Patents by Inventor Daniel M. Widmaier

Daniel M. Widmaier has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 12577354
    Abstract: The present disclosure provides methods and compositions for directed to synthetic block copolymer proteins, expression constructs for their secretion, recombinant microorganisms for their production, and synthetic fibers (including advantageously, microfibers) comprising these proteins that recapitulate many properties of natural silk. The recombinant microorganisms can be used for the commercial production of silk-like fibers.
    Type: Grant
    Filed: October 10, 2022
    Date of Patent: March 17, 2026
    Assignee: BOLT THREADS, INC.
    Inventors: Daniel M. Widmaier, David N. Breslauer, Joshua Kittleson, Brendan Turner, Lindsay Wray
  • Publication number: 20250289856
    Abstract: The present disclosure relates to methods for producing recombinant proteins, as well as compositions used in and produced by such methods. Specifically, the present disclosure relates to methods for producing high secreted yields of recombinant proteins, and the compositions provided herein include expression constructs, recombinant vectors, and recombinant host cells that comprise polynucleotide sequences encoding proteins operably linked to recombinant secretion signals that comprise the leader peptide of the ?-mating factor (?MF) of Saccharomyces cerevisiae and a non-?MF signal peptide.
    Type: Application
    Filed: May 30, 2025
    Publication date: September 18, 2025
    Inventors: Joshua Kittleson, Thomas Stevens, Rena Hill, Carlos Gustavo Pesce, David N. Breslauer, Daniel M. Widmaier
  • Patent number: 12325730
    Abstract: The present disclosure relates to methods for producing recombinant proteins, as well as compositions used in and produced by such methods. Specifically, the present disclosure relates to methods for producing high secreted yields of recombinant proteins, and the compositions provided herein include expression constructs, recombinant vectors, and recombinant host cells that comprise polynucleotide sequences encoding proteins operably linked to recombinant secretion signals that comprise the leader peptide of the ?-mating factor (?MF) of Saccharomyces cerevisiae and a non-?MF signal peptide.
    Type: Grant
    Filed: June 26, 2023
    Date of Patent: June 10, 2025
    Assignee: BOLT THREADS, INC.
    Inventors: Joshua Kittleson, Thomas Stevens, Rena Hill, Carlos Gustavo Pesce, David N. Breslauer, Daniel M. Widmaier
  • Publication number: 20240294584
    Abstract: This invention relates to elastomeric protein and elastomeric protein production. In particular, the invention is directed to elastomeric protein sequences, including methods and compositions for production of elastomeric protein sequences, such as expression constructs, and host cells, and including compositions generated from the elastomeric protein sequences.
    Type: Application
    Filed: December 8, 2023
    Publication date: September 5, 2024
    Inventors: Joshua Kittleson, Michael Lee, David N. Breslauer, Daniel M. Widmaier
  • Publication number: 20240150416
    Abstract: The present disclosure relates to methods for producing recombinant proteins, as well as compositions used in and produced by such methods. Specifically, the present disclosure relates to methods for producing high secreted yields of recombinant proteins, and the compositions provided herein include expression constructs, recombinant vectors, and recombinant host cells that comprise polynucleotide sequences encoding proteins operably linked to recombinant secretion signals that comprise the leader peptide of the ?-mating factor (?MF) of Saccharomyces cerevisiae and a non-?MF signal peptide.
    Type: Application
    Filed: June 26, 2023
    Publication date: May 9, 2024
    Inventors: Joshua Kittleson, Thomas Stevens, Rena Hill, Carlos Gustavo Pesce, David N. Breslauer, Daniel M. Widmaier
  • Patent number: 11858971
    Abstract: This invention relates to elastomeric protein and elastomeric protein production. In particular, the invention is directed to elastomeric protein sequences, including methods and compositions for production of elastomeric protein sequences, such as expression constructs, and host cells, and including compositions generated from the elastomeric protein sequences.
    Type: Grant
    Filed: March 31, 2021
    Date of Patent: January 2, 2024
    Assignee: BOLT THREADS, INC.
    Inventors: Joshua Kittleson, Michael Lee, David N. Breslauer, Daniel M. Widmaier
  • Patent number: 11725030
    Abstract: The present disclosure relates to methods for producing recombinant proteins, as well as compositions used in and produced by such methods. Specifically, the present disclosure relates to methods for producing high secreted yields of recombinant proteins, and the compositions provided herein include expression constructs, recombinant vectors, and recombinant host cells that comprise polynucleotide sequences encoding proteins operably linked to recombinant secretion signals that comprise the leader peptide of the ?-mating factor (?MF) of Saccharomyces cerevisiae and a non-?MF signal peptide.
