Patents by Inventor Daniela Cucchetti

Daniela Cucchetti has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20230303962
    Abstract: An oleaginous yeast strain of the species Trichosporon oleaginosus, deposited on May 28, 2020 at Leibniz-Institut DSMZ, deposit number DSM 33530 can be used in the production of lipids by fermentation and was obtained by mutagenesis and subsequent selection from the oleaginous yeast strain of the species Trichosporon oleaginosus DSM 32508. The strain DSM 33530 is characterized by high thermotolerance, such as to allow the fermentation temperature to be increased compared to conventional processes and still guarantee the vital functions of the microorganism during fermentation itself.
    Type: Application
    Filed: August 24, 2021
    Publication date: September 28, 2023
    Inventors: Daniele BIANCHI, Daniela CUCCHETTI, Concetta COMPAGNO, Silvia DONZELLA
  • Patent number: 11162118
    Abstract: Process for the production of lipids from biomass including at least one polysaccharide comprising: —subjecting said biomass to hydrolysis to obtain a mixture comprising a first solid phase and a first aqueous phase; —preparing an inoculum comprising at least one oleaginous microorganism in a first fermentation device to obtain a first fermentation broth; —feeding said first aqueous phase and said first fermentation broth to a second fermentation device to obtain a second fermentation broth; —subjecting at least a portion of said second fermentation broth to microfiltration to obtain a first retentate and a first permeate; —feeding said first retentate to said second fermentation device; —subjecting said first permeate to a purification treatment to obtain a second permeate and a second retentate; —feeding said second retentate to said second fermentation device; —at the end of said fermentation, subjecting said second fermentation broth to separation to obtain an aqueous suspension of oleaginous cellular bio
    Type: Grant
    Filed: April 22, 2015
    Date of Patent: November 2, 2021
    Assignee: Eni S.P.A.
    Inventors: Roberta Miglio, Daniela Cucchetti, Valentina Rodighiero
  • Patent number: 11028415
    Abstract: A process for the production of lipids from biomass derived from guayule plants comprising: obtaining a hydrolysate comprising 5 carbon atom (C5) sugars from biomass derived from guayule plants, said 5 carbon atom (C5) sugars being present in said hydrolysate in a quantity greater than or equal to 80% by weight, preferably ranging from 85% by weight to 99% by weight, with respect to the total weight of said hydrolysate; feeding said hydrolysate to a fermentation device in the presence of at least one oleaginous yeast to obtain a fermentation broth; at the end of fermentation, subjecting said fermentation broth to separation obtaining an aqueous suspension of oleaginous cellular biomass comprising lipids and an aqueous phase. The lipids thus obtained can be advantageously used in the production of biofuel such as, for example, biodiesel or green diesel that can be used as such, or in mixtures with other fuels, for automotive transport.
    Type: Grant
    Filed: June 26, 2018
    Date of Patent: June 8, 2021
    Assignees: Versalis S.P.A., Eni S.P.A.
    Inventors: Daniela Cucchetti, Ezio Battistel, Chiara Gambaro, Alessandra D'Arminio Monforte
  • Patent number: 10889841
    Abstract: The present invention concerns an oleaginous yeast variant of the species Rhodosporidium azoricum characterized by higher biomass yields and intra-cellular lipid accumulation useful for the production of bio-fuels higher, in determined conditions, with respect to the wild type strain of the same species. Furthermore, the invention concerns a method through which said oleaginous yeast variant of the species Rhodosporidium azoricum was obtained. The invention further concerns the lipid production by means of said variant strain of oleaginous yeast of the species Rhodosporidium azoricum.
    Type: Grant
    Filed: January 30, 2019
    Date of Patent: January 12, 2021
    Assignee: ENI S.p.A.
