Patents by Inventor Daniel Solis Escalante

Daniel Solis Escalante has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20220186265
    Abstract: Described herein is a method of producing a drimane sesquiterpene such as albicanol, drimenol and/or derivatives thereof by contacting at least one polypeptide with farnesyl diphosphate (FPP) with a polypeptide including a Haloacid dehalogenase (HAD)-like hydrolase domain and having bifunctional terpene synthase activity. The method may be performed in vitro or in vivo. Also described herein are amino acid sequences of polypeptides useful in the methods and nucleic acids encoding the polypeptides described. The described method further provides host cells or organisms genetically modified to express the polypeptides and useful to produce a drimane sesquiterpene such as albicanol, drimenol and/or derivatives thereof.
    Type: Application
    Filed: February 16, 2022
    Publication date: June 16, 2022
    Inventors: Michel Schalk, Pauline Anziani, Christian Goerner, Daniel Solis Escalante
  • Patent number: 11293037
    Abstract: Described herein is a method of producing a drimane sesquiterpene such as albicanol, drimenol and/or derivatives thereof by contacting at least one polypeptide with farnesyl diphosphate (FPP) with a polypeptide comprising a Haloacid dehalogenase (HAD)-like hydrolase domain and having bifunctional terpene synthase activity. The method may be performed in vitro or in vivo. Also described herein are amino acid sequences of polypeptides useful in the methods and nucleic acids encoding the polypeptides described. The described method further provides host cells or organisms genetically modified to express the polypeptides and useful to produce a drimane sesquiterpene such as albicanol, drimenol and/or derivatives thereof.
    Type: Grant
    Filed: May 31, 2018
    Date of Patent: April 5, 2022
    Assignee: FIRMENICH SA
    Inventors: Michel Schalk, Pauline Anziani, Christian Goerner, Daniel Solis Escalante
  • Patent number: 11214775
    Abstract: Provided herein are biocatalytic methods of producing terpene compounds by applying a novel type of phosphatase enzyme. The method allows the fully biochemical synthesis of terpene compounds, like for example copalol and labdendiol, and derivatives thereof, which serve as valuable intermediates for the production of perfumery ingredients, such as, for example, ambrox. Also provided are novel fully biochemical multistep processes for the production of such compounds as well as novel phosphatase enzymes and mutants and variants derived therefrom.
    Type: Grant
    Filed: July 10, 2019
    Date of Patent: January 4, 2022
    Inventors: Michel Schalk, Letizia Rocci, Daniel Solis Escalante
  • Publication number: 20210310031
    Abstract: Described herein are novel methods for the acetyl transferase-catalyzed production of drimanyl-acetate compounds by the acetylation of the respective drimanyl alcohol sources performed in vitro or in vivo. Also described herein is the identification of enzymes having corresponding acetyl transferase activity from different microbial and plant sources. Also described herein is the provision of enzyme mutants derived from the newly identified enzymes. Further described herein is the provision of corresponding coding sequences of such enzymes and mutants, recombinant vectors, and recombinant host cells suitable for the production of such acetyl transferases and mutants and for performing the novel production methods of drimanyl acetate compounds. Still further described herein is a method of using such drimanyl acetates as intermediates for the production of odorant, flavor or fragrance or insect/pest control ingredients.
    Type: Application
    Filed: October 14, 2019
    Publication date: October 7, 2021
    Inventors: Daniel SOLIS ESCALANTE, Christian GĂ–RNER, Laurent DAVIET, Qi WANG
  • Publication number: 20210222138
    Abstract: Provided herein are biocatalytic methods of producing terpene compounds by applying a novel type of phosphatase enzyme. The method allows the fully biochemical synthesis of terpene compounds, like for example copalol and labdendiol, and derivatives thereof, which serve as valuable intermediates for the production of perfumery ingredients, such as, for example, ambrox. Also provided are novel fully biochemical multistep processes for the production of such compounds as well as novel phosphatase enzymes and mutants and variants derived therefrom.
    Type: Application
    Filed: July 10, 2019
    Publication date: July 22, 2021
    Inventors: Michel Schalk, Letizia Rocci, Daniel Solis Escalante
  • Publication number: 20210010035
    Abstract: Described herein are methods of producing (+)-manool, the methods including: contacting geranylgeranyl diphosphate with a copalyl diphosphate (CPP) synthase to form a (9S, 10S)-copalyl diphosphate and contacting the CPP with a sclareol synthase enzyme to form (+)-manool and derivatives thereof. Also described herein are nucleic acids encoding CPP synthases and sclareol synthases for use in the methods. Further described herein are expression vectors and non-human host organisms and cells including nucleic acids encoding a CPP synthase and a sclareol synthase as described herein.
    Type: Application
    Filed: July 24, 2020
    Publication date: January 14, 2021
    Inventors: Michel Schalk, Laurent Daviet, Letizia Rocci, Daniel Solis Escalante
  • Patent number: 10752922
    Abstract: Described herein are methods of producing (+)-manool, the methods including: contacting geranylgeranyl diphosphate with a copalyl diphosphate (CPP) synthase to form a (9S, 10S)-copalyl diphosphate and contacting the CPP with a sclareol synthase enzyme to form (+)-manool and derivatives thereof. Also described herein are nucleic acids encoding CPP synthases and sclareol synthases for use in the methods. Further described herein are expression vectors and non-human host organisms and cells including nucleic acids encoding a CPP synthase and a sclareol synthase as described herein.
    Type: Grant
    Filed: December 18, 2017
    Date of Patent: August 25, 2020
    Assignee: Firmenich SA
    Inventors: Michel Schalk, Laurent Daviet, Letizia Rocci, Daniel Solis Escalante
  • Publication number: 20200140898
    Abstract: Described herein is a method of producing a drimane sesquiterpene such as albicanol, drimenol and/or derivatives thereof by contacting at least one polypeptide with farnesyl diphosphate (FPP) with a polypeptide comprising a Haloacid dehalogenase (HAD)-like hydrolase domain and having bifunctional terpene synthase activity. The method may be performed in vitro or in vivo. Also described herein are amino acid sequences of polypeptides useful in the methods and nucleic acids encoding the polypeptides described. The described method further provides host cells or organisms genetically modified to express the polypeptides and useful to produce a drimane sesquiterpene such as albicanol, drimenol and/or derivatives thereof.
    Type: Application
    Filed: May 31, 2018
    Publication date: May 7, 2020
    Inventors: Michel Schalk, Pauline Anziani, Christian Goerner, Daniel Solis Escalante
  • Publication number: 20190352673
    Abstract: Described herein are methods of producing (+)-manool, the methods including: contacting geranylgeranyl diphosphate with a copalyl diphosphate (CPP) synthase to form a (9S, 10S)-copalyl diphosphate and contacting the CPP with a sclareol synthase enzyme to form (+)-manool and derivatives thereof. Also described herein are nucleic acids encoding CPP synthases and sclareol synthases for use in the methods. Further described herein are expression vectors and non-human host organisms and cells including nucleic acids encoding a CPP synthase and a sclareol synthase as described herein.
    Type: Application
    Filed: December 18, 2017
    Publication date: November 21, 2019
    Inventors: Michel Schalk, Laurent Daviet, Letizia Rocci, Daniel Solis Escalante