Patents by Inventor Daniel Louis Kacian
Daniel Louis Kacian has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
-
Patent number: 6214587Abstract: Methods for amplifying target nucleic acid sequences using a nucleic acid polymerase lacking 5′ exonuclease activity and a set of oligonucleotide primers. Preferably, a primer array is used. The primer array contains two sets of primers. One set contains at least two complementary primers. The other set contains at least two sense primers. Using the described methods amplification can be carried out under essentially constant environmental conditions without the requirement for exonuclease activity or restriction endonuclease activity.Type: GrantFiled: February 17, 2000Date of Patent: April 10, 2001Assignee: Gen-Probe IncorporatedInventors: Nanibhushan Dattagupta, Paul Douglas Stull, Marc Spingola, Daniel Louis Kacian
-
Patent number: 6087133Abstract: Methods for amplifying target nucleic acid sequences using a nucleic acid polymerase lacking 5' exonuclease activity and a set of oligonucleotide primers. Preferably, a primer array is used. The primer array contains two sets of primers. One set contains at least two complementary primers. The other set contains at least two sense primers. Using the described methods amplification can be carried out under essentially constant environmental conditions without the requirement for exonuclease activity or restriction endonuclease activity.Type: GrantFiled: November 18, 1997Date of Patent: July 11, 2000Assignee: Gen-Probe IncorporatedInventors: Nanibhushan Dattagupta, Paul Douglas Stull, Marc Spingola, Daniel Louis Kacian
-
Patent number: 5998195Abstract: A plasmid for expression of Moloney Murine Leukemia Virus-derived reverse transcriptase in E. coli cells deficient in the expression of indiginous RNAse activity, a method for purification of the recombinant enzyme, and a composition comprising a cloned and purified reverse transcriptase opimized for use in cDNA and nucleic acid amplification procedures.Type: GrantFiled: March 21, 1997Date of Patent: December 7, 1999Assignee: Gen-Probe IncorporatedInventors: Daniel Louis Kacian, Michael Garth Riggs, James Garfield Putnam
-
Patent number: 5935833Abstract: A plasmid for expression of Moloney Murine Leukemia Virus-derived reverse transcriptase in E. coli cells deficient in the expression of indiginous RNAse activity, a method for purification of the recombinant enzyme, and a composition comprising a cloned and purified reverse transcriptase opimized for use in cDNA and nucleic acid amplification procedures.Type: GrantFiled: January 9, 1997Date of Patent: August 10, 1999Assignee: Gen-Probe IncorporatedInventors: Daniel Louis Kacian, Michael Garth Riggs, James Putnam
-
Patent number: 5916777Abstract: The present invention relates to methods of enzymatically synthesizing oligonucleotides. In particular, it relates to the use of 3'-ribonucleotide primers which effectively serve to initiate oligonucleotide synthesis, and can also be easily cleaved from synthesis products and reused in subsequent synthesis reactions.Type: GrantFiled: October 10, 1997Date of Patent: June 29, 1999Assignee: Gen-Probe IncorporatedInventors: Daniel Louis Kacian, Nanibhushan Dattagupta, David Bruce Lackey
-
Patent number: 5888779Abstract: Kits for synthesizing multiple copies of a target nucleic acid sequence autocatalytically under conditions of substantially constant temperature, ionic strength, and pH are provided in which multiple RNA copies of the target sequence autocatalytically generate additional copies. These methods are useful for generating copies of a nucleic acid target sequence for purposes which include assays to quantitate specific nucleic acid sequences in clinical, environmental, forensic and similar samples, cloning and generating probes.Type: GrantFiled: June 5, 1995Date of Patent: March 30, 1999Assignee: Gen-Probe IncorporatedInventors: Daniel Louis Kacian, Timothy J. Fultz
-
Patent number: 5871975Abstract: Methods and kits for the use of a non-ionic detergent to suppress enzyme inhibition in a reaction solution due to the presence of inhibiting ionic detergent. Prior to reaction, the reaction mixure is given an effective amount of a non-ionic detergent, and agitated. The enzyme is then added, and the enzymatic reaction is the allowed to proceed. Also disclosed are preferred embodiments of the present invention, and kits for nucleic acid amplification of a biological sample in the presence of an ionic detergent.Type: GrantFiled: March 25, 1997Date of Patent: February 16, 1999Assignee: Gen-Probe IncorporatedInventors: Daniel Louis Kacian, Diane Lisa McAllister
-
Patent number: 5846701Abstract: Methods and kits for the use of a non-ionic detergent to suppress enzyme inhibition in a reaction solution due to the presence of inhibiting ionic detergent. Prior to reaction, the reaction mixture is given an effective amount of a non-ionic detergent, and agitated. The enzyme is then added, and the enzymatic reaction is the allowed to proceed. Also disclosed are preferred embodiments of the present invention, and kits for nucleic acid amplification of a biological sample in the presence of an ionic detergent.Type: GrantFiled: January 6, 1997Date of Patent: December 8, 1998Assignee: Gen-Probe IncorporatedInventors: Daniel Louis Kacian, Diane Lisa McAllister
-
Patent number: 5834254Abstract: Stabilized enzyme compositions for use in nucleic acid amplification. Compositions are provided for the stabilization of one or more enzymes in a single stabilized formulation. Additional compositions incorporate a dried, stabilized enzyme mixture together with necessary cofactors and enzyme substrates in a single container for use upon rehydration. Also disclosed are methods for making and using stabilized enzyme compositions and kits for nucleic acid amplification incorporating the disclosed compositions.Type: GrantFiled: July 22, 1997Date of Patent: November 10, 1998Assignee: Gen-Probe IncorporatedInventors: Nancy Lau Liu Shen, Daniel Louis Kacian, James Garfield Putnam, William Michael Davis
