Abstract: In some embodiments, an apparatus includes a calibration member, an imaging device and a proximity sensor. The calibration member is configured to be removably coupled to a deck of a liquid handling system. The calibration member has an alignment portion configured to matingly engage a portion of the deck such that a position of the calibration member is fixed with respect to the deck. The imaging device is coupled to the calibration member such that an axis of a lens of the imaging device intersects the deck at a first predetermined location relative to a deck reference point in at least a first dimension and a second dimension. The proximity sensor is coupled to the calibration member such that a calibration reference point on the proximity sensor is at a second predetermined location relative to the deck reference point in a third dimension.