Patents by Inventor Daniela Munafo

Daniela Munafo has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Method for reducing adapter-dimer formation
    Patent number: 9650667
    Abstract: Methods are provided for ligating a 3? adapter and a 5? adapter to a target polynucleotide so as to avoid adapter dimer formation. Embodiments of the methods include adding a blocking oligonucleotide after the first ligation in which a 3? adapter is ligated to the target polynucleotide so that the blocking oligonucleotide is capable of hybridizing to excess 3? adapter and the ligated 3? adapter. Subsequently, a 5? adapter is ligated to the target polynucleotide thus avoiding adapter dimer formation.
    Type: Grant
    Filed: October 7, 2014
    Date of Patent: May 16, 2017
    Assignee: New England Biolabs, Inc.
    Inventors: Larry A. McReynolds, Daniela Munafo
  • Method for Reducing Adapter-Dimer Formation
    Publication number: 20150072870
    Abstract: Methods are provided for ligating a 3? adapter and a 5? adapter to a target polynucleotide so as to avoid adapter dimer formation. Embodiments of the methods include adding a blocking oligonucleotide after the first ligation in which a 3? adapter is ligated to the target polynucleotide so that the blocking oligonucleotide is capable of hybridizing to excess 3? adapter and the ligated 3? adapter. Subsequently, a 5? adapter is ligated to the target polynucleotide thus avoiding adapter dimer formation.
    Type: Application
    Filed: October 7, 2014
    Publication date: March 12, 2015
    Applicant: New England Biolabs, Inc.
    Inventors: Larry A. McReynolds, Daniela Munafo
  • Method for reducing adapter-dimer formation
    Patent number: 8883421
    Abstract: Methods are provided for ligating a 3? adapter and a 5? adapter to a target polynucleotide so as to avoid adapter dimer formation. Embodiments of the methods include adding a blocking oligonucleotide after the first ligation in which a 3? adapter is ligated to the target polynucleotide so that the blocking oligonucleotide is capable of hybridizing to excess 3? adapter and the ligated 3? adapter. Subsequently, a 5? adapter is ligated to the target polynucleotide thus avoiding adapter dimer formation.
    Type: Grant
    Filed: August 31, 2011
    Date of Patent: November 11, 2014
    Assignee: New England Biolabs, Inc.
    Inventors: Larry A. McReynolds, Daniela Munafo
  • Method for Reducing Adapter-Dimer Formation
    Publication number: 20130157869
    Abstract: Methods are provided for ligating a 3? adapter and a 5? adapter to a target polynucleotide so as to avoid adapter dimer formation. Embodiments of the methods include adding a blocking oligonucleotide after the first ligation in which a 3? adapter is ligated to the target polynucleotide so that the blocking oligonucleotide is capable of hybridizing to excess 3? adapter and the ligated 3? adapter. Subsequently, a 5? adapter is ligated to the target polynucleotide thus avoiding adapter dimer formation.
    Type: Application
    Filed: August 31, 2011
    Publication date: June 20, 2013
    Applicant: NEW ENGLAND BIOLABS, INC.
    Inventors: Larry A. McReynolds, Daniela Munafo