Patents by Inventor Daniela Castanotto

Daniela Castanotto has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • METHODS FOR INTRACELLULAR DELIVERY AND ENHANCED GENE TARGETING
    Publication number: 20230340480
    Abstract: Disclosed herein are methods and compositions for enhancing gene targeting. The method entails co-administrating to a cell a targeting molecule and a means of enhancing the function of the targeting molecule upon delivery to the cell. The means of enhancing the function of the targeting molecule including one or more of a stressor that induces cellular stress, a proton sponge molecule, and an endosome or lysosome inhibitor. Compositions disclosed include a targeting molecule and one or more of a stressor that induces cellular stress, a proton sponge molecule, and an endosome or lysosome inhibitor.
    Type: Application
    Filed: March 21, 2023
    Publication date: October 26, 2023
    Applicant: CITY OF HOPE
    Inventors: Daniela CASTANOTTO, Cy STEIN, Xiaowei ZHANG
  • TKI permeability enhancers
    Patent number: 11759530
    Abstract: Provided herein are, inter alia, methods useful for delivering nucleic acids and kinase inhibitors to a cell. The methods provided herein include the delivery of therapeutic nucleic acids to cancer cells by contacting a cancer cell with a kinase inhibitor and a therapeutic nucleic acid. The methods provided herein are therefore, inter alia, useful for the treatment of cancer.
    Type: Grant
    Filed: November 17, 2015
    Date of Patent: September 19, 2023
    Assignee: City of Hope
    Inventors: Cy Aaron Stein, Daniela Castanotto, David Horne
  • Methods for intracellular delivery and enhanced gene targeting
    Patent number: 11643655
    Abstract: Disclosed herein are methods and compositions for enhancing gene targeting. The method entails co-administrating to a cell a targeting molecule and a means of enhancing the function of the targeting molecule upon delivery to the cell. The means of enhancing the function of the targeting molecule including one or more of a stressor that induces cellular stress, a proton sponge molecule, and an endosome or lysosome inhibitor. Compositions disclosed include a targeting molecule and one or more of a stressor that induces cellular stress, a proton sponge molecule, and an endosome or lysosome inhibitor.
    Type: Grant
    Filed: November 15, 2017
    Date of Patent: May 9, 2023
    Assignee: CITY OF HOPE
    Inventors: Daniela Castanotto, Cy Stein, Xiaowei Zhang
  • METHODS FOR INTRACELLULAR DELIVERY AND ENHANCED GENE TARGETING
    Publication number: 20190330628
    Abstract: Disclosed herein are methods and compositions for enhancing gene targeting. The method entails co-administrating to a cell a targeting molecule and a means of enhancing the function of the targeting molecule upon delivery to the cell. The means of enhancing the function of the targeting molecule including one or more of a stressor that induces cellular stress, a proton sponge molecule, and an endosome or lysosome inhibitor. Compositions disclosed include a targeting molecule and one or more of a stressor that induces cellular stress, a proton sponge molecule, and an endosome or lysosome inhibitor.
    Type: Application
    Filed: November 15, 2017
    Publication date: October 31, 2019
    Inventors: Daniela CASTANOTTO, Cy STEIN, Xiaowei ZHANG
  • TKI PERMEABILITY ENHANCERS
    Publication number: 20190083650
    Abstract: Provided herein are, inter alia, methods useful for delivering nucleic acids and kinase inhibitors to a cell. The methods provided herein include the delivery of therapeutic nucleic acids to cancer cells by contacting a cancer cell with a kinase inhibitor and a therapeutic nucleic acid. The methods provided herein are therefore, inter alia, useful for the treatment of cancer.
    Type: Application
    Filed: November 17, 2015
    Publication date: March 21, 2019
    Inventors: Aaron Cy Stein, Daniela Castanotto, David Home
  • Methods and kits for synthesis of siRNA expression cassettes
    Patent number: 9133502
    Abstract: Amplification-based methods and kits for rapidly producing siRNA expression cassettes are provided. Also provided are methods for expressing amplified siRNA expression cassettes in cells.
    Type: Grant
    Filed: September 16, 2014
    Date of Patent: September 15, 2015
    Assignee: CITY OF HOPE
    Inventors: John J. Rossi, Daniela Castanotto
  • METHODS AND KITS FOR SYNTHESIS OF siRNA EXPRESSION CASSETTES
    Publication number: 20150072347
    Abstract: Amplification-based methods and kits for rapidly producing siRNA expression cassettes are provided. Also provided are methods for expressing amplified siRNA expression cassettes in cells.
    Type: Application
    Filed: September 16, 2014
    Publication date: March 12, 2015
    Applicant: CITY OF HOPE
    Inventors: John J. ROSSI, Daniela CASTANOTTO
  • Methods and kits for synthesis of siRNA expression cassettes
    Patent number: 8865676
    Abstract: Amplification-based methods and kits for rapidly producing siRNA expression cassettes are provided. Also provided are methods for expressing amplified siRNA expression cassettes in cells.
    Type: Grant
    Filed: January 31, 2013
    Date of Patent: October 21, 2014
    Assignee: City of Hope
    Inventors: John J. Rossi, Daniela Castanotto
  • METHODS AND KITS FOR SYNTHESIS OF siRNA EXPRESSION CASSETTES
    Publication number: 20130274141
    Abstract: Amplification-based methods and kits for rapidly producing siRNA expression cassettes are provided. Also provided are methods for expressing amplified siRNA expression cassettes in cells.
