Abstract: Methods and systems are provided for sample preparation techniques and sequencing of macromolecular constituents of cells and other biological materials.

Abstract: The subject technology is related to methods and apparatus for discretization and manufacturability analysis of computer assisted design models. In one embodiment, the subject technology implements a computer-based method for the reception of an electronic file with a digital model representative of a physical object. The computer-based method determines geometric and physical attributes from a discretized version of the digital model, a cloud point version of the digital model, and symbolic functions generated through evolutionary algorithms. A set of predictive machine learning models is utilized to infer predictions related to the manufacture process of the physical object.

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization. Such polynucleotide processing may be useful for a variety of applications, including analyte characterization by polynucleotide sequencing. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, and RNA (e.g., mRNA or CRISPR guide RNAs). Also described herein, are barcoded labelling agents and oligonucleotide molecules useful for “tagging” analytes for characterization.

Abstract: A microfluidic emulsion droplet generation system and methods of use thereof are provided. The system may include a microfluidic substrate having a flow path configured and arranged for emulsion droplet generation, at least one textured surface in the flow path configured and arranged for inducing surface-mediated coalescence of emulsion droplets; and at least one channel junction in the flow path for emulsion droplet formation.

Abstract: Disclosed are devices, systems, kits, and methods for controlling liquid flow and, e.g., in particular, for forming droplet having substantially uniform droplet-to-droplet content. The devices, systems, and kits may include a first channel including a funnel or may include a first channel and a first-side channel, the first channel being in fluid communication with a droplet formation region. The devices, systems, and kits may further include a second channel fluidically connected to the first channel or the first side-channel. Funnels and/or side-channels may be used to enhance the control over particle spacing in the channels, thereby providing superior control over the number of particles of the same kind in formed droplets. The devices, systems, and kits of the invention may further include a mixer downstream of a channel intersection. Mixers can be used to reduce localized pockets of high concentration of dissolved ingredients.

Abstract: The present disclosure provides methods and systems for nucleic acid processing. A method for preparing a sequencing set may include providing a template nucleic acid and amplifying the template nucleic acid to provide a complementary nucleic acid. Next, the complementary nucleic acid may be fragmented and barcoded to produce a first set of barcoded fragments comprising a plurality of first barcoded fragments. Next, the plurality of first barcoded fragments may be fragmented to yield a second set of barcoded fragments comprising a plurality of second barcoded fragments.

Abstract: A microfluidic system includes a microfluidic chip having a plurality of fluid channels, each fluid channel having an opening providing access to an interior of the fluid channel, and a gasket disposable on the microfluidic chip in an aligned configuration, the gasket including a first side configured to face the microfluidic chip in the aligned configuration, a second side opposite the first side, and an aperture extending through the gasket from the first side to the second side, the aperture being sized and positioned to allow a communication of pressure from the second side of the gasket to the openings of at least two fluid channels when the gasket is in the aligned configuration.

Abstract: Devices, systems, and their methods of use, for generating and collecting droplets are provided. The device includes a collection region comprising a side wall canted at an angle. The invention further provides multiplex devices that increase droplet formation.

Abstract: Methods and systems are provided for sample preparation techniques and sequencing of macromolecular constituents of cells and other biological materials.

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and antigen screening. Polynucleotide processing may be useful for a variety of applications. Antigen screening may comprise the use of one or more engineered cells. Engineered cells may be useful for characterizing one or more analytes including, for example, a polypeptide antigen.

Abstract: The subject technology is related to methods and apparatus for training a set of regression machine learning models with a training set to produce a set of predictive values for a pending manufacturing request, the training set including data extracted from a set of manufacturing transactions submitted by a set of entities of a supply chain. A multi-objective optimization model is implemented to (1) receive an input including the set of predictive values and a set of features of a physical object, and (2) generate an output with a set of attributes associated with a manufacture of the physical object in response to receiving the input, the output complying with a multi-objective condition satisfied in the multi-objective optimization model.

