borosilicate, aluminosilicate, or phosphosilicate comprising preparing a mixture of SiCl.sub.4 with a specific molar ratio of BCl.sub.3, AlCl.sub.3, or PCl.sub.3 ; cooling said mixture to zero degrees; and adding water to the reaction mixture until the evolution of gas is complete. These silicates are useful for purifying DNA wherein the DNA is bound in water or low salt buffers (i.e., in the absence of a chaotropic agent or alcohol).Type: GrantFiled: April 20, 1995Date of Patent: May 28, 1996Assignee: Becton, Dickinson and CompanyInventors: Daniel L. Woodard, Adriann J. Howard, James A. Down
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Patent number: 5516902Abstract: Chromogenic and fluorogenic substrates for .beta.-lactamase, methods for synthesis thereof and methods for detecting .beta.-lactamase in a sample are provided. The substrates are substantially colorless or substantially nonfluorescent .beta.-lactam compounds which include an electronegative leaving group. The leaving group comprises a carbamate, carbonate, thiocarbamate or thiocarbonate linkage and a fluorescent moiety or a moiety capable of producing a visually detectable colored product. Upon cleavage of the lactam ring by .beta.-lactamase, the leaving group is liberated and fluorescence or a colored product is produced.Type: GrantFiled: April 14, 1994Date of Patent: May 14, 1996Assignee: Becton, Dickinson and CompanyInventors: J. Michael Quante, Randal A. Hoke, Patrick D. Mize, Daniel L. Woodard, O. Elmo Millner, deceased
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Patent number: 5514561Abstract: Chromogenic and fluorogenic substrates for .beta.-lactamase, methods for synthesis thereof and methods for detecting .beta.-lactamase in a sample are provided. The substrates are substantially colorless or substantially nonfluorescent .beta.-lactam compounds which include an electronegative leaving group. The leaving group comprises a carbamate, carbonate, thiocarbamate or thiocarbonate linkage and a fluorescent moiety or a moiety capable of producing a visually detectable colored product. Upon cleavage of the lactam ring by .beta.-lactamase, the leaving group is liberated and fluorescence or a colored product is produced.Type: GrantFiled: April 14, 1994Date of Patent: May 7, 1996Assignee: Becton, Dickinson and CompanyInventors: J. Michael Quante, Randal A. Hoke, Patrick D. Mize, Daniel L. Woodard, O. Elmo Millner, deceased
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Patent number: 5503816Abstract: The present invention relates to a silicon-containing material which exhibits sufficient hydrophilicity and sufficient electropositivity to bind DNA from a suspension containing DNA and permit elution of the DNA from the material. Generally, the hydrophilic and electropositive characteristics are expressed at the surface of the silicon-containing material. Preferred silicon-containing materials of the present invention include boron silicate, aluminum silicate, phosphosilicate, silica carbonyl, silica sulfonyl and silica phosphonyl. The silicon-containing materials of the present invention are particularly useful in processes for purification of DNA from other cellular components. In these processes, a suspension of cellular components is placed in contact with the silicon-containing material, the silicon-containing material is washed to remove all cellular components other than DNA which are bound to the material, and the bound DNA is eluted from the material.Type: GrantFiled: September 27, 1993Date of Patent: April 2, 1996Assignee: Becton Dickinson and CompanyInventors: Daniel L. Woodard, Adriann J. Howard, James A. Down
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Patent number: 5438127Abstract: The present invention relates to modified glass fiber membranes which exhibit sufficient hydrophilicity and sufficient electropositivity to bind DNA from a suspension containing DNA and permit elution of the DNA from the membrane. Generally, the hydrophilic and electropositive characteristics are expressed at the surface of the modified glass fiber membrane. Preferred modified glass fiber membranes of the present invention include glass fiber membranes that have been modified by treatment with trifluoroacetic acid (TFA), BCl.sub.3, SiCl.sub.4, NaOH, F.sup.-, AlCl.sub.3 alone or in combination, with or without water. The modified glass fiber membranes of the present invention are particularly useful in processes for purification of DNA from other cellular components.Type: GrantFiled: September 27, 1993Date of Patent: August 1, 1995Assignee: Becton Dickinson and CompanyInventors: Daniel L. Woodard, Adriann J. Howard, James A. Down
