Abstract: The present disclosure provides materials and methods for producing droplet libraries with thousands of distinct reagents. The monodispersed libraries prepared according to the methods provided herein use multiwall plates and are compatible with microfluidic processing.

Abstract: Testing devices are disclosed. In accordance with a first implementation, a testing device includes a sample chamber is to receive a sample and a reagent reservoir that contains a reagent used to determine a presence of a target molecule in the sample. A sample fluidic line is fluidically coupled to the sample chamber and a common fluidic line and a reagent fluidic line is fluidically coupled to the reagent reservoir and to the common fluidic line. A diagnostic indicator is coupled to the common fluidic line. The sample and the reagent flow through the respective sample fluidic line and the reagent fluidic line toward the common fluidic line and form a mixture within the common fluidic line and the common fluidic line enables a threshold incubation period of the mixture prior to the mixture flowing to the diagnostic indicator.

Abstract: The present disclosure provides materials and methods for detecting the presence of a target, such as a nucleic acid, biomolecule or biological particle, or virus genome in a sample. The present disclosure further provides point of care detection methods that cane done quickly and simply, optionally without sophisticated instrumentation. The present disclosure further provides improved methods for detecting and quantifying detection signals from various emulsions.