Abstract: Microfluidic channels networks and systems are provided. One network includes a first fluid channel having a first depth dimension; at least a second channel intersecting the first channel at a first intersection; at least a third channel in fluid communication with the first intersection, at least one of the first intersection and the third channel having a depth dimension that is greater than the first depth dimension. Also provided is a flow control system for directing fluids in the network. Systems are additionally provided for flowing disrupted particles into a droplet formation junction, whereby a portion of the disrupted particles or the contents thereof are encapsulated into one or more droplets. Further provided is a method for controlling filling of a microfluidic network by controlling passive valving microfluidic channel network features.

Abstract: A stator angle is determined to correct a value measured by a wheel force transducer. A mounting bracket is rigidly attached to a vehicle and supports a housing within which a rotary encoder is mounted. A stator rod retainer is aligned with a rotational axis of the rotary encoder and has a through-bore extending perpendicular to the rotational axis. The stator rod retainer rotates relative to a stationary portion of the rotary encoder using at least one bearing, and the stator rod retainer supports a first end of a stator rod for substantially free movement through the through-bore. A controller determines, when the second end of the stator rod is fixedly attached to an encoder stator attached to a wheel, a stator angle of the stator rod used for adjusting at least one value associated with the wheel that is measured using the encoder stator.

Abstract: Microfluidic channels networks and systems are provided. One network includes a first fluid channel having a first depth dimension; at least a second channel intersecting the first channel at a first intersection; at least a third channel in fluid communication with the first intersection, at least one of the first intersection and the third channel having a depth dimension that is greater than the first depth dimension. Also provided is a flow control system for directing fluids in the network. Systems are additionally provided for flowing disrupted particles into a droplet formation junction, whereby a portion of the disrupted particles or the contents thereof are encapsulated into one or more droplets. Further provided is a method for controlling filling of a microfluidic network by controlling passive valving microfluidic channel network features.

Abstract: The subject technology is related to methods and apparatus for training a set of regression machine learning models with a training set to produce a set of predictive values for a pending manufacturing request, the training set including data extracted from a set of manufacturing transactions submitted by a set of entities of a supply chain. A multi-objective optimization model is implemented to (1) receive an input including the set of predictive values and a set of features of a physical object, and (2) generate an output with a set of attributes associated with a manufacture of the physical object in response to receiving the input, the output complying with a multi-objective condition satisfied in the multi-objective optimization model.

Abstract: Methods and systems are provided for sample preparation techniques and sequencing of macromolecular constituents of cells and other biological materials.

Abstract: An animal feeding system having a plurality of network connectable feeding stations, each with a base with a weight sensor for measuring a quantity of animal food placed in a food container, a processor, and network connection circuitry for connecting to a computer network. A server having a processor is coupled to a database to store pet information and use AI to analyze food and water consumption and recommend new foods or issue health alerts based on the consumption data. The server is configured for communication to feeding system bases and network connectable devices, such as smart phones, having executable food management software, wherein animal food intake information is transmitted to the server; and executable artificial intelligence loaded into and running on the server receives and processes data from the feeding stations and analyzes the data to make predictions and recommendations for foods individual animals prefer.

Abstract: A wheel force transducer stator angle correction apparatus. The apparatus includes a stator rod retainer having a through-bore, the stator rod retainer being in mechanical communication with at least one bearing. The apparatus also includes a stator rod having a first portion rigidly attached to an encoder stator attached to a wheel and having a second portion disposed within the through-bore of the stator rod retainer. The apparatus also includes an encoder rigidly attached to a portion of a vehicle associated with the wheel, wherein the encoder is adapted to measure an angle of the stator rod and to adjust at least one value associated with a wheel speed of the wheel based on the measured angle of the stator rod.

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization. Such polynucleotide processing may be useful for a variety of applications, including analyte characterization by polynucleotide sequencing. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, and RNA (e.g., mRNA or CRISPR guide RNAs). Also described herein, are barcoded labelling agents and oligonucleotide molecules useful for “tagging” analytes for characterization.

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization from a single cell. Such polynucleotide processing may be useful for a variety of applications. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins) and chromatin (e.g., accessible chromatin).