Patents by Inventor Daniela Cucchetti
Daniela Cucchetti has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20230303962Abstract: An oleaginous yeast strain of the species Trichosporon oleaginosus, deposited on May 28, 2020 at Leibniz-Institut DSMZ, deposit number DSM 33530 can be used in the production of lipids by fermentation and was obtained by mutagenesis and subsequent selection from the oleaginous yeast strain of the species Trichosporon oleaginosus DSM 32508. The strain DSM 33530 is characterized by high thermotolerance, such as to allow the fermentation temperature to be increased compared to conventional processes and still guarantee the vital functions of the microorganism during fermentation itself.Type: ApplicationFiled: August 24, 2021Publication date: September 28, 2023Inventors: Daniele BIANCHI, Daniela CUCCHETTI, Concetta COMPAGNO, Silvia DONZELLA
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Patent number: 11162118Abstract: Process for the production of lipids from biomass including at least one polysaccharide comprising: —subjecting said biomass to hydrolysis to obtain a mixture comprising a first solid phase and a first aqueous phase; —preparing an inoculum comprising at least one oleaginous microorganism in a first fermentation device to obtain a first fermentation broth; —feeding said first aqueous phase and said first fermentation broth to a second fermentation device to obtain a second fermentation broth; —subjecting at least a portion of said second fermentation broth to microfiltration to obtain a first retentate and a first permeate; —feeding said first retentate to said second fermentation device; —subjecting said first permeate to a purification treatment to obtain a second permeate and a second retentate; —feeding said second retentate to said second fermentation device; —at the end of said fermentation, subjecting said second fermentation broth to separation to obtain an aqueous suspension of oleaginous cellular bioType: GrantFiled: April 22, 2015Date of Patent: November 2, 2021Assignee: Eni S.P.A.Inventors: Roberta Miglio, Daniela Cucchetti, Valentina Rodighiero
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Patent number: 11028415Abstract: A process for the production of lipids from biomass derived from guayule plants comprising: obtaining a hydrolysate comprising 5 carbon atom (C5) sugars from biomass derived from guayule plants, said 5 carbon atom (C5) sugars being present in said hydrolysate in a quantity greater than or equal to 80% by weight, preferably ranging from 85% by weight to 99% by weight, with respect to the total weight of said hydrolysate; feeding said hydrolysate to a fermentation device in the presence of at least one oleaginous yeast to obtain a fermentation broth; at the end of fermentation, subjecting said fermentation broth to separation obtaining an aqueous suspension of oleaginous cellular biomass comprising lipids and an aqueous phase. The lipids thus obtained can be advantageously used in the production of biofuel such as, for example, biodiesel or green diesel that can be used as such, or in mixtures with other fuels, for automotive transport.Type: GrantFiled: June 26, 2018Date of Patent: June 8, 2021Assignees: Versalis S.P.A., Eni S.P.A.Inventors: Daniela Cucchetti, Ezio Battistel, Chiara Gambaro, Alessandra D'Arminio Monforte
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Patent number: 10889841Abstract: The present invention concerns an oleaginous yeast variant of the species Rhodosporidium azoricum characterized by higher biomass yields and intra-cellular lipid accumulation useful for the production of bio-fuels higher, in determined conditions, with respect to the wild type strain of the same species. Furthermore, the invention concerns a method through which said oleaginous yeast variant of the species Rhodosporidium azoricum was obtained. The invention further concerns the lipid production by means of said variant strain of oleaginous yeast of the species Rhodosporidium azoricum.Type: GrantFiled: January 30, 2019Date of Patent: January 12, 2021Assignee: ENI S.p.A.Inventors: Giuliana Franzosi, Daniela Cucchetti, Daniele Bianchi, Silvia Galafassi, Concetta Compagno
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Publication number: 20200248214Abstract: A process for the production of lipids from biomass derived from guayule plants comprising: obtaining a hydrolysate comprising 5 carbon atom (C5) sugars from biomass derived from guayule plants, said 5 carbon atom (C5) sugars being present in said hydrolysate in a quantity greater than or equal to 80% by weight, preferably ranging from 85% by weight to 99% by weight, with respect to the total weight of said hydrolysate; feeding said hydrolysate to a fermentation device in the presence of at least one oleaginous yeast to obtain a fermentation broth; at the end of fermentation, subjecting said fermentation broth to separation obtaining an aqueous suspension of oleaginous cellular biomass comprising lipids and an aqueous phase. The lipids thus obtained can be advantageously used in the production of biofuel such as, for example, biodiesel or green diesel that can be used as such, or in mixtures with other fuels, for automotive transport.Type: ApplicationFiled: June 26, 2018Publication date: August 6, 2020Inventors: Daniela CUCCHETTI, Ezio BATTISTEL, Chiara GAMBARO, Alessandra D'ARMINIO MONFORTE
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Publication number: 20190161778Abstract: The present invention concerns an oleaginous yeast variant of the species Rhodosporidium azoricum characterized by higher biomass yields and intra-cellular lipid accumulation useful for the production of bio-fuels higher, in determined conditions, with respect to the wild type strain of the same species. Furthermore, the invention concerns a method through which said oleaginous yeast variant of the species Rhodosporidium azoricum was obtained. The invention further concerns the lipid production by means of said variant strain of oleaginous yeast of the species Rhodosporidium azoricum.Type: ApplicationFiled: January 30, 2019Publication date: May 30, 2019Applicant: ENI S.p.A.Inventors: Giuliana Franzosi, Daniela Cucchetti, Daniele Bianchi, Silvia Galafassi, Concetta Compagno
