Abstract: The present invention relates to, inter alia, an engineered cell (e.g., iPSC, IPS-derived NK, or NK cell) comprising a disrupted B2M gene and an inserted polynucleotide encoding one or more of SERPINB9, a fusion of IL15 and IL15R?, and/or HLA-E. The engineered cell can further comprise a disrupted CIITA gene and an inserted polynucleotide encoding a CAR, wherein the CAR can be an anti-BCMA CAR or an anti-CD30 CAR. The engineered cell may further comprise a disrupted ADAM17 gene, a disrupted FAS gene, a disrupted CISH gene, and/or a disrupted REGNASE-1 gene. Methods for producing the engineered cells are also provided, and therapeutic uses of the engineered cells are also described. Guide RNA sequences targeting described target sequences are also described.

Abstract: There is provided a blower system for providing air to an airframe system, comprising a rotor configured to be mechanically coupled to a spool 440 of a gas turbine engine, wherein the rotor is configured to: in a blower mode, be driven to rotate by the spool to discharge air to an airframe discharge port for supply to an airframe system; and, in an engine drive mode, receive air from an external air source via an impingement port that is configured to direct the received air onto the rotor and thereby drive the rotor to rotate to drive the spool to.

Abstract: An auxiliary power unit (10) for an aircraft (1) comprises a gas turbine engine comprising an engine core (12). The engine core comprises a core compressor (14) and core turbine (18) coupled by a core shaft (24). The auxiliary power unit further comprises a load spool comprising a load compressor (30), a load turbine (20) and a permanent magnet electric machine (28), each being coupled by a load shaft (26). The load shaft (26) and core shaft (24) are configured to rotate independently of one another.

Abstract: There is provided a blower system for providing air to an airframe system, comprising a rotor configured to be mechanically coupled to a spool 440 of a gas turbine engine, wherein the rotor is configured to: in a blower mode, be driven to rotate by the spool to discharge air to an airframe discharge port for supply to an airframe system; and, in an engine drive mode, receive air from an external air source via an impingement port that is configured to direct the received air onto the rotor and thereby drive the rotor to rotate to drive the spool to.

Abstract: The present invention relates to, inter alia, an engineered cell (e.g., iPSC, IPS-derived NK, or NK cell) comprising a disrupted B2M gene and an inserted polynucleotide encoding one or more of SERPINB9, a fusion of IL15 and IL15R?, and/or HLA-E. The engineered cell can further comprise a disrupted CIITA gene and an inserted polynucleotide encoding a CAR, wherein the CAR can be an anti-BCMA CAR or an anti-CD30 CAR. The engineered cell may further comprise a disrupted ADAM17 gene, a disrupted FAS gene, a disrupted CISH gene, and/or a disrupted REGNASE-1 gene. Methods for producing the engineered cells are also provided, and therapeutic uses of the engineered cells are also described. Guide RNA sequences targeting described target sequences are also described.

Abstract: The present invention relates to, inter alia, an engineered cell (e.g., iPSC, IPS-derived NK, or NK cell) comprising a disrupted B2M gene and an inserted polynucleotide encoding one or more of SERPINB9, a fusion of IL15 and IL15R?, and/or HLA-E. The engineered cell can further comprise a disrupted CIITA gene and an inserted polynucleotide encoding a CAR, wherein the CAR can be an anti-BCMA CAR or an anti-CD30 CAR. The engineered cell may further comprise a disrupted ADAM17 gene, a disrupted FAS gene, a disrupted CISH gene, and/or a disrupted REGNASE-1 gene. Methods for producing the engineered cells are also provided, and therapeutic uses of the engineered cells are also described. Guide RNA sequences targeting described target sequences are also described.

Abstract: A gas turbine engine comprises, in fluid flow series, a gas-generator compressor, a combustor, a gas-generator turbine, and a free power turbine. The gas-generator compressor is an axi-centrifugal compressor comprising a plurality of axial compression stages followed by a single centrifugal compression stage, wherein the International Standard Atmosphere, sea-level static (hereinafter ISA SLS) design point pressure ratio of the axi-centrifugal compressor is from 12 to 16, and a ratio of the ISA SLS pressure rise across the axial compression stages to the ISA SLS pressure rise across the centrifugal compression stage is from 0.75 to 1.

Abstract: Provided herein are cells engineered to have improved protection against natural killer cell killing. The cells are engineered to comprise an insertion of a polynucleotide encoding SERPINB9. Also provided herein are methods of making the engineered cells and therapeutic uses of the engineered cells. The engineered cells can also comprise at least one genetic modification within or near at least one gene that encodes one or more MHC-I or MHC-II human leukocyte antigens or component or transcriptional regulator of the MHC-I or MHC-II complex, at least one genetic modification that increases the expression of at least one polynucleotide that encodes a tolerogenic factor, and optionally at least one genetic modification that increases or decreases the expression of at least one gene that encodes a survival factor. The engineered cells can be stem cells and the engineered stem cells can be differentiated into various lineages having protection against NK cell killing.

Abstract: Provided herein are cells engineered to have improved protection against natural killer cell killing. The cells are engineered to comprise an insertion of a polynucleotide encoding SERPINB9. Also provided herein are methods of making the engineered cells and therapeutic uses of the engineered cells. The engineered cells can also comprise at least one genetic modification within or near at least one gene that encodes one or more MHC-I or MHC-II human leukocyte antigens or component or transcriptional regulator of the MHC-I or MHC-II complex, at least one genetic modification that increases the expression of at least one polynucleotide that encodes a tolerogenic factor, and optionally at least one genetic modification that increases or decreases the expression of at least one gene that encodes a survival factor. The engineered cells can be stem cells and the engineered stem cells can be differentiated into various lineages having protection against NK cell killing.