    Type: Application
    Filed: January 31, 2013
    Publication date: October 17, 2013
    Inventors: John J. ROSSI, Daniela CASTANOTTO
  • Double stranded nucleic acid targeting low copy promoter-specific RNA
    Patent number: 8513401
    Abstract: The present invention relates to transcriptional gene silencing (TGS) in mammalian, including human, cells that is mediated by small interfering RNA (siRNA) molecules. The present invention also relates to a double stranded nucleic acid that directs methylation of histones associated with target genes that produce low copy promoter-specific RNA. It has been found that siRNAs can be used to direct methylation of histones in mammalian, including human, cells.
    Type: Grant
    Filed: May 3, 2010
    Date of Patent: August 20, 2013
    Assignee: City of Hope
    Inventors: John J. Rossi, Daniela Castanotto, Gerd Pfeifer, Stella Tommasi, Kevin V. Morris, Daniel H. Kim
  • Methods and kits for synthesis of siRNA expression cassettes
    Patent number: 8389244
    Abstract: Amplification-based methods and kits for rapidly producing siRNA expression cassettes are provided. Also provided are methods for expressing amplified siRNA expression cassettes in cells.
    Type: Grant
    Filed: July 31, 2003
    Date of Patent: March 5, 2013
    Assignee: City of Hope
    Inventors: John J. Rossi, Daniela Castanotto
  • DOUBLE STRANDED NUCLEIC ACID TARGETING LOW COPY PROMOTER-SPECIFIC RNA
    Publication number: 20100267809
    Abstract: The present invention relates to transcriptional gene silencing (TGS) in mammalian, including human, cells that is mediated by small interfering RNA (siRNA) molecules. The present invention also relates to a double stranded nucleic acid that directs methylation of histones associated with target genes that produce low copy promoter-specific RNA. It has been found that siRNAs can be used to direct methylation of histones in mammalian, including human, cells.
    Type: Application
    Filed: May 3, 2010
    Publication date: October 21, 2010
    Applicant: CITY OF HOPE
    Inventors: John J. ROSSI, Daniela CASTANOTTO, Gerd PFEIFER, Stella TOMMASI, Kevin V. MORRIS, Daniel H. KIM
  • METHODS FOR DIRECTING DNA METHYLATION IN MAMMALIAN CELLS USING HOMOLOGOUS, SHORT DOUBLE STRANDED RNAS
    Publication number: 20070231907
    Abstract: The invention provides methods for methylating a gene of interest in a cell. The methods include exposing a mammalian cell to an siRNA molecule which is specific for a gene of interest in the cell. The methods also include introducing into the cell DNA sequences encoding a sense strand and an antisense strand of an siRNA which is specific for the gene of interest. The siRNA directs methylation of the gene of interest.
    Type: Application
    Filed: May 29, 2007
    Publication date: October 4, 2007
    Applicant: CITY OF HOPE
    Inventors: John ROSSI, Daniela CASTANOTTO, Gerd PFEIFFER, Stella TOMMASSI
  • Small interfering RNA mediated transcriptional gene silencing in mammalian cells
    Publication number: 20070104688
    Abstract: The present invention relates to transcriptional gene silencing (TGS) in mammalian, including human, cells that is mediated by small interfering RNA (siRNA) molecules. The present invention also relates to a method for directing histone and/or DNA methylation in mammalian, including human, cells. It has been found that siRNAs can be used to direct methylation of DNA in mammalian, including human, cells.
    Type: Application
    Filed: May 24, 2006
    Publication date: May 10, 2007
    Applicant: City of Hope
    Inventors: John Rossi, Daniela Castanotto, Gerd Pfeiffer, Stella Tommasi, Kevin Morris
  • Amplifying interfering RNA (RNAi) expression and effects
    Publication number: 20050227940
    Abstract: Methods for amplifying expression of interfering RNA (RNAi), and preferably siRNA or shRNA, using a RNAi (si/shRNA)-expressing concatamer, are disclosed. The methods are useful for modulating, including down-regulating and/or inhibiting, expression of a target gene in cells, including mammalian cells.
    Type: Application
    Filed: January 24, 2005
    Publication date: October 13, 2005
    Applicant: City of Hope
    Inventors: John Rossi, Daniela Castanotto, Laurence Cooper, Sergio Gonzalez
  • Methods for directing DNA methylation in mammalian cells using homologous short double stranded RNAs
    Publication number: 20040171118
    Abstract: The invention provides methods for methylating a gene of interest in a cell. The methods include exposing a mammalian cell to an siRNA molecule which is specific for a gene of interest in the cell. The methods also include introducing into the cell DNA sequences encoding a sense strand and an antisense strand of an siRNA which is specific for the gene of interest. The siRNA directs methylation of the gene of interest.
    Type: Application
    Filed: February 12, 2004
    Publication date: September 2, 2004
    Applicant: City of Hope
    Inventors: John J. Rossi, Daniela Castanotto, Gerd Pfeiffer, Stella Tommassi
  • Methods and kits for synthesis of siRNA expression cassettes
    Publication number: 20040091918
    Abstract: Amplification-based methods and kits for rapidly producing siRNA expression cassettes are provided. Also provided are methods for expressing amplified siRNA expression cassettes in cells.
    Type: Application
    Filed: July 31, 2003
    Publication date: May 13, 2004
    Applicant: City of Hope
    Inventors: John J. Rossi, Daniela Castanotto