    Inventors: Giuliana Franzosi, Daniela Cucchetti, Daniele Bianchi, Silvia Galafassi, Concetta Compagno
  • Publication number: 20200248214
    Abstract: A process for the production of lipids from biomass derived from guayule plants comprising: obtaining a hydrolysate comprising 5 carbon atom (C5) sugars from biomass derived from guayule plants, said 5 carbon atom (C5) sugars being present in said hydrolysate in a quantity greater than or equal to 80% by weight, preferably ranging from 85% by weight to 99% by weight, with respect to the total weight of said hydrolysate; feeding said hydrolysate to a fermentation device in the presence of at least one oleaginous yeast to obtain a fermentation broth; at the end of fermentation, subjecting said fermentation broth to separation obtaining an aqueous suspension of oleaginous cellular biomass comprising lipids and an aqueous phase. The lipids thus obtained can be advantageously used in the production of biofuel such as, for example, biodiesel or green diesel that can be used as such, or in mixtures with other fuels, for automotive transport.
    Type: Application
    Filed: June 26, 2018
    Publication date: August 6, 2020
    Inventors: Daniela CUCCHETTI, Ezio BATTISTEL, Chiara GAMBARO, Alessandra D'ARMINIO MONFORTE
  • Publication number: 20190161778
    Abstract: The present invention concerns an oleaginous yeast variant of the species Rhodosporidium azoricum characterized by higher biomass yields and intra-cellular lipid accumulation useful for the production of bio-fuels higher, in determined conditions, with respect to the wild type strain of the same species. Furthermore, the invention concerns a method through which said oleaginous yeast variant of the species Rhodosporidium azoricum was obtained. The invention further concerns the lipid production by means of said variant strain of oleaginous yeast of the species Rhodosporidium azoricum.
    Type: Application
    Filed: January 30, 2019
    Publication date: May 30, 2019
    Applicant: ENI S.p.A.
    Inventors: Giuliana Franzosi, Daniela Cucchetti, Daniele Bianchi, Silvia Galafassi, Concetta Compagno
  • Patent number: 10301656
    Abstract: The present invention concerns an oleaginous yeast variant of the species Rhodosporidium azoricum characterized by higher biomass yields and intra-cellular lipid accumulation useful for the production of bio-fuels higher, in determined conditions, with respect to the wild type strain of the same species. Furthermore, the invention concerns a method through which said oleaginous yeast variant of the species Rhodosporidium azoricum was obtained. The invention further concerns the lipid production by means of said variant strain of oleaginous yeast of the species Rhodosporidium azoricum.
    Type: Grant
    Filed: December 29, 2015
    Date of Patent: May 28, 2019
    Assignee: ENI S.p.A.
    Inventors: Giuliana Franzosi, Daniela Cucchetti, Daniele Bianchi, Silvia Galafassi, Concetta Compagno
  • Patent number: 9885069
    Abstract: Process for the production of lipids from biomass including at least one polysaccharide, which comprises fermentation of a biomass hydrolysate using an oleaginous microorganism and separation of oleaginous cellular biomass comprising lipids. An aqueous fraction obtained by reverse osmosis of a spent fermentation broth is recycled as a feed for growth of the oleaginous microorganism. The lipids thus obtained can be advantageously used in the production of biodiesel or green diesel which can be used as such, or in a mixture with other automotive fuels.
    Type: Grant
    Filed: December 23, 2013
    Date of Patent: February 6, 2018
    Assignee: Eni S.p.A.
    Inventors: Rossella Bortolo, Roberta Miglio, Daniela Cucchetti
  • Publication number: 20170369911
    Abstract: The present invention concerns an oleaginous yeast variant of the species Rhodosporidium azoricum characterized by higher biomass yields and intra- cellular lipid accumulation useful for the production of bio-fuels higher, in determined conditions, with respect to the wild type strain of the same species. Furthermore, the invention concerns a method through which said oleaginous yeast variant of the species Rhodosporidium azoricum was obtained. The invention further concerns the lipid production by means of said variant strain of oleaginous yeast of the species Rhodosporidium azoricum.