-
Patent number: 5824518Abstract: Methods of synthesizing multiple copies of a target nucleic acid sequence autocatalytically under conditions of substantially constant temperature, ionic strength, and pH are provided in which multiple RNA copies of the target sequence autocatalytically generate additional copies. These methods are useful for generating copies of a nucleic acid target sequence for purposes which include assays to quantitate specific nucleic acid sequences in clinical, environmental, forensic and similar samples, cloning and generating probes.Type: GrantFiled: June 6, 1995Date of Patent: October 20, 1998Assignee: Gen-Probe IncorporatedInventors: Daniel Louis Kacian, Timothy J. Fultz
-
Patent number: 5766890Abstract: Methods and kits for the use of a non-ionic detergent to suppress enzyme inhibition in a reaction solution due to the presence of inhibiting ionic detergent. Prior to reaction, the reaction mixture is given an effective amount of a non-ionic detergent, and agitated. The enzyme is then added, and the enzymatic reaction is the allowed to proceed. Also disclosed are preferred embodiments of the present invention, and kits for nucleic acid amplification of a biological sample in the presence of an ionic detergent.Type: GrantFiled: January 6, 1997Date of Patent: June 16, 1998Assignee: Gen-Probe IncorporatedInventors: Daniel Louis Kacian, Diane Lisa McAllister
-
Patent number: 5739311Abstract: The present invention relates to methods of synthesizing phosphorothioate oligonucleotides. In particular, it relates to the use of certain restriction endonucleases to cleave phosphorothioate oligonucleotides which contain restriction endonuclease recognition sequences. These restriction sequences facilitate the cleavage of relatively cleavage resistant phosphorothioate oligonucleotides thus facilitating their separation and purification after synthesis.Type: GrantFiled: June 7, 1995Date of Patent: April 14, 1998Assignee: Gen-Probe IncorporatedInventors: David Bruce Lackey, Nanibhushan Dattagupta, Daniel Louis Kacian
-
Patent number: 5710029Abstract: Methods and kits for relating initial amounts of target nucleic acids present in a sample to target-specific amplification products. It has been discovered that the transcription-mediated amplification system is capable of producing a quantitative relationship between target input and target-specific output. Further, the present invention relates to methods for carefully controlling this relationship resulting in an unexpectedly high degree of reproducability. Also described are useful methods for extending the dynamic range of transcription-based amplification systems.Type: GrantFiled: June 7, 1995Date of Patent: January 20, 1998Assignee: Gen-Probe IncorporatedInventors: Thomas Brendan Ryder, Karen W. Shannon, Daniel Louis Kacian, Richard C. Harvey, Sherrol H. McDonough, Frank R. Gonzales, Maria R. Castillo, Elizabeth R. Billyard, Nancy Lau Liu Shen
-
Patent number: 5705365Abstract: Methods and kits for relating initial amounts of target nucleic acids present in a sample to target-specific amplification products. It has been discovered that the transcription-mediated amplification system is capable of producing a quantitative relationship between target input and target-specific output. Further, the the present invention relates to methods for carefully controlling this relationship resulting in an unexpectedly high degree of reproducability. Also described are useful methods for extending the dynamic range of transcription-based amplification systems.Type: GrantFiled: June 7, 1995Date of Patent: January 6, 1998Assignee: Gen-Probe IncorporatedInventors: Thomas Brendan Ryder, Karen W. Shannon, Daniel Louis Kacian, Richard C. Harvey, Sherrol H. McDonough, Frank R. Gonzales, Maria R. Castillo, Elizabeth R. Billyard, Nancy Lau Liu Shen
-
Patent number: 5652126Abstract: The present invention relates to methods of cleaving phosphorothioate oligonucleotides. In particular, it relates to the use of certain restriction endonucleases to cleave phosphorothioate oligonucleotides which contain restriction endonuclease recognition sequences. These restriction sequences can be used to facilitate the cleavage of relatively cleavage resistant phosphorothioate oligonucleotides thus facilitating their separation and purification after synthesis.Type: GrantFiled: June 7, 1995Date of Patent: July 29, 1997Assignee: Gen-Probe IncorporatedInventors: David Bruce Lackey, Nanibhushan Dattagupta, Daniel Louis Kacian
-
Patent number: RE38960Abstract: Methods for amplifying target nucleic acid sequences using a nucleic acid polymerase lacking 5? exonuclease activity and a set of oligonucleotide primers. Preferably, a primer array is used. The primer array contains two sets of primers. One set contains at least two complementary primers. The other set contains at least two sense primers. Using the described methods amplification can be carried out under essentially constant environmental conditions without the requirement for exonuclease activity or restriction endonuclease activity.Type: GrantFiled: June 7, 2002Date of Patent: January 31, 2006Assignee: Gen-Probe IncorporatedInventors: Nanibhushan Dattagupta, Paul Douglas Stull, Marc Spingola, Daniel Louis Kacian
-
Patent number: RE39007Abstract: Methods for amplifying target nucleic acid sequences using a nucleic acid polymerase lacking 5? exonuclease activity and a set of oligonucleotide primers. Preferably, a primer array is used. The primer array contains two sets of primers. One set contains at least two complementary primers. The other set contains at least two sense primers. Using the described methods amplification can be carried out under essentially constant environmental conditions without the requirement for exonuclease activity or restriction endonu- clease activity.Type: GrantFiled: June 7, 2002Date of Patent: March 7, 2006Assignee: Gen-Probe IncorporatedInventors: Nanibhushan Dattagupta, Paul Douglas Stull, Marc Spingola, Daniel Louis Kacian