Patents by Inventor Daniel Takashi Kamei

Daniel Takashi Kamei has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20220252598
    Abstract: In various embodiments devices and methods for the detection and/or quantification of clinically relevant pathogens (e.g., bacteria, fungi, viruses, etc.) are provided. In certain embodiments the device comprises a lateral-flow assay that detects the bacterium at a concentration of less than about 6×106 cells/mL, less than about 3×106 cells/ml, less than about 1×106 CFU/mL, or less than about 50 ?g/mL. In certain embodiments the device comprises an aqueous two-phase system (ATPS) comprising a mixed phase solution that separates into a first phase solution and a second phase solution; and a lateral-flow assay (LFA). In certain embodiments the device comprises a flow-through system comprising a concentration component comprising an aqueous two-phase system (ATPS) comprising a mixed phase solution that separates into a first phase solution and a second phase solution; and a detection component disposed beneath said concentration component.
    Type: Application
    Filed: February 16, 2022
    Publication date: August 11, 2022
    Applicant: The Regents of the University of California
    Inventors: Daniel Takashi Kamei, Yin To Chiu, Benjamin Ming Wu, Garrett L. Mosley
  • Publication number: 20220146507
    Abstract: Devices and methods are provided for the detection of CSF in a biological sample.
    Type: Application
    Filed: November 18, 2021
    Publication date: May 12, 2022
    Applicant: The Regents of the University of California
    Inventors: Daniel Takashi Kamei, Maie A. St. John, Zachary D. Taylor, Daniel William Bradbury, Ashley Elizabeth Kita
  • Patent number: 11327075
    Abstract: It was discovered that hydrogel scaffolds can be used to induce phase separation as aqueous two-phase systems (ATPSs) pass through and/or rehydrate the scaffolds, allowing for concentration of target analyte(s) (e.g., biomolecule(s)) into a particular phase of the ATPS or into a leading front. Accordingly, in various embodiments methods and devices are provided that utilize aqueous two-phase systems and hydrogel scaffolds to improve the sensitivity of assays (e.g., of point-of-care assays) without sacrificing cost or ease of use.
    Type: Grant
    Filed: August 21, 2017
    Date of Patent: May 10, 2022
    Assignee: The Regents of the University of California
    Inventors: Daniel Takashi Kamei, Benjamin Ming Wu, David Yuan Pereira, Chloe Michelle Wu, Matthew Foosing Yee, Joshua Anthony Keefe, Christina Caroline Pearce, Amir Adam Dailamy, Vincent K. Wong, Nguyen Khoi Le
  • Publication number: 20200284791
    Abstract: Disclosed herein are devices and methods that use aqueous two phase systems and lateral flow assays to detect target analytes in a sample. These devices and methods may be used to diagnose a disease or condition in a biological sample, such as blood or serum. In addition, these devices and methods may be used to detect allergens in a food samples or contaminants, such as environmental toxins, in water samples. Device and kit components may be conveniently assembled in a portable container and are amenable to actuation in most settings. The devices are simple to use, requiring a non-trained operator to simply add the sample to the device. Conveniently, the time it takes to detect the target analyte is very short. Thus, the devices and methods disclosed herein provide novel and useful means for point-of-care.
    Type: Application
    Filed: January 23, 2020
    Publication date: September 10, 2020
    Inventors: Daniel Takashi Kamei, Yin To Chiu, Benjamin Ming Wu, Garrett L. Mosley
  • Publication number: 20200150116
    Abstract: In various embodiments single-step ATPS paper-based diagnostic assays are provided that exploit the concept of sequential resolubilization of ATPS components to give rise to the desired phase separation behavior within paper. In one illustrative embodiment, a wick is provided for concentrating an analyte within an aqueous two-phase extraction system in a paper, where the wick comprises a paper configured to receive a sample where the paper comprises a first region containing a first component of an aqueous two-phase system (ATPS) where the first component is in a dry form, and a second region containing a second component of an aqueous two-phase system (ATPS) where the second component is in a dry form; and where said first region and the second region are disposed so that when said wick is contacted with a fluid sample, the first component of said ATPS is hydrated before the second component. In certain embodiments the first and second component are disposed so they are hydrated substantially simultaneously.
