Abstract: The invention relates to a light-field microscope and a light-field microscopy image capturing method, including the steps of providing an illumination radiation from at least one light source; modulating the illumination radiation by virtue of adjusting the intensity distribution thereof; generating a dynamic light sheet in a sample space, in which a sample to be imaged can be arranged; scanning a region of the sample to be imaged using the light sheet; collecting a detection radiation and detecting image signals using light-field technology. The illumination radiation is modulated by virtue of the light source being controlled and an intensity of the illumination radiation provided by the light source being altered, especially in a controlled fashion over time. Image recordings of the sample are triggered for different positions of the light sheet.

Abstract: A device for light field microscopy, comprising: a two-dimensionally spatially resolving detector for detecting light radiated by a sample; a detection beam path having at least one microscope objective lens; and at least one multi-lens array for imaging the light radiated by the sample onto the detector, wherein the multi-lens array is arranged in a plane which is optically conjugated with respect to a rear focal plane of the microscope objective lens, or in the vicinity of such a plane; and a control and evaluation unit for activating the detector and for evaluating the measurement data from the detector. The device also includes an adjustable relay optical system in order to image the rear focal plane of the microscope objective lens into the plane in which the multi-lens array is arranged, or in the vicinity of this plane. A method for light field microscopy is also provided.

Abstract: A method and apparatus for light field microscopy, wherein the following method steps are performed: a) measuring an image data record of a sample using a light field arrangement; b) creating at least one partial data record from the image data record; c) reconstructing a three-dimensional object from the partial data record created in step b).

Abstract: A microscope having a light source for transmitting excitation light, an illumination beam path for guiding the excitation light into a sample region and for modifying a polarization state of the excitation light, a phase plate for creating an illumination pattern, a cylindrical optics unit for creating an elongate distribution of the excitation light, a scanning unit for scanning the elongate distribution of the excitation light through the sample region, a camera for recording images, a detection beam path with a microscope objective for guiding emission light onto the camera and a control unit for controlling the scanning unit and/or the camera and for reading out measurement data from the camera. The camera is arranged in a non-descanned part of the detection beam path and the control unit is configured to synchronize a location of a slot-shaped readout region in a sensor plane of the camera with a location of the elongate distribution of the excitation light in a plane of the sample.

Abstract: Microscope with a linear illumination allows a fast image capture. The resolving power can be increased both transversely to the line by way of what is known as rescanning downstream of the confocal stop and along the line by way of illumination that is laterally structured also in this direction. Moreover, an increase in the resolving power in the direction of the optical axis should be made possible. To this end, the illumination beam path contains a beam shaper for creating a distribution of the illumination light in the sample space, said distribution being intensity-modulated along the optical axis and having a plurality of at least local intensity maxima along the optical axis.

Abstract: A microscope having a light source for transmitting excitation light, an illumination beam path comprising a cylindrical optics unit for shaping the excitation light to form an illumination line and a scanning unit for linearly scanning the sample, a detection beam path for guiding emission light radiated by the sample onto a camera for recording images of the sample, and a control unit for controlling at least the scanning unit and/or the camera and for evaluating measurement data from the camera, the control unit being configured to synchronize respective readout regions on a sensor surface of the camera with a position of the excitation light in a sample region. For varying an axial pose of a plane in the sample optically conjugate to a sensor surface of the camera, the detection beam path comprises a controllable optics unit with variable refractive power, which is effective for the entire emission light that has propagated in the detection beam path to the camera.

Abstract: An apparatus includes a detection beam path, along which detection radiation is guided, and an dichroic beam splitter configured for splitting the detection radiation between first and second detection paths, with a detector being in each detection path. A microlens array is arranged upstream of at least one detector. The first detector has a first spatial resolution, and the second detector has a second spatial resolution that is lower than the first spatial resolution. Also, the first detector has a first temporal resolution, and the second detector has a second temporal resolution that is higher than the first temporal resolution. Captured image data and computationally combined to form a three-dimensionally resolved resulting image.

Abstract: A microscope with fast quasi-confocal detection has a main beam splitter and an adjustable beam deflection unit for moving an illumination light through a sample space. The beam deflection unit is optically arranged between a light source and a main beam splitter such that the sample light away from the beam deflection unit passes to a sensor, wherein both the illumination light and the sample light pass through the same intermediate image.

Abstract: A microscope comprising an illumination beam path for guiding excitation light onto and/or into a sample a detection unit for detecting light emitted from the sample, and a detection beam path with a microscope objective for guiding at least a portion of the light emitted from the sample to the detection unit. In the detection unit, a first detection channel with at least one first detector and at least one further detection channel are formed, with each further detection channel comprising at least one detector. The detection unit includes at least one beam splitter in each case for guiding a portion of the light emitted from the sample into a respective further detection channel. At least one of the detection channels includes a manipulation device for axial displacement of a focal region in order to image axially spaced sample planes in at least two detection channels.

Abstract: An apparatus includes a detection beam path, along which detection radiation is guided, and a means for splitting the detection radiation between first and second detection paths, with a detector being in each detection path. A microlens array is arranged upstream of at least one detector. The first detector has a first spatial resolution, and the second detector has a second spatial resolution that is lower than the first spatial resolution. Also, the first detector has a first temporal resolution, and the second detector has a second temporal resolution that is higher than the first temporal resolution. Captured image data and computationally combined to form a three-dimensionally resolved resulting image.

