Abstract: In accordance with an aspect of the present disclosure, a tissue imaging system includes a cassette configured to retain a tissue for imaging. The cassette includes a first cassette portion and a second cassette portion. The first cassette portion includes a recess formed in a central portion thereof, a window formed within the recess, and a first connection mechanism affixed thereto. The second cassette portion includes a cavity configured to compress a tissue therein and a second connection mechanism affixed thereto. An imaging device is configured to image the tissue through the window. Aligning the first connection mechanism and the second connection mechanism causes the first cassette portion to couple to the second cassette portion, compressing the tissue into the cavity for imaging.

Abstract: A system for tissue staining includes an upper housing, a lower housing, and a microscope lens. The upper housing includes a first reservoir configured to retain a solution therein, a valve attached to the reservoir including a turning knob, a first conduit configured to transport the solution, and a funnel fluidly connected to the first conduit. The lower housing is threadedly couped to the upper housing. The lower housing includes a top cassette portion having a window, a bottom cassette portion having a recess configured to retain a tissue therein, and a gasket disposed along an outer perimeter of the bottom cassette portion. The upper portion and the lower housing are coupled via threading. The microscope lens is configured to image the tissue through the window.

Abstract: A confocal microscope is provided that includes one or more lasers focused by an optical system into a line on the surface of a sample mounted to a stage. The microscope further includes, at least one linear array detector that is optically conjugated to the focused line. The stage permits movement of the sample with respect to all other components of the microscope, which remain stationary.

Abstract: An exhaust casing for a gas turbine engine comprises a shroud configured to surround an exhaust cone, and a heat shield attached to the shroud. The heat shield has a first end and a second end axially spaced apart from each other. A gap is defined radially between the shroud and the heat shield. The gap is configured to enclose at least a portion of an interface defined between at least one strut and the shroud. The exhaust casing is configured to surround the exhaust cone and configured to be connected to the exhaust cone via the at least one strut.

Abstract: The present invention provides compositions useful as inhibitors of SARM1 activity, pharmaceutical compositions thereof, and methods of using the same. The present invention provides dominant negative SARM1 molecules useful for treating a neurodegenerative or neurological disease or disorder, pharmaceutical compositions thereof, and methods of using the same.

Abstract: A gas turbine engine comprises a main gas path having an inner flow boundary wall and an outer flow boundary wall. A turbine exhaust case inner body defines a portion of the inner flow boundary wall of the main gas path. A lobed exhaust mixer defines a portion of the outer flow boundary wall of the main gas path. A stiffener ring is interconnected to at least a number lobes of the lobed exhaust mixer by a plurality of circumferentially spaced-apart struts extending through the main gas path. The stiffener ring is attached to the turbine exhaust case inner body by flexible features, such as circumferentially spaced-apart spring blades.

Abstract: A confocal microscope is provided that includes one or more lasers focused by an optical system into a line on the surface of a sample mounted to a stage. The microscope further includes, at least one linear array detector that is optically conjugated to the focused line. The stage permits movement of the sample with respect to all other components of the microscope, which remain stationary.

Abstract: A confocal microscope is provided that includes one or more lasers focused by an optical system into a line on the surface of a sample mounted to a stage. The microscope further includes at least one linear array detector that is optically conjugated to the focused line. The stage permits movement of the sample with respect to all other components of the microscope, which remain stationary.

Abstract: Methods for the differentiation of a 5-hydroxymethyl modification of a cytosine residue of interest in a nucleic acid molecule from (i) a 5-methyl modification of the cytosine residue of interest or (ii) an unmodified cytosine residue of interest, the methods making use of the capability of transcription-activator-like effector (TALE) proteins to preferentially bind with strong affinity to nucleic acid sequences containing non-modified cytosine residues or 5-methyl modified cytosine residues, and to bind, if at all, with only strongly reduced affinity to nucleic acid sequences containing 5-hydroxymethyl modified cytosine residues. The present invention further relates to respective uses of TALE proteins for the differentiation of a 5-hydroxymethyl modification of a cytosine residue of interest in a nucleic acid molecule from (i) a 5-methyl modification of the cytosine residue of interest or (ii) the unmodified cytosine residue of interest.