    Type: Application
    Filed: May 30, 2018
    Publication date: May 14, 2020
    Inventors: Daniel Takashi Kamei, Benjamin Ming Wu, Garrett L. Mosley, Yin To Chiu, David Yuan Pereira, Chloe Michelle Wu, Yue Han, So Youn Lee
  • Patent number: 10578616
    Abstract: Disclosed herein are devices and methods that use aqueous two phase systems and lateral flow assays to detect target analytes in a sample. These devices and methods may be used to diagnose a disease or condition in a biological sample, such as blood or serum. In addition, these devices and methods may be used to detect allergens in a food samples or contaminants, such as environmental toxins, in water samples. Device and kit components may be conveniently assembled in a portable container and are amenable to actuation in most settings. The devices are simple to use, requiring a non-trained operator to simply add the sample to the device. Conveniently, the time it takes to detect the target analyte is very short. Thus, the devices and methods disclosed herein provide novel and useful means for point-of-care.
    Type: Grant
    Filed: June 6, 2019
    Date of Patent: March 3, 2020
    Assignee: The Regents of the University of California
    Inventors: Daniel Takashi Kamei, Yin To Chiu, Benjamin Ming Wu, Garrett L. Mosley
  • Publication number: 20190391143
    Abstract: Disclosed herein are devices and methods that use aqueous two phase systems and lateral flow assays to detect target analytes in a sample. These devices and methods may be used to diagnose a disease or condition in a biological sample, such as blood or serum. In addition, these devices and methods may be used to detect allergens in a food samples or contaminants, such as environmental toxins, in water samples. Device and kit components may be conveniently assembled in a portable container and are amenable to actuation in most settings. The devices are simple to use, requiring a non-trained operator to simply add the sample to the device. Conveniently, the time it takes to detect the target analyte is very short. Thus, the devices and methods disclosed herein provide novel and useful means for point-of-care.
    Type: Application
    Filed: June 6, 2019
    Publication date: December 26, 2019
    Inventors: Daniel Takashi Kamei, Yin To Chiu, Benjamin Ming Wu, Garrett L. Mosley
  • Publication number: 20190250156
    Abstract: In various embodiments methods and devices are provided for the detection and/or quantification of an analyte. In certain embodiments a device is provided comprising an aqueous two-phase system (ATPS) comprising a mixed phase solution that separates into a first phase solution and a second phase where, in use, said first phase solution becomes a leading phase and said second phase solution becomes a lagging phase; a lateral-flow assay (LFA); and a probe and/or a development reagent, where in use, said probe associates with said first phase solution in said leading phase of said ATPS and/or said development reagent associates with said second phase solution in said lagging phase of said ATPS. In certain embodiments a “one-pot” system of purifying and amplifying a nucleic acid is provided utilizing, e.g., an ATPS and isothermal amplification reagents.
    Type: Application
    Filed: June 7, 2017
    Publication date: August 15, 2019
    Inventors: Daniel Takashi Kamei, Benjamin Ming Wu, Daniel William Bradbury, Shin Ting Sherine Frieda Cheung
  • Publication number: 20190187140
    Abstract: It was discovered that hydrogel scaffolds can be used to induce phase separation as aqueous two-phase systems (ATPSs) pass through and/or rehydrate the scaffolds, allowing for concentration of target analyte(s) (e.g., biomolecule(s)) into a particular phase of the ATPS or into a leading front. Accordingly, in various embodiments methods and devices are provided that utilize aqueous two-phase systems and hydrogel scaffolds to improve the sensitivity of assays (e.g., of point-of-care assays) without sacrificing cost or ease of use.
    Type: Application
    Filed: August 21, 2017
    Publication date: June 20, 2019
    Inventors: Daniel Takashi Kamei, Benjamin Ming Wu, David Yuan Pereira, Chloe Michelle Wu, Matthew Foosing Yee, Joshua Anthony Keefe, Christina Caroline Pearce, Amir Adam Dailamy, Vincent K. Wong, Nguyen Khoi Le