Patents by Inventor Daniel R. Demarco

Daniel R. Demarco has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 10000819
    Abstract: This invention relates to a rapid method for detection and characterization of Escherichia coli bacteria serotype O157:H7 based on the presence of nucleic acid sequences, in particular, to a PCR-based method for detection, and to oligonucleotide molecules and reagents and kits useful therefore. This method is preferably employed to detect E. coli O157:H7 in a food or water sample, such as a beef enrichment. The present invention further relates to replication compositions and kits for carrying out the method of the present invention.
    Type: Grant
    Filed: February 18, 2014
    Date of Patent: June 19, 2018
    Assignee: Qualicon Diagnostics LLC
    Inventors: Daniel R. Demarco, Mark A. Jensen, Stephen Varkey
  • Patent number: 9528162
    Abstract: This invention relates to a rapid method for detection and characterization of STEC bacteria based on the presence of nucleic acid sequences, in particular, to a PCR-based method for detection, and to oligonucleotide molecules and reagents and kits useful therefore. This method is preferably employed to detect STEC bacteria in a food or water sample, such as a beef enrichment. The present invention further relates to isolated polynucleotides, replication compositions, kits, and reagent tablets for carrying out the method of the present invention.
    Type: Grant
    Filed: July 8, 2014
    Date of Patent: December 27, 2016
    Assignee: E I DU PONT DE NEMOURS AND COMPANY
    Inventors: Stephen Varkey, Daniel R. DeMarco, Mark A. Jensen
  • Publication number: 20160201116
    Abstract: This invention relates to a rapid, accurate method for detection and characterization of Salmonella enteritidis and/or Salmonella typhimurium based on the presence of nucleic acid sequences, in particular, to a PCR-based method for detection, and to oligonucleotide molecules and reagents and kits useful therefore. This method is preferably employed to detect S. enteritidis and/or S. typhimurium in an environmental sample. The present invention further relates to replication compositions and kits for carrying out the method of the present invention.
    Type: Application
    Filed: August 19, 2014
    Publication date: July 14, 2016
    Inventors: Mark A. Jensen, Daniel R. Demarco, Stephen Varkey, Jun Li
  • Patent number: 9388473
    Abstract: This invention relates to a rapid method for detection of Salmonella in a sample based on the presence of nucleic acid sequences, in particular, to a PCR-based method for detection, and to oligonucleotide molecules and reagents and kits useful therefore. In certain embodiments, the method is employed to detect Salmonella in a food or water sample. The present invention further relates to isolated polynucleotides, replication compositions, and kits for carrying out the method of the present invention.
    Type: Grant
    Filed: November 7, 2012
    Date of Patent: July 12, 2016
    Assignee: E I DU PONT DE NEMOURS AND COMPANY
    Inventors: Stephen Varkey, Daniel R. DeMarco
  • Publication number: 20150376686
    Abstract: This invention relates to a rapid method for detection and characterization of Escherichia coli bacteria serotype O157:H7 based on the presence of nucleic acid sequences, in particular, to a PCR-based method for detection, and to oligonucleotide molecules and reagents and kits useful therefore. This method is preferably employed to detect E. coli O157:H7 in a food or water sample, such as a beef enrichment. The present invention further relates to replication compositions and kits for carrying out the method of the present invention.
    Type: Application
    Filed: February 18, 2014
    Publication date: December 31, 2015
    Applicant: E.I. DU PONT DE NEMOURS AND COMPANY
    Inventors: Daniel R. Demarco, Mark A. Jensen, Stephen Varkey
  • Publication number: 20140323710
    Abstract: This invention relates to a rapid method for detection and characterization of STEC bacteria based on the presence of nucleic acid sequences, in particular, to a PCR-based method for detection, and to oligonucleotide molecules and reagents and kits useful therefore. This method is preferably employed to detect STEC bacteria in a food or water sample, such as a beef enrichment. The present invention further relates to isolated polynucleotides, replication compositions, kits, and reagent tablets for carrying out the method of the present invention.
    Type: Application
    Filed: July 8, 2014
    Publication date: October 30, 2014
    Inventors: Stephen Varkey, Daniel R. DeMarco, Mark A. Jensen
  • Patent number: 8802371
    Abstract: This invention relates to a rapid method for detection and characterization of STEC bacteria based on the presence of nucleic acid sequences, in particular, to a PCR-based method for detection, and to oligonucleotide molecules and reagents and kits useful therefore. This method is preferably employed to detect STEC bacteria in a food or water sample, such as a beef enrichment. The present invention further relates to isolated polynucleotides, replication compositions, kits, and reagent tablets for carrying out the method of the present invention.
    Type: Grant
    Filed: September 28, 2012
    Date of Patent: August 12, 2014
    Assignee: E. I. du Pont de Nemours and Company
    Inventors: Stephen Varkey, Daniel R. DeMarco, Mark A. Jensen
  • Publication number: 20130078634
    Abstract: This invention relates to a rapid method for detection and characterization of STEC bacteria based on the presence of nucleic acid sequences, in particular, to a PCR-based method for detection, and to oligonucleotide molecules and reagents and kits useful therefore. This method is preferably employed to detect STEC bacteria in a food or water sample, such as a beef enrichment. The present invention further relates to isolated polynucleotides, replication compositions, kits, and reagent tablets for carrying out the method of the present invention.
    Type: Application
    Filed: September 28, 2012
    Publication date: March 28, 2013
    Inventors: Stephen Varkey, Daniel R. DeMarco, Mark A. Jensen
  • Publication number: 20110020794
    Abstract: Methods for the direct detection of diagnostic target RNA have been developed, which obviate the need for time consuming RNA purification and isolation procedures.
    Type: Application
    Filed: July 13, 2009
    Publication date: January 27, 2011
    Applicant: E. I. DU PONT DE NEMOURS AND COMPANY
    Inventors: LAURA A. BEDZYK, Daniel R. Demarco, Richard C. Ebersole, Raymond E. Jackson, Stephen Varkey, Rick Ye
  • Publication number: 20040134795
    Abstract: This invention pertains to the protection of metals from corrosion and/or fouling from exposure to environments such as, for example, seawater. The protection in accordance with this invention is achieved through the use of conductive zinc-containing coatings in combination with applied current cathodic protection.
    Type: Application
    Filed: July 23, 2003
    Publication date: July 15, 2004
    Inventors: Daniel R. Demarco, Basil V. Gregorovich, Robert C. Nahas, Shi Hua Zhang