Abstract: A method images flow cytometry by moving a sample along an axis of movement, and generating recorded images of the sample. Each recorded image is generated by illuminating the sample by illumination light, forming an image of the sample by collecting detection light originating from or interacting with the sample, and generating a recorded image of the sample by recording the image of the sample during its movement. A combined image of the sample is then generated by selecting at least two recorded images, generating a transformed recorded image for each selected recorded image, and combining the transformed recorded images into a combined image. The transformed recorded image is generated by: extracting at least part of the selected recorded image, and applying a transformation to the extracted part of the selected recorded image.

Abstract: A method for acquiring 2D images of a sample includes illuminating the sample with an illumination beam forming a light sheet, and detecting light emitted from the sample along two different imaging paths by using a first imaging objective and a second imaging objective, respectively. The method further includes obtaining a first stack of images using the first imaging objective by sequentially illuminating the sample by adjusting the light sheet according to a first adjustment setting, and obtaining a second stack of images using the second imaging objective by sequentially illuminating the sample by adjusting the light sheet according a second adjustment setting. Each of the first stack and the second stack of images represents multiple planes of the sample in a z-direction. The first stack of images and the second stack of images are characterized by a shift of the planes in the z-direction relative to each other.

Abstract: The invention discloses a microfluidic device for the culture, selection and/or analysis of sample organisms such as nematodes, as well as for other biological entities such as for instance animal embryos. The device features reservoirs, culture chambers and smart filtering systems allowing for the selection of specific populations/specimens of sample organisms, thus permitting long-term cultures thereof as well as phenotypic/behavioural analyses. Systems and methods for using the microfluidic device are within the present disclosure as well.

Abstract: The invention discloses a microfluidic device for the culture, selection and/or analysis of sample organisms such as nematodes, as well as for other biological entities such as for instance animal embryos. The device features reservoirs, culture chambers and smart filtering systems allowing fir the selection of specific populations/specimens of sample organisms, thus permitting long-term cultures thereof as well as phenotypic/behavioural analyses. Systems and methods for using the microfluidic device are within the present disclosure as well.

Abstract: The invention discloses a microfluidic device for the culture, selection and/or analysis of sample organisms such as nematodes, as well as for other biological entities such as for instance animal embryos. The device features reservoirs, culture chambers and smart filtering systems allowing for the selection of specific populations/specimens of sample organisms, thus permitting long-term cultures thereof as well as phenotypic/behavioural analyses. Systems and methods for using the microfluidic device are within the present disclosure as well.

Abstract: The invention discloses a microfluidic device for the culture, selection and/or analysis of sample organisms such as nematodes, as well as for other biological entities such as for instance animal embryos. The device features reservoirs, culture chambers and smart filtering systems allowing for the selection of specific populations/specimens of sample organisms, thus permitting long-term cultures thereof as well as phenotypic/behavioural analyses. Systems and methods for using the microfluidic device are within the present disclosure as well.