    Type: Grant
    Filed: March 15, 2022
    Date of Patent: August 15, 2023
    Assignee: BOLT THREADS, INC.
    Inventors: Joshua Kittleson, Thomas Stevens, Rena Hill, Carlos Gustavo Pesce, David N. Breslauer, Daniel M. Widmaier
  • Publication number: 20230192965
    Abstract: The present disclosure provides methods and compositions for directed to synthetic block copolymer proteins, expression constructs for their secretion, recombinant microorganisms for their production, and synthetic fibers (including advantageously, microfibers) comprising these proteins that recapitulate many properties of natural silk. The recombinant microorganisms can be used for the commercial production of silk-like fibers.
    Type: Application
    Filed: October 10, 2022
    Publication date: June 22, 2023
    Inventors: Daniel M. Widmaier, David N. Breslauer, Joshua Kittleson, Brendan Turner, Lindsay Wray
  • Patent number: 11505654
    Abstract: The present disclosure provides methods and compositions for directed to synthetic block copolymer proteins, expression constructs for their secretion, recombinant microorganisms for their production, and synthetic fibers (including advantageously, microfibers) comprising these proteins that recapitulate many properties of natural silk. The recombinant microorganisms can be used for the commercial production of silk-like fibers.
    Type: Grant
    Filed: June 22, 2018
    Date of Patent: November 22, 2022
    Assignee: BOLT THREADS, INC.
    Inventors: Daniel M. Widmaier, David N. Breslauer, Joshua Kittleson, Brendan Turner, Lindsay Wray
  • Publication number: 20220315630
    Abstract: The present disclosure relates to methods for producing recombinant proteins, as well as compositions used in and produced by such methods. Specifically, the present disclosure relates to methods for producing high secreted yields of recombinant proteins, and the compositions provided herein include expression constructs, recombinant vectors, and recombinant host cells that comprise polynucleotide sequences encoding proteins operably linked to recombinant secretion signals that comprise the leader peptide of the a-mating factor (?MF) of Saccharomyces cerevisiae and a non-?MF signal peptide.
    Type: Application
    Filed: March 15, 2022
    Publication date: October 6, 2022
    Inventors: Joshua Kittleson, Thomas Stevens, Rena Hill, Carlos Gustavo Pesce, David N. Breslauer, Daniel M. Widmaier
  • Patent number: 11306127
    Abstract: The present disclosure relates to methods for producing recombinant proteins, as well as compositions used in and produced by such methods. Specifically, the present disclosure relates to methods for producing high secreted yields of recombinant proteins, and the compositions provided herein include expression constructs, recombinant vectors, and recombinant host cells that comprise polynucleotide sequences encoding proteins operably linked to recombinant secretion signals that comprise the leader peptide of the ?-mating factor (?MF) of Saccharomyces cerevisiae and a non-?MF signal peptide.
    Type: Grant
    Filed: March 13, 2018
    Date of Patent: April 19, 2022
    Assignee: Bolt Threads, Inc.
    Inventors: Joshua Kittleson, Thomas Stevens, Rena Hill, Carlos Gustavo Pesce, David N. Breslauer, Daniel M. Widmaier
  • Patent number: 11192982
    Abstract: The present disclosure provides methods and compositions for directed to synthetic block copolymer proteins, expression constructs for their secretion, recombinant microorganisms for their production, and synthetic fibers (including advantageously, microfibers) comprising these proteins that recapitulate many properties of natural silk. The recombinant microorganisms can be used for the commercial production of silk-like fibers.
    Type: Grant
    Filed: August 30, 2019
    Date of Patent: December 7, 2021
    Assignee: Bolt Threads, Inc.
    Inventors: Daniel M. Widmaier, David N. Breslauer, Joshua Kittleson, Brendan Turner, Lindsay Wray
  • Publication number: 20210340194
    Abstract: This invention relates to elastomeric protein and elastomeric protein production. In particular, the invention is directed to elastomeric protein sequences, including methods and compositions for production of elastomeric protein sequences, such as expression constructs, and host cells, and including compositions generated from the elastomeric protein sequences.
