Abstract: Breath liquid particles and vapor are captured in a device presenting a surface and chamber space that condenses or freezes the vapor and aerosol particulates. One or more breaths are exhaled through the device. Capture can be performed on the freezing surface immobilizing water upon contact. The chamber space within the device may freeze or condense liquid breath particles and vapor to collect them. After collection, the liquid is gathered and collected either by draining, scraping, pushing, or centrifugal force. The liquid may be collected and combined with a sample preparation reagent such as a virus lysing reagent, an internal standard, etc. After collection, the sample is separated into a plurality of independently analyzable units for detection and quantification of a target. Analysis may be performed by PCR, qPCR RT-PCR, RT-qPCR, LAMP, RPA or any nucleic acid detection method, lateral antigen mass spectrometry, spectrophotometry or any analytical tool or method.

Abstract: Breath liquid particles and vapor are captured in a device presenting a surface and chamber space that condenses or freezes the vapor and aerosol particulates. One or more breaths are exhaled through the device. Capture can be performed on the freezing surface immobilizing water upon contact. The chamber space within the device may freeze or condense liquid breath particles and vapor to collect them. After collection, the liquid is gathered and collected either by draining, scraping, pushing, or centrifugal force. The liquid may be collected and combined with a sample preparation reagent such as a virus lysing reagent, an internal standard, etc. After collection, the sample is analyzed. Analysis may be performed by PCR, qPCR RT-PCR, RT-qPCR, LAMP, RPA or any nucleic acid detection method, lateral antigen mass spectrometry, spectrophotometry or any analytical tool or method. Nucleic acid amplification reagents may contain a lysing reagent such as acetonitrile.

Abstract: Breath liquid particles and vapor are captured in a device presenting a surface and chamber space that condenses or freezes the vapor and aerosol particulates. One or more breaths are exhaled through the device. Capture can be performed on the freezing surface immobilizing water upon contact. The chamber space within the device may freeze liquid breath particles and vapor to collect them. After collection, the liquid is gathered and collected either by draining, pushing, or centrifugal force into a vial. The liquid may be collected and combined with a sample preparation reagent such as a virus lysing reagent, an internal standard, etc. After collection, the sample is analyzed. Analysis may be performed by PCR, qPCR RT-PCR, RT-qPCR, LAMP or any nucleic acid detection method, mass spectrometry, spectrophotometry or any analytical tool or method. Nucleic acid amplification reagents may contain a lysing reagent such as acetonitrile.