Abstract: A device and a method for multi-spot scanning microscopy using a segmented color separator are disclosed, wherein a beam splitter is segmented laterally to a surface normal of the beam splitter into at least two filter fields for the selection of wavelength ranges and/or polarization directions. Individual partial illumination beams are thus each guided in an illumination beam path into a light spot on or in a simple to be examined and scanned over it. Detection light, which the sample emits in partial detection means after irradiation using the individual partial illumination beams, is guided onto a detection unit and detected thereby, wherein the partial illumination beams and/or the partial detection beams are each selected according to wavelength ranges and/or polarization directions by means of the beam splitter segmented laterally to a surface normal.

Abstract: A microscope comprising an illumination beam path comprising an illumination control device and an illumination objective for illuminating and scanning a sample with excitation light, a detection beam path comprising a microscope objective for guiding emission light emitted by the sample in the direction of a camera for recording images of the sample, and a control unit for controlling the illumination control device and the camera. The control unit being configured to synchronize regions to be read of a sensor area of the camera with a position of the excitation light defined by the illumination control device, wherein the detection beam path includes an image splitter unit for splitting the emission light into multiple partial beam paths which each generate a partial image of the sample, the partial images lying next to one another such that linear regions in the partial images lie on the same line(s) of the sensor area.

Abstract: A light microscope has a light source for illuminating a specimen, a sensor array comprised of photon-counting detector elements for measuring detection light coming from the specimen, and a control device for controlling the sensor array. The control device is configured for flexibly binning the photon-counting detector elements into one or more super-pixels.

Abstract: An apparatus and method for light field microscopy. The apparatus has a light source for emitting excitation light, an excitation beam path for guiding the excitation light onto and into a sample, a two-dimensionally spatially resolving detector for detecting emission light emitted by the sample as a consequence of the irradiation by the excitation light, and a detection beam path having a microscope objective and a multi-lens array for guiding the emission light onto the two-dimensionally spatially resolving detector.

Abstract: A method for evaluating measurement data from a light field microscope, and an apparatus for light field microscopy wherein the following steps are carried out: a) at least one sample position to be analyzed is selected in a sample; b) images of the sample, which each contain a set of partial images, are recorded at a sequence of recording times using a light field microscope; c) the positions corresponding to the selected sample positions are determined in the partial images of the images recorded in step b); d) the image signals are extracted from at least some of the partial images at the positions determined in partial step c); e) an integrated signal is obtained for a certain recording time by virtue of the image signals extracted for a certain position from partial images of this recording time in step d) being integrated to form the integrated signal; and f) a time profile of the integrated signal is obtained by virtue of step e) being carried out for a plurality of recording times.

Abstract: A microscope and method of microscopy having a light source for providing illumination light, a controllable manipulation device for generating in a variable manner an illumination pattern of the illumination light to be selected, an illumination beam path with a microscope lens for guiding the illumination pattern to a sample to be examined, a detector having a plurality of pixels for examining the fluorescent light emitted by the sample, a detection beam path for guiding the fluorescent light emitted by the sample to the detector, a main beam splitter for splitting illumination light and fluorescent light, a control and evaluation unit for controlling the manipulation device and for evaluating the data measured by the detector. The manipulation device is arranged in the illumination beam path upstream from the main beam splitter such that the pixel of the detector can be individually activated using the control and evaluation unit and in read out patterns to be selected.

Abstract: The invention relates to a microscope having an excitation beam path for guiding excitation light, having a laser light source for providing a laser light beam as excitation light and having a scanning apparatus for aligning and moving a focused laser light beam in the entrance pupil of an illumination objective; wherein the laser focus is directed into an entrance point that is offset with respect to the optical axis of the illumination objective; and also having a detection beam path for guiding detection light, comprising a microlens array having a focal plane for generating partial imaged presentations and a detector arranged in the focal plane of the microlens array for capturing the partial imaged presentations. In addition, an evaluation unit for evaluating the captured image signals of the detector in accordance with light-field technology is present. The invention additionally relates to a method for microscopic image generation.

Abstract: A light microscope has a light source for illuminating a specimen, a photon-counting detector array with a plurality of photon-counting detector elements for measuring detection light coming from the specimen, wherein the photon-counting detector elements are configured to output respective measured photon count rates, and a control device for controlling the photon-counting detector array. The control device is configured to individually influence the measurable photon count rates which are simultaneously measurable with different photon-counting detector elements and/or which are consecutively measurable with the same photon-counting detector element. Furthermore, in an imaging method the measurable photon count rates of photon-counting detector elements are individually influenced to increase the signal-to-noise ratio for the photon-counting detector array.

Abstract: A method and apparatus for light field microscopy, wherein the following method steps are performed: a) measuring an image data record of a sample using a light field arrangement; b) creating at least one partial data record from the image data record; c) reconstructing a three-dimensional object from the partial data record created in step b).

Abstract: A device includes a detection path, along which detection radiation is guided, and a means for splitting the detection radiation between first and second detection paths. A detector has detector elements in each detection path. A microlens array is disposed upstream of each detector in a pupil. The first and second detectors have a substantially identical spatial resolution. The detector elements of the first detector are arranged line by line in a first line direction, while the detector elements of the second detector are arranged line by line in a second line direction. The first and second detectors are arranged relative to the image to be captured such that the first and second line directions are inclined relative to one another. A readout unit for reading out the image data of the detectors is configured for selectively reading those detector elements arranged line by line which form an image line.