Abstract: A confocal microscope is provided that includes one or more lasers focused by an optical system into a line on the surface of a sample mounted to a stage. The microscope further includes at least one linear array detector that is optically conjugated to the focused line. The stage permits movement of the sample with respect to all other components of the microscope, which remain stationary.

Abstract: The present invention relates to methods for the differentiation of a 5-hydroxymethyl modification of a cytosine residue of interest in a nucleic acid molecule from (i) a 5-methyl modification of said cytosine residue of interest o,r (ii) said unmodified cytosine residue of interest, said methods making use of the capability of transcription-activator-like effector (TALE) proteins to preferentially bind with strong affinity to nucleic acid sequences containing non-modified cytosine residues or 5-methyl modified cytosine residues, and to bind, if at all, with only strongly reduced affinity to nucleic acid sequences containing 5-hydroxymethyl modified cytosine residues. The present invention further relates to respective uses of TALE proteins for the differentiation of a 5-hydroxymethyl modification of a cytosine residue of interest in a nucleic acid molecule from (i) a 5-methyl modification of said cytosine residue of interest or (ii) said unmodified cytosine residue of interest.

Abstract: The invention relates to orthogonal pairs of tRNAs and aminoacyl-tRNA synthetase that can incorporate unnatural amino acid into proteins produced in eubacterial host cells such as E. coli, or in a eukaryotic host such as a yeast cell. The invention provides, for example but not limited to, novel orthogonal synthetases, methods for identifying and making the novel synthetases, methods for producing proteins containing unnatural amino acids, and translation systems.

Abstract: The present invention relates to DNA polymerases with a special mutation which have an enhanced mismatch discrimination, the preparation and use thereof. The thermostable DNA polymerases with this mutation are particularly suitable for diagnostic and molecular-biological methods, e.g., allele-specific PCR.

Abstract: The invention relates to orthogonal pairs of tRNAs and aminoacyl-tRNA synthetase that can incorporate unnatural amino acid into proteins produced in eubacterial host cells such as E. coli, or in a eukaryotic host such as a yeast cell. The invention provides, for example but not limited to, novel orthogonal synthetases, methods for identifying and making the novel synthetases, methods for producing proteins containing unnatural amino acids, and translation systems.

Abstract: The invention relates to a method for isolation of target molecules from a nucleic acid population.

Abstract: The valve cover has a pair of tabs at the ends that are inserted into end sections of an orifice in the tray deck. The tabs of the valve cover abut against the underside of the tray deck while a pair of sloped portions of the valve cover rest on the top side of the tray deck in order to hold the valve cover in place.

Abstract: The invention relates to improved molecular-biological processing equipment and an improved method of processing biological samples. The invention combines the provision of biologically functional molecules such as nucleic acids and peptides and of derivatives or analogs of these two classes of molecules in miniaturized flow cells with the sequential addition of reagents or fluids and serves for the processing of biological samples, such as proteins, nucleic acids, biogenic small molecules such as e.g. metabolites, viruses or cells, which for this purpose are introduced into the miniaturized flow cells.

Abstract: The invention relates to an extraction method for isolating target molecules from a sample with the help of a molecule library.

Abstract: The valve cover has a pair of tabs at the ends that are inserted into end sections of an orifice in the tray deck. The tabs of the valve cover abut against the underside of the tray deck while a pair of sloped portions of the valve cover rest on the top side of the tray deck in order to hold the valve cover in place.

Abstract: The invention relates to orthogonal pairs of tRNAs and aminoacyl-tRNA synthetases that can incorporate unnatural amino acids into proteins produced in eubacterial host cells such as E. coli, or in a eukaryotic host such as a yeast cell. The invention provides, for example but not limited to, novel orthogonal synthetases, methods for identifying and making the novel synthetases, methods for producing proteins containing unnatural amino acids, and translation systems.