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Patent number: 10301656Abstract: The present invention concerns an oleaginous yeast variant of the species Rhodosporidium azoricum characterized by higher biomass yields and intra-cellular lipid accumulation useful for the production of bio-fuels higher, in determined conditions, with respect to the wild type strain of the same species. Furthermore, the invention concerns a method through which said oleaginous yeast variant of the species Rhodosporidium azoricum was obtained. The invention further concerns the lipid production by means of said variant strain of oleaginous yeast of the species Rhodosporidium azoricum.Type: GrantFiled: December 29, 2015Date of Patent: May 28, 2019Assignee: ENI S.p.A.Inventors: Giuliana Franzosi, Daniela Cucchetti, Daniele Bianchi, Silvia Galafassi, Concetta Compagno
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Patent number: 9885069Abstract: Process for the production of lipids from biomass including at least one polysaccharide, which comprises fermentation of a biomass hydrolysate using an oleaginous microorganism and separation of oleaginous cellular biomass comprising lipids. An aqueous fraction obtained by reverse osmosis of a spent fermentation broth is recycled as a feed for growth of the oleaginous microorganism. The lipids thus obtained can be advantageously used in the production of biodiesel or green diesel which can be used as such, or in a mixture with other automotive fuels.Type: GrantFiled: December 23, 2013Date of Patent: February 6, 2018Assignee: Eni S.p.A.Inventors: Rossella Bortolo, Roberta Miglio, Daniela Cucchetti
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Publication number: 20170369911Abstract: The present invention concerns an oleaginous yeast variant of the species Rhodosporidium azoricum characterized by higher biomass yields and intra- cellular lipid accumulation useful for the production of bio-fuels higher, in determined conditions, with respect to the wild type strain of the same species. Furthermore, the invention concerns a method through which said oleaginous yeast variant of the species Rhodosporidium azoricum was obtained. The invention further concerns the lipid production by means of said variant strain of oleaginous yeast of the species Rhodosporidium azoricum.Type: ApplicationFiled: December 29, 2015Publication date: December 28, 2017Applicant: ENI S.p.A.Inventors: Giuliana FRANZOSI, Daniela CUCCHETTI, Daniele BIANCHI, Silvia GALAFASSI, Concetta COMPAGNO
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Publication number: 20170088867Abstract: Process for the production of lipids from biomass including at least one polysaccharide comprising: —subjecting said biomass to hydrolysis to obtain a mixture comprising a first solid phase and a first aqueous phase; —preparing an inoculum comprising at least one oleaginous microorganism in a first fermentation device to obtain a first fermentation broth; —feeding said first aqueous phase and said first fermentation broth to a second fermentation device to obtain a second fermentation broth; —subjecting at least a portion of said second fermentation broth to microfiltration to obtain a first retentate and a first permeate; —feeding said first retentate to said second fermentation device; —subjecting said first permeate to a purification treatment to obtain a second permeate and a second retentate; —feeding said second retentate to said second fermentation device; —at the end of said fermentation, subjecting said second fermentation broth to separation to obtain an aqueous suspension of oleaginous cellular bioType: ApplicationFiled: April 22, 2015Publication date: March 30, 2017Inventors: Roberta Miglio, Daniela Cucchetti, Valentina Rodighiero
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Patent number: 9234221Abstract: Process for the production of lipids from biomass comprising polysaccharides. The biomass is subjected to acid hydrolysis to obtain a solid phase and an aqueous phase. The solid phase is subjected to acid or to enzymatic hydrolysis obtaining a further solid phase and a further aqueous phase. The aqueous phases obtained from both hydrolysis steps are subjected to fermentation with an oleaginous yeast. The obtained fermentation broth comprises oleaginous cellular biomass which is subjected to microfiltration for the isolation of lipids. Said lipids can be used in the production of biodiesel or green diesel.Type: GrantFiled: October 14, 2011Date of Patent: January 12, 2016Assignee: ENI S.P.A.Inventors: Giuliana Franzosi, Francesca Pizza, Daniela Cucchetti, Daniele Bianchi, Concetta Maria Compagno, Silvia Galafassi
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Publication number: 20150299746Abstract: Process for the production of lipids from biomass including at least one polysaccharide, which comprises fermentation of a biomass hydrolysate using an oleaginous microorganism and separation of oleaginous cellular biomass comprising lipids. An aqueous fraction obtained by reverse osmosis of a spent fermentation broth is recycled as a feed for growth of the oleaginous microorganism. The lipids thus obtained can be advantageously used in the production of biodiesel or green diesel which can be used as such, or in a mixture with other automotive fuels.Type: ApplicationFiled: December 23, 2013Publication date: October 22, 2015Applicant: ENI S.P.A.Inventors: Rossella Bortolo, Roberta Miglio, Daniela Cucchetti
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Publication number: 20130289289Abstract: Process for the production of lipids from biomass comprising polysaccharides. The biomass is subjected to acid hydrolysis to obtain a solid phase and an aqueous phase. The solid phase is subjected to acid or to enzymatic hydrolysis obtaining a further solid phase and a further aqueous phase. The aqueous phases obtained from both hydrolysis steps are subjected to fermentation with an oleaginous yeast. The obtained fermentation broth comprises oleaginous cellular biomass which is subjected to microfiltration for the isolation of lipids. Said lipids can be used in the production of biodiesel or green diesel.Type: ApplicationFiled: October 14, 2011Publication date: October 31, 2013Applicant: Eni S.P.A.Inventors: Giuliana Franzosi, Francesca Pizza, Daniela Cucchetti, Daniele Bianchi, Concetta Maria Compagno, Silvia Galafassi