    Type: Application
    Filed: March 31, 2021
    Publication date: November 4, 2021
    Inventors: Joshua Kittleson, Michael Lee, David N. Breslauer, Daniel M. Widmaier
  • Patent number: 10988515
    Abstract: This invention relates to elastomeric protein and elastomeric protein production. In particular, the invention is directed to elastomeric protein sequences, including methods and compositions for production of elastomeric protein sequences, such as expression constructs, and host cells, and including compositions generated from the elastomeric protein sequences.
    Type: Grant
    Filed: January 16, 2018
    Date of Patent: April 27, 2021
    Assignee: Bolt Threads, Inc.
    Inventors: Joshua Kittleson, Michael Lee, David N. Breslauer, Daniel M. Widmaier
  • Publication number: 20200055996
    Abstract: The present disclosure provides methods and compositions for directed to synthetic block copolymer proteins, expression constructs for their secretion, recombinant microorganisms for their production, and synthetic fibers (including advantageously, microfibers) comprising these proteins that recapitulate many properties of natural silk. The recombinant microorganisms can be used for the commercial production of silk-like fibers.
    Type: Application
    Filed: August 30, 2019
    Publication date: February 20, 2020
    Inventors: Daniel M. Widmaier, David N. Breslauer, Joshua Kittleson, Brendan Turner, Lindsay Wray
  • Publication number: 20190330287
    Abstract: This invention relates to elastomeric protein and elastomeric protein production. In particular, the invention is directed to elastomeric protein sequences, including methods and compositions for production of elastomeric protein sequences, such as expression constructs, and host cells, and including compositions generated from the elastomeric protein sequences.
    Type: Application
    Filed: January 16, 2018
    Publication date: October 31, 2019
    Inventors: Joshua Kittleson, Michael Lee, David N. Breslauer, Daniel M. Widmaier
  • Patent number: 10435516
    Abstract: The present disclosure provides methods and compositions for directed to synthetic block copolymer proteins, expression constructs for their secretion, recombinant microorganisms for their production, and synthetic fibers (including advantageously, microfibers) comprising these proteins that recapitulate many properties of natural silk. The recombinant microorganisms can be used for the commercial production of silk-like fibers.
    Type: Grant
    Filed: April 4, 2018
    Date of Patent: October 8, 2019
    Assignee: Bolt Threads, Inc.
    Inventors: Daniel M. Widmaier, David N. Breslauer, Joshua Kittleson, Brendan Turner, Lindsay Wray
  • Publication number: 20180298151
    Abstract: The present disclosure provides methods and compositions for directed to synthetic block copolymer proteins, expression constructs for their secretion, recombinant microorganisms for their production, and synthetic fibers (including advantageously, microfibers) comprising these proteins that recapitulate many properties of natural silk. The recombinant microorganisms can be used for the commercial production of silk-like fibers.
    Type: Application
    Filed: June 22, 2018
    Publication date: October 18, 2018
    Inventors: Daniel M. Widmaier, David N. Breslauer, Joshua Kittleson, Brendan Turner, Lindsay Wray
  • Publication number: 20180282380
    Abstract: The present disclosure relates to methods for producing recombinant proteins, as well as compositions used in and produced by such methods. Specifically, the present disclosure relates to methods for producing high secreted yields of recombinant proteins, and the compositions provided herein include expression constructs, recombinant vectors, and recombinant host cells that comprise polynucleotide sequences encoding proteins operably linked to recombinant secretion signals that comprise the leader peptide of the ?-mating factor (?MF) of Saccharomyces cerevisiae and a non-?MF signal peptide.
    Type: Application
    Filed: March 13, 2018
    Publication date: October 4, 2018
    Inventors: Joshua Kittleson, Thomas Stevens, Rena Hill, Carlos Gustavo Pesce, David N. Breslauer, Daniel M. Widmaier
  • Publication number: 20180273693
    Abstract: The present disclosure provides methods and compositions for directed to synthetic block copolymer proteins, expression constructs for their secretion, recombinant microorganisms for their production, and synthetic fibers (including advantageously, microfibers) comprising these proteins that recapitulate many properties of natural silk. The recombinant microorganisms can be used for the commercial production of silk-like fibers.
    Type: Application
    Filed: April 4, 2018
    Publication date: September 27, 2018
    Inventors: Daniel M. Widmaier, David N. Breslauer, Joshua Kittleson, Brendan Turner, Lindsay Wray