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Patent number: 5438129Abstract: The present invention relates to fluorinated surfaces which exhibit sufficient hydrophilicity and sufficient electropositivity to bind DNA from a suspension containing DNA and permit elution of the DNA from the surface. Generally, the hydrophilic and electropositive characteristics are expressed at the fluorinated surface. Preferred fluorinated surfaces of the present invention include fluorinated Al(OH).sub.3, fluorinated SiO.sub.2 and fluorinated Celite. The fluorinated surfaces of the present invention are particularly useful in processes for purification of DNA from other cellular components. In these processes, a suspension of cellular components is placed in contact with the fluorinated surface, the fluorinated surface is washed to remove all cellular components other than DNA which are bound to the surface, and the bound DNA is eluted from the surface. Lower concentrations of chaotrope in the binding buffer are needed to bind DNA to the fluorinated surfaces.Type: GrantFiled: September 27, 1993Date of Patent: August 1, 1995Assignee: Becton Dickinson and CompanyInventors: Daniel L. Woodard, Adriann J. Howard, James A. Down
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Patent number: 5405951Abstract: The invention provides a method for purifying DNA from any source in any form. The method comprises the use of water soluble organic solvents when purifying DNA. By using water soluble organic solvents such as ethanol, propanol, and isopropanol, DNA is purified with greater recovery amounts. In addition, the use of water soluble organic solvents eliminates the use of caustic and poisonous compositions such as chaotropes.Type: GrantFiled: June 23, 1993Date of Patent: April 11, 1995Assignee: Becton Dickinson and CompanyInventor: Daniel L. Woodard
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Patent number: 5342931Abstract: A process for purifying DNA comprising 1) binding the DNA to a hydrated silica in the presence of water or physiological buffers in which the hydrated silica is prepared by refluxing silicon dioxide in sodium hydroxide or potassium hydroxide at a molar ratio of about 2:1 to 10:1 for at least about 48 hours, 2) separating and washing hydrated silica and the DNA bound thereto, and eluting the DNA from the hydrated silica in a heated physiological buffer or in heated water.Type: GrantFiled: April 23, 1993Date of Patent: August 30, 1994Assignee: Becton, Dickinson and CompanyInventors: Daniel L. Woodard, Adriann J. Howard, James A. Down
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Patent number: 5338843Abstract: Chromogenic and fluorogenic substrates for .beta.-lactamase, methods for synthesis thereof and methods for detecting .beta.-lactamase in a sample are provided. The substrates are substantially colorless or substantially nonfluorescent .beta.-lactam compounds which include an electronegative leaving group. The leaving group comprises a carbamate, carbonate, thiocarbamate or thiocarbonate linkage and a fluorescent moiety or a moiety capable of producing a visually detectable colored product. Upon cleavage of the lactam ring by .beta.-lactamase, the leaving group is liberated and fluorescence or a colored product is produced.Type: GrantFiled: January 30, 1992Date of Patent: August 16, 1994Assignee: Becton, Dickinson and CompanyInventors: J. Michael Quante, Randal A. Hoke, Patrick D. Mize, Daniel L. Woodard, O. Elmo Millner, deceased
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Patent number: 5329000Abstract: A process for purifying DNA in which the DNA is bound to silicon tetrahydrazide in the presence of less than 2M chaotrope, low salt buffers, or water. The DNA is then eluted with low salt buffer or by heating in water.Type: GrantFiled: October 31, 1991Date of Patent: July 12, 1994Assignee: Becton, Dickinson and CompanyInventors: Daniel L. Woodard, Adriann J. Howard