Patents by Inventor Daniel V. Lim
Daniel V. Lim has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 9116151Abstract: Disclosed is a method and associated device for the rapid identification of viable bacterial contaminants in food products. The method detects viable microbes by using a combined ATP-bioluminescence immunoassay. Escherichia coli O157:H7 and Salmonella enterica serovar Typhimurium were selected as target organisms in various matrices including ground beef homogenate, apple juice, milk, and phosphate-buffered saline. Specific antibodies were immobilized on the surface of well plates in which the sample matrices were incubated. The plates were washed, and the wells were incubated with BacTiter-Glo reagent in Mueller-Hinton II broth. Bioluminescent output was measured with a luminometer and signal-to-noise ratios were calculated. The LOD was not affected by the presence of non-target cells. A strong linear correlation was observed between the number of cells and luminescent output over 4 orders of magnitude.Type: GrantFiled: July 26, 2013Date of Patent: August 25, 2015Assignee: University of South FloridaInventors: Daniel V. Lim, Dawn M. Hunter
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Patent number: 8518658Abstract: Disclosed is a method and associated device for the rapid identification of viable bacterial contaminants in food products. The method detects viable microbes by using a combined ATP-bioluminescence immunoassay. Escherichia coli O157:H7 and Salmonella enterica serovar Typhimurium were selected as target organisms in various matrices including ground beef homogenate, apple juice, milk, and phosphate-buffered saline. Specific antibodies were immobilized on the surface of well plates in which the sample matrices were incubated. The plates were washed, and the wells were incubated with BacTiter-Glo reagent in Mueller-Hinton II broth. Bioluminescent output was measured with a luminometer and signal-to-noise ratios were calculated. The LOD was not affected by the presence of non-target cells. A strong linear correlation was observed between the number of cells and luminescent output over 4 orders of magnitude.Type: GrantFiled: April 27, 2010Date of Patent: August 27, 2013Assignee: University of South FloridaInventors: Daniel V. Lim, Dawn M. Hunter
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Publication number: 20110059462Abstract: An automated system for concentrating potentially harmful substances from various water types or other non-viscous liquids to facilitate detection of those substances is disclosed herein. The automated system comprises a water pressure driven or pump driven concentration unit that filters the test fluid through a hollow-fiber filter. Material collected on the filter is backflushed into a collection vessel by passing a small volume of sterile solution through the filter in the reverse direction. The automated system can be configured to be portable or to be integrated into a continuous liquid stream for online monitoring of test fluids. Optionally, an electronic signal at the end of the backflush sequence triggers a detector, such as an automated array biosensor, to begin processing and analyzing the sample.Type: ApplicationFiled: November 12, 2010Publication date: March 10, 2011Applicant: UNIVERSITY OF SOUTH FLORIDAInventors: Daniel V. Lim, Elizabeth A. Kearns, Stephaney D. Leskinen, Sonia MagaƱa
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Patent number: 7759639Abstract: An electrospray (ES)-based deposition system enabling the coating an impervious substrate, such as a glass slide, with biological materials in a vacuum. Distilled water or a buffer is used as the solvent; no other solvents are used thereby eliminating hazardous waste from the process. Movement across differential pumping stages causes evaporation of the solvent occurs resulting in shrinkage of the remaining constituents with an increase of the charge density. The resulting ion beam enters a vacuum chamber and the beam impinges on the substrate, whereby a thin layer is deposited thereon. The spray can be focused to a specific area allowing patterning of the substrate if desired. The amount of coating can be controlled and a specified number of coats of the same or different molecules can be added to the surface.Type: GrantFiled: August 20, 2007Date of Patent: July 20, 2010Assignee: University of South FloridaInventors: Rudiger Schlaf, Daniel V. Lim, Marianne F. Brannon, Anthony J. Cascio
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Publication number: 20080173595Abstract: An in-line water monitoring system for the detection of the accidental or intentional introduction of potentially harmful substances. The automated system comprises a water pressure driven concentration unit that filters drinking water through a hollow-fiber filter. Material collected on the filter is backflushed into a collection vessel by passing a sterile solution through the filter in the reverse direction. An electronic signal at the end of the backflush sequence triggers a sensor such as an array biosensor to begin processing and analyzing the sample. The array biosensor houses a slide prepared with antibodies to the test organism. The array biosensor is programmed to automatically run sample and detection reagents over the slide, analyze the resulting pattern for positive and negative data, and report the results.Type: ApplicationFiled: August 20, 2007Publication date: July 24, 2008Applicants: UNIVERSITY OF SOUTH FLORIDA, CONSTELLATION TECHNOLOGY CORPORATIONInventors: Daniel V. Lim, Elizabeth A. Kearns, Richard Darrell Sorrells, Timothy Arthur Postlewaite
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Publication number: 20080171152Abstract: An electrospray (ES)-based deposition system enabling the coating an impervious substrate, such as a glass slide, with biological materials in a vacuum. Distilled water or a buffer is used as the solvent; no other solvents are used thereby eliminating hazardous waste from the process. Movement across differential pumping stages causes evaporation of the solvent occurs resulting in shrinkage of the remaining constituents with an increase of the charge density. The resulting ion beam enters a vacuum chamber and the beam impinges on the substrate, whereby a thin layer is deposited thereon. The spray can be focused to a specific area allowing patterning of the substrate if desired. The amount of coating can be controlled and a specified number of coats of the same or different molecules can be added to the surface.Type: ApplicationFiled: August 20, 2007Publication date: July 17, 2008Applicant: UNIVERSITY OF SOUTH FLORIDAInventors: Rudiger Schlaf, Daniel V. Lim, Marianne F. Brannon, Anthony J. Cascio
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Patent number: 6476015Abstract: Substituted monocyclic &bgr;-lactam compounds that are N-1-thiolated monolactams, and which exhibit wide-ranging antibacterial activities, having a single 4-membered azetidinone ring in which: the N-1 nitrogen atom of the azetidinone ring is bonded to sulfur but is not sulfonated, of the formula: wherein R1-5 are independently hydrogen, alkyl, heteroalkyl, aryl, heteroaryl, alkenyl, or alkynyl; X is H, C, or O; and n=0-3. Chemical synthesis of these compounds by a [2+2]-imine-acid chloride cycloaddition, and methods for subsequent derivatization, are also disclosed. The compounds and compositions disclosed herein are useful as antibacterial and antibiotic agents.Type: GrantFiled: July 24, 2000Date of Patent: November 5, 2002Assignee: University of South FloridaInventors: Edward Turos, Edward T. Carpenter, Timothy Long, Daniel V. Lim, Sonja S. Dickey