Abstract: Provided are systems, kits, and methods for the quantitative detection of single nucleotide polymorphisms or variants to identify malignant neoplasms. The methods include use of modified oligonucleotide blockers with peptide nucleic acid backbones that hybridize to and block logarithmic amplification of the wild-type alleles of a target, and incorporation of locked nucleic acids into probes that are complementary to a mutant allele of the target sequence to increase specificity. The methods include detection of variants in sequences with high GC content and/or low complexity, such as the TERT promoter, IDH1, BRAF, NRAS, GNAQ, GNA11 and H3F3 A gene variants. The methods include sensitive detection and staging of cancers with low cellularity, and can be used intraoperatively such as for glioma, or to detect cell-free circulating tumor DNA, such as for melanoma.

Abstract: Provided are systems, kits, and methods for the quantitative detection of single nucleotide polymorphisms or variants to identify malignant neoplasms. The methods include use of modified oligonucleotide blockers with peptide nucleic acid backbones that hybridize to and block logarithmic amplification of the wild-type alleles of a target, and incorporation of locked nucleic acids into probes that are complementary to a mutant allele of the target sequence to increase specificity. The methods include detection of variants in sequences with high GC content and/or low complexity, such as the TERT promoter, IDH1, BRAF, NRAS, GNAQ, GNA11 and H3F3 A gene variants. The methods include sensitive detection and staging of cancers with low cellularity, and can be used intraoperatively such as for glioma, or to detect cell-free circulating tumor DNA, such as for melanoma.

Abstract: The present application is directed to sensors and methods of use to determine a width of a media sheet moving along a media path. In one embodiment, the sensor includes a shaft that extends at least partially across the media path. First and second paddles may extend outward from the shaft and into the media path. The paddles may be axially spaced apart along a length of the shaft, and the first paddle may be positioned upstream along the media path from the second paddle. A flag may extend outward from the shaft. A detector may be positioned in proximity to the shaft. In use, the shaft may rotate during contact between the media sheets and the paddles to move the flag. The detector may be able to differentiate between a first amount of rotation due to contact with a wide media sheet and a second amount of rotation with a narrow media sheet to determine a width of the media sheets.

Abstract: The present application is directed to sensors and methods of use to determine a width of a media sheet moving along a media path. In one embodiment, the sensor includes a shaft that extends at least partially across the media path. First and second paddles may extend outward from the shaft and into the media path. The paddles may be axially spaced apart along a length of the shaft, and the first paddle may be positioned upstream along the media path from the second paddle. A flag may extend outward from the shaft. A detector may be positioned in proximity to the shaft. In use, the shaft may rotate during contact between the media sheets and the paddles to move the flag. The detector may be able to differentiate between a first amount of rotation due to contact with a wide media sheet and a second amount of rotation with a narrow media sheet to determine a width of the media sheets.

Abstract: The present application is directed to methods of determining a width of a media sheet moving along a media path of an image forming apparatus. The methods may include moving the media sheet along the media path and past a media width sensor that includes a shaft with a first upstream paddle positioned away from a reference location of the media path and a second downstream paddle positioned in closer proximity to the reference location. The first paddle is positioned a first distance from the first sensor, and the second paddle is positioned a second distance from the first sensor. As the media sheet moves along the media path, a first signal may be received when the media sheet contacts the first sensor, and a second signal when the media sheet is at the media width sensor. The distance the media sheet travels between contacting the first sensor and being at the second sensor may be determined.

Abstract: A device and method for applying images to continuous media stock, such as rolls of paper, blank labels, etc., using a cut-sheet image forming device. While the continuous media stock moves along a media path in the cut-sheet image forming device, one or more sensors in the cut-sheet image forming device detect inter-sheet holes disposed along the continuous media stock. Based on the detected inter-sheet holes, the cut-sheet image forming device tracks the position of the continuous media stock. By tracking the position of the continuous media stock, the cut-sheet image forming device can identify specific sections of the continuous media stock as image areas appropriate for image transfer.

Abstract: An improved electrophotographic printer is provided for printing on sheet media, and for joining two or more sheets of sheet media together within the print engine. A first sheet can be imaged with “standard” information and/or indicia, and can also have one or more strips of toner applied at predetermined locations. The first sheet is then run back to the print engine while a second (virgin) sheet of print media is picked from an input tray at a time that will cause both sheets to run through the print engine simultaneously, either exactly at the same time, or with some offset spacing. The two sheets will become joined or affixed to one another at locations in which the toner strips are of sufficient size and density, and will be output together as if stapled, or sealed, together.