Abstract: This disclosure relates to novel PMS1 targeting sequences. Novel PMS1 targeting oligonucleotides for the treatment of trinucleotide repeat disease or disorder are also provided.

Abstract: This disclosure relates to novel PMS2 targeting sequences. Novel PMS2 targeting oligonucleotides for the treatment of a trinucleotide repeat disease or disorder are also provided.

Abstract: This disclosure relates to novel ATN1 targeting sequences. Novel ATN1 targeting oligonucleotides for the treatment of neurodegenerative diseases are also provided.

Abstract: This disclosure relates to novel modified oligonucleotides with increased stability and extended in vivo mRNA silencing activity.

Abstract: This disclosure relates to novel MLH1 targeting sequences. Novel MLH1 targeting oligonucleotides for the treatment of neurodegenerative diseases are also provided.

Abstract: This disclosure relates to novel MLH3 targeting sequences. Novel MLH3 targeting oligonucleotides for the treatment of neurodegenerative diseases are also provided.

Abstract: There is provided herein, processes for synthesizing oligonucleotides using mechanochemical force to induce the formation of internucleotide linkages.

Abstract: This disclosure relates to novel SARS-CoV-2 targeting sequences. Novel SARS-CoV-2 targeting oligonucleotides for the treatment of SARS-CoV-2 infection are also provided.

Abstract: An aircraft air quality testing system includes a portable housing unit to be removably placed within an aircraft; an air testing unit secured inside the portable housing unit and including: a sensor to receive an air sample during a plurality of oxygen generation system cycles and engine thrust settings of the aircraft; a removable collection media to receive the air sample from the sensor and filter targeted chemicals from the air sample; and a plurality of analyzers to perform a real-time chemical analysis of the air sample and the filtered targeted chemicals. A first computer is operatively connected to the portable housing unit and includes: a processor to receive the real-time chemical analysis and generate real-time chemical analysis and flow rate data; a memory device to store the real-time chemical analysis and flow rate data; and a display device operatively connected to the portable housing unit.

Abstract: A self-timing, passive, chemical dosimeter. The dosimeter includes a sampling media and electronics supported by a cassette. The sampling media is configured to absorb volatile organic compounds, semivolatile organic compounds, or both, while the electronics are configured to record a time of exposure. The dosimeter further includes an actuator having a closed position and an open position. In the closed position, the actuator resists exposure of the sampling media to volatile organic compounds, semivolatile organic compounds, or both, and in the open position, the actuator permits exposure of the sampling media to volatile organic compounds, semivolatile organic compounds, or both. The actuator is configured to operate the electronics for recording time of exposure.

Abstract: This disclosure relates to novel HTT-1A targeting sequences. Novel HTT-1A targeting oligonucleotides for the treatment of neurodegenerative diseases are also provided.

Abstract: A CRISPR inhibitor molecule is provided, comprising an artificial nucleic acid construct having a first polynucleotide, the inhibitor molecule capable of establishing several points of contact with a CRISPR protein and high binding affinity thereto, is provided. The first polynucleotide may comprise a sequence selected from the group consisting of: a polynucleotide that interacts with a protospacer adjacent motif (PAM)-interaction (PI) domain of a CRISPR-associated (Cas) protein, a polynucleotide that interacts with a guide sequence of a crRNA or an equivalent position of a single-guide RNA, and a polynucleotide that interacts with a repeat region of a tracrRNA or an equivalent position of a single-guide RNA. The CRISPR inhibitor molecule may also comprise a second polynucleotide and a linker. Methods of using the CRISPR inhibitor molecule in therapeutic agent selection and creation, as well as part of a therapeutic treatment, are also provided.

Abstract: The present disclosure provides CRISPR/Cas9 ribonucleoprotein compositions comprising chemically modified CRISPR RNA (crRNA) guide and trans-acting CRISPR RNA (tracrRNA) components. Methods of using the disclosed CRISPR/Cas9 ribonucleoprotein compositions are also provided.

Abstract: A tool for in-situ airfoil contouring is provided. The tool has a pathway for an airfoil edge wherein the edge movement is limited and further wherein the edge is exposed to a grinding structure having a preselected profile shape therein. The tool makes this contouring process available for airfoils without the necessity of removing the airfoils from, for example, an aircraft engine.

Abstract: A tool for in-situ airfoil contouring is provided. The tool has a pathway for an airfoil edge wherein the edge movement is limited and further wherein the edge is exposed to a grinding structure having a preselected profile shape therein. The tool makes this contouring process available for airfoils without the necessity of removing the airfoils from, for example, an aircraft engine.

Abstract: A system for determining changes in the interest of a subject over a time period. The system measures the volume of news articles for a subject over a time period and makes a comparison to a previous volume of news articles for the same subject over a previous time period.

Abstract: A LAN/WAN network topology and design methodology using Internet Protocol (IP) subnet topology, ATM WAN configuration, equipment placement, and device configuration to provide partitioning of a call processing application across multiple sites. The partitioning reduces latency for mission critical messages, while providing for necessary provisioning traffic needs. Further, the overall topology provides the redundancy and resiliency necessary for mission critical call processing application, utilizing the IP subnets, ATM permanent virtual circuits, network device configuration, and server segregation to achieve Quality of Service (QoS).