Abstract: A gas turbine engine comprises, in fluid flow series, a gas-generator compressor, a combustor, a gas-generator turbine, and a free power turbine. The gas-generator compressor is an axi-centrifugal compressor comprising a plurality of axial compression stages followed by a single centrifugal compression stage, wherein the International Standard Atmosphere, sea-level static (hereinafter ISA SLS) design point pressure ratio of the axi-centrifugal compressor is from 12 to 16, and a ratio of the ISA SLS pressure rise across the axial compression stages to the ISA SLS pressure rise across the centrifugal compression stage is from 0.75 to 1.

Abstract: The present invention relates to, inter alia, an engineered cell (e.g., iPSC, IPS-derived NK, or NK cell) comprising a disrupted B2M gene and an inserted polynucleotide encoding one or more of SERPINB9, a fusion of IL15 and IL15R?, and/or HLA-E. The engineered cell can further comprise a disrupted CIITA gene and an inserted polynucleotide encoding a CAR, wherein the CAR can be an anti-BCMA CAR or an anti-CD30 CAR. The engineered cell may further comprise a disrupted ADAM17 gene, a disrupted FAS gene, a disrupted CISH gene, and/or a disrupted REGNASE-1 gene. Methods for producing the engineered cells are also provided, and therapeutic uses of the engineered cells are also described. Guide RNA sequences targeting described target sequences are also described.

Abstract: The present invention relates to, inter alia, an engineered cell (e.g., iPSC, IPS-derived NK, or NK cell) comprising a disrupted B2M gene and an inserted polynucleotide encoding one or more of SERPINB9, a fusion of IL15 and IL15R?, and/or HLA-E. The engineered cell can further comprise a disrupted CIITA gene and an inserted polynucleotide encoding a CAR, wherein the CAR can be an anti-BCMA CAR or an anti-CD30 CAR. The engineered cell may further comprise a disrupted ADAM17 gene, a disrupted FAS gene, a disrupted CISH gene, and/or a disrupted REGNASE-1 gene. Methods for producing the engineered cells are also provided, and therapeutic uses of the engineered cells are also described. Guide RNA sequences targeting described target sequences are also described.

Abstract: A gas turbine engine comprises, in fluid flow series, a gas-generator compressor, a combustor, a gas-generator turbine, and a free power turbine. The gas-generator compressor is an axi-centrifugal compressor comprising a plurality of axial compression stages followed by a single centrifugal compression stage, wherein the International Standard Atmosphere, sea-level static (hereinafter ISA SLS) design point pressure ratio of the axi-centrifugal compressor is from 12 to 16, and a ratio of the ISA SLS pressure rise across the axial compression stages to the ISA SLS pressure rise across the centrifugal compression stage is from 0.75 to 1.

Abstract: A turboshaft gas turbine engine comprises, in fluid flow series, a gas-generator compressor, a combustor, a gas-generator turbine, and a free power turbine. The gas-generator turbine is a single stage turbine having a specific work of from 200 to 350 kilojoules per kilogram, said specific work being the power output of the turbine per unit mass flow therethrough.

Abstract: A gas turbine engine comprises, in fluid flow series, a gas-generator compressor, a combustor, a gas-generator turbine, and a free power turbine. The gas-generator compressor is an axi-centrifugal compressor comprising a plurality of axial compression stages followed by a single centrifugal compression stage, wherein the International Standard Atmosphere, sea-level static (hereinafter ISA SLS) design point pressure ratio of the axi-centrifugal compressor is from 12 to 16, and a ratio of the ISA SLS pressure rise across the axial compression stages to the ISA SLS pressure rise across the centrifugal compression stage is from 0.75 to 1.

Abstract: An auxiliary power unit (10) for an aircraft (1) comprises a gas turbine engine comprising an engine core (12). The engine core comprises a core compressor (14) and core turbine (18) coupled by a core shaft (24). The auxiliary power unit further comprises a load spool comprising a load compressor (30), a load turbine (20) and a permanent magnet electric machine (28), each being coupled by a load shaft (26). The load shaft (26) and core shaft (24) are configured to rotate independently of one another.

Abstract: Narrowband quadrupole resonance (QR) probes were developed from thin-film high-temperature superconducting (HTS) resonators. The QR probes are useful in analyte-detection systems, in particular for the detection of nitrogen-containing compounds. Embodiments of the invention provide greater than an order of magnitude improvement in sensitivity and the ability to reject RF interference sources located outside the pass-band of a superconducting QR probe. Methods and apparatus are described for analyte detection and resonance frequency adjustment.

Abstract: Narrowband quadrupole resonance (QR) probes were developed from thin-film high-temperature superconducting (HTS) resonators. The QR probes are useful in analyte-detection systems, in particular for the detection of nitrogen-containing compounds. Embodiments of the invention provide greater than an order of magnitude improvement in sensitivity and the ability to reject RF interference sources located outside the pass-band of a superconducting QR probe. Methods and apparatus are described for analyte detection and resonance frequency adjustment.

Abstract: A vehicle mirror assembly having a housing or base member, a mirror lens and a bezel member. The mirror lens is provided in the shape of a portion of an end of an ellipsoid and has a varying radius of curvature. The center portion of the lens has a smaller radius of curvature than the outer portions along the major axis of the lens creating an improved field of view of the reflected image. The bezel is positioned over the intersection of the housing and mirror lens.

Abstract: A vehicle mirror assembly having a housing or base member, a mirror lens and a connecting gasket member. The mirror lens is provided in the shape of a portion of an end of an ellipsoid. The lens has a varying radius of curvature, The center portion of the lens has a smaller radius of curvature than the outer portions along the major axis of the lens creating an improved field of view of the reflected image.