Patents by Inventor Daniele Malleo
Daniele Malleo has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 12138626Abstract: In situ-generated microfluidic isolation structures incorporating a solidified polymer network, methods of preparation and use, compositions and kits therefor are described. The ability to introduce in real time, a variety of isolating structures including pens and barriers offers improved methods of micro-object manipulation in microfluidic devices. The in situ-generated isolation structures may be permanently or temporarily installed.Type: GrantFiled: February 3, 2023Date of Patent: November 12, 2024Assignee: BRUKER CELLULAR ANALYSIS, INC.Inventors: Kristin G. Beaumont, Nan-Linda Ding, Volker L. S. Kurz, Troy A. Lionberger, Randall D. Lowe, Jr., Daniele Malleo, Andrew W. McFarland, J. Tanner Nevill, Xiaohua Wang
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Patent number: 12134758Abstract: Systems, methods and kits are described for culturing one or more biological cells in a microfluidic device, including provision of nutrients and gaseous components configured to enhance cell growth, viability, portability, or any combination thereof. In some embodiments, culturing a single cell may produce a clonal population in the microfluidic device.Type: GrantFiled: May 12, 2022Date of Patent: November 5, 2024Assignee: BRUKER CELLULAR ANALYSIS, INC.Inventors: Randall D. Lowe, Jr., Kristin G. Beaumont, Aathavan Karunakaran, Natalie C. Marks, Jason M. McEwen, Mark P. White, J. Tanner Nevill, Gang F. Wang, Andrew W. McFarland, Daniele Malleo, Keith J. Breinlinger, Xiao Guan, Kevin T. Chapman
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Publication number: 20240279588Abstract: Disclosed herein are cell processing systems, devices, and methods thereof. A system for planning parallel biomanufacturing processes comprises a plurality of cartridges, each containing a cell sample, a biomanufacturing workcell configured to receive the plurality of cartridges and comprising a plurality of instruments configured to interface with respective modules of each one of the plurality of cartridges to perform biomanufacturing processes on the cell sample therein, and a computing device comprising a processor configured to receive one or more inputs associated with the biomanufacturing processes to be performed on the cell sample within each of the plurality of cartridges, simulate the biomanufacturing processes based on the received one or more inputs, identify potential contentions based on the simulation, and generate an output based on the one or more inputs associated with the biomanufacturing processes.Type: ApplicationFiled: February 16, 2024Publication date: August 22, 2024Inventors: Daniele MALLEO, Marc ELPEL
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Publication number: 20240255537Abstract: Disclosed herein are cell processing and manufacturing systems, devices, and methods thereof. A system for cell processing may comprise a plurality of docking station modules each configured to perform, in cooperation with a corresponding plurality of cartridge modules, one or more cell processing operations upon cells within a cartridge.Type: ApplicationFiled: December 7, 2023Publication date: August 1, 2024Inventors: Daniele MALLEO, Wilson Wai TOY, Matthias WEBER
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Publication number: 20240254426Abstract: Described herein are systems, devices, and methods for high-throughput manufacturing of cell products for biomedical applications using automated systems executing parallel workflows. A method for scheduling a cell processing cartridge for loading into a cell processing system may include determining a system configuration, determining a configuration for each of a plurality of cell processing cartridges of the system, and providing a loading schedule for loading a first cartridge of the plurality of cartridges into the system. A method for monitoring contention of a cell processing system processing a plurality of cell samples in parallel may include loading the plurality of cell samples into the system and determining contention for each of a plurality of processing instruments before and after a processing operation is executed by the system. The contention may be based on a number of cell samples that need the instrument during a time period.Type: ApplicationFiled: January 12, 2024Publication date: August 1, 2024Inventors: Marc ELPEL, Stephen KULL, Daniele MALLEO, Howard SEAY
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Patent number: 11998914Abstract: Biological activity in holding pens in a micro-fluidic device can be assayed by placing in the holding pens capture objects that bind a particular material of interest produced by the biological activity. The biological material of interest that binds to each capture object can then be assessed, either in the micro-fluidic device or after exporting the capture object from the micro-fluidic device. The assessment can be utilized to characterize the biological activity in each holding pen. The biological activity can be production of the biological material of interest. Thus, the biological activity can correspond to or arise from one or more biological cells. Biological cells within a holding pen can be clonal cell colonies. The biological activity of each clonal cell colony can be assayed while maintaining the clonal status of each colony.Type: GrantFiled: March 24, 2022Date of Patent: June 4, 2024Assignee: BRUKER CELLULAR ANALYSIS, INC.Inventors: Kevin T. Chapman, Daniele Malleo, J. Tanner Nevill, Steven W. Short, Mark P. White, M. Jimena Loureiro
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Publication number: 20240165613Abstract: Disclosed herein are cell processing systems, devices, and methods thereof. A system for cell processing may comprise a plurality of instruments each independently configured to perform one or more cell processing operations upon a cartridge, and a robot capable of moving the cartridge between each of the plurality of instruments.Type: ApplicationFiled: October 16, 2023Publication date: May 23, 2024Inventors: Vladimir AZERSKY, Fabian GERLINGHAUS, Daniele MALLEO, Brian Alexander PESCH, Bharat S. THAKKAR, Wilson Wai TOY
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Publication number: 20230384573Abstract: A system for operating an electrokinetic device includes a support configured to hold and operatively couple with the electrokinetic device, an integrated electrical signal generation subsystem configured to apply a biasing voltage across a pair of electrodes in the electrokinetic device, and a light modulating subsystem configured to emit structured light onto the electrokinetic device. The system can further include a thermally controlled flow controller, and