    Type: Application
    Filed: December 29, 2015
    Publication date: December 28, 2017
    Applicant: ENI S.p.A.
    Inventors: Giuliana FRANZOSI, Daniela CUCCHETTI, Daniele BIANCHI, Silvia GALAFASSI, Concetta COMPAGNO
  • Publication number: 20170088867
    Abstract: Process for the production of lipids from biomass including at least one polysaccharide comprising: —subjecting said biomass to hydrolysis to obtain a mixture comprising a first solid phase and a first aqueous phase; —preparing an inoculum comprising at least one oleaginous microorganism in a first fermentation device to obtain a first fermentation broth; —feeding said first aqueous phase and said first fermentation broth to a second fermentation device to obtain a second fermentation broth; —subjecting at least a portion of said second fermentation broth to microfiltration to obtain a first retentate and a first permeate; —feeding said first retentate to said second fermentation device; —subjecting said first permeate to a purification treatment to obtain a second permeate and a second retentate; —feeding said second retentate to said second fermentation device; —at the end of said fermentation, subjecting said second fermentation broth to separation to obtain an aqueous suspension of oleaginous cellular bio
    Type: Application
    Filed: April 22, 2015
    Publication date: March 30, 2017
    Inventors: Roberta Miglio, Daniela Cucchetti, Valentina Rodighiero
  • Patent number: 9234221
    Abstract: Process for the production of lipids from biomass comprising polysaccharides. The biomass is subjected to acid hydrolysis to obtain a solid phase and an aqueous phase. The solid phase is subjected to acid or to enzymatic hydrolysis obtaining a further solid phase and a further aqueous phase. The aqueous phases obtained from both hydrolysis steps are subjected to fermentation with an oleaginous yeast. The obtained fermentation broth comprises oleaginous cellular biomass which is subjected to microfiltration for the isolation of lipids. Said lipids can be used in the production of biodiesel or green diesel.
    Type: Grant
    Filed: October 14, 2011
    Date of Patent: January 12, 2016
    Assignee: ENI S.P.A.
    Inventors: Giuliana Franzosi, Francesca Pizza, Daniela Cucchetti, Daniele Bianchi, Concetta Maria Compagno, Silvia Galafassi
  • Publication number: 20150299746
    Abstract: Process for the production of lipids from biomass including at least one polysaccharide, which comprises fermentation of a biomass hydrolysate using an oleaginous microorganism and separation of oleaginous cellular biomass comprising lipids. An aqueous fraction obtained by reverse osmosis of a spent fermentation broth is recycled as a feed for growth of the oleaginous microorganism. The lipids thus obtained can be advantageously used in the production of biodiesel or green diesel which can be used as such, or in a mixture with other automotive fuels.
    Type: Application
    Filed: December 23, 2013
    Publication date: October 22, 2015
    Applicant: ENI S.P.A.
    Inventors: Rossella Bortolo, Roberta Miglio, Daniela Cucchetti
  • Publication number: 20130289289
    Abstract: Process for the production of lipids from biomass comprising polysaccharides. The biomass is subjected to acid hydrolysis to obtain a solid phase and an aqueous phase. The solid phase is subjected to acid or to enzymatic hydrolysis obtaining a further solid phase and a further aqueous phase. The aqueous phases obtained from both hydrolysis steps are subjected to fermentation with an oleaginous yeast. The obtained fermentation broth comprises oleaginous cellular biomass which is subjected to microfiltration for the isolation of lipids. Said lipids can be used in the production of biodiesel or green diesel.
    Type: Application
    Filed: October 14, 2011
    Publication date: October 31, 2013
    Applicant: Eni S.P.A.
    Inventors: Giuliana Franzosi, Francesca Pizza, Daniela Cucchetti, Daniele Bianchi, Concetta Maria Compagno, Silvia Galafassi