Abstract: The present invention is directed to a fluorescence polarization assay for opiate alkaloids and their metabolites, to the various components needed for preparing and carrying out such an assay and to methods of making these components. Specifically, tracers, immunogens and antibodies are disclosed, as well as methods for making them. The tracers and the immunogens are made from substituted opiate alkaloids. A fluorescein moiety is included in the tracers, while a poly(amino acid) forms a part of the immunogens. The assay is conducted by measuring the degree of polarization retention of the fluorescence resulting when a sample mixed with antiserum and tracer is irradiated with plane-polarized light.

Abstract: The present invention is directed to a fluorescence polarization assay for opiate alkaloids and their metabolites, to the various components needed for preparing and carrying out such an assay and to methods of making these components. Specifically, tracers, immunogens and antibodies are disclosed, as well as methods for making them. The tracers and the immunogens are made from substituted opiate alkaloids. A fluorescein moiety is included in the tracers, while a poly(amino acid) forms a part of the immunogens. The assay is conducted by measuring the degree of polarization retention of the fluorescence resulting when a sample mixed with antiserum and tracer is irradiated with plane-polarized light.

Abstract: Novel substrates which are amino acid 4-amino phenazones for detecting enzymes, particularly L-gamma-glutamyltransferase, are described. The amino acid gamma-glutamyl-4-aminophenazone reacts with the enzyme to produce 4-aminophenazone which is an amino dye intermediate. The amino dye intermediate is coupled with a second dye intermediate to form a chromogen. The 4-aminophenazone is particularly reacted with a phenolic naphtholic or aniline compound, particularly the phenolic compound 2-hydroxy-3,5-dichlorobenzenesulfonate, to form a red chromogen in the presence of an oxidizing agent, such as bilirubin oxidase.

Abstract: Novel substrates which are amino acid 4-amino phenazones for detecting enzymes, particularly L-gamma-glutamyltransferase, are described. The amino acid gamma-glutamyl-4-aminophenazone reacts with the enzyme to produce 4-aminophenazone which is an amino dye intermediate. The amino dye intermediate is coupled with a second dye intermediate to form a chromogen. The 4-aminophenazone is particularly reacted with a phenolic naphtholic or aniline compound, particularly the phenolic compound 2-hydroxy-3,5-dichlorobenzenesulfonate, to form a red chromogen in the presence of an oxidizing agent, such as bilrubin oxidase.

Abstract: The present invention is directed to a fluorescence polarization assay for opiate alkaloids and their metabolites, to the various components needed for preparing and carrying out such an assay and to methods of making these components. Specifically, tracers, immunogens and antibodies are disclosed, as well as methods for making them. The tracers and the immunogens are made from substituted opiate alkaloids. A fluorescein moiety is included in the tracers, while a poly(amino acid) forms a part of the immunogens. The assay is conducted by measuring the degree of polarization retention of the fluorescence resulting when a sample mixed with antiserum and tracer is irradiated with plane-polarized light.

Abstract: A catalyst and process are provided for producing polyurethanes by contacting an organic polyol and an organic isocyanate with a catalyst comprising an N-substituted alkoxyalkyl piperazine and preferably additionally comprising an N-alkoxyalkyl morpholine wherein the alkylene moieties of the morpholine and piperazine are independently selected from the group consisting of C.sub.1 to about C.sub.5 alkylene moieties, and wherein the alkoxy moieties are independently selected from the group consisting of C.sub.1 to about C.sub.3 alkoxy moieties.

Abstract: A catalyst system and process are provided for producing polyurethane foams using a carboxylic acid blowing agent, wherein an organic polyol and an organic polyisocyanate are contacted with a catalyst system comprising a dialkylcyclohexylamine and an N-substituted alkoxyalkyl piperazine.

Abstract: Polyurethane foams with excellent physical properties are obtained by using a catalyst composition of a tertiary amine and 2-ethylhexanoic acid.

Abstract: New ditertiary aminoalcohols are useful as catalysts in the preparation of polyurethane foams. The new catalysts are particularly stable in the presence of polyols and other components often used in the conjunction with such polyols.

Abstract: Polyurethane foams with excellent physical properties can be obtained by using a partial salt between N,N-dimethylaminopropane-1,3-diamine (DMAPA) and a branched octanoic acid.

Abstract: Polyurethane foams with excellent physical properties can be obtained by using a partial salt between N,N-dimethylaminopropane-1,3-diamine (DMAPA) and phenol.

Abstract: Polyurethane foams with excellent physical properties can be obtained by using a partial salt between N,N-dimethylaminopropane-1,3-diamine (DMAPA) and a branched octanoic acid.

Abstract: Polyurethane foams with excellent physical properties can be obtained by using a partial salt between N,N-dimethylaminopropane-1,3-diamine (DMAPA) and phenol.

Abstract: Polyurethanes based on a resole-type polyol can be significantly improved in their friability when the urethane catalyst is a fully neutralized tertiary amine salt and butyl benzyl phthalate is used as the plasticizer.The polyol is prepared by reacting a phenol and formaldehyde under substantially anhydrous conditions with continuous removal of water, and has a water content of no more than about 5%.

Abstract: The use of catalytic amounts of tris(3-dimethylaminopropyl)amine promotes the formation of urethane linkages. Its unusual activity and properties make this compound very useful in the manufacture of rigid polyurethanes.

Abstract: An excellent catalyst for polyurethane foams is a combination of 1, 3, 5-tris(3-dimethyl-aminopropyl)-s-hexahydrotriazine and 2-(dimethylamino)-ethanol. It has unexpectedly been found that this catalyst combination produces both excellent gelation and blowing reactions.

Abstract: The use of catalytic amounts of pentamethyldipropylenetriamine promotes the formation of urethane links. Its unusual activity and properties makes the new compound very useful in the manufacture of flexible and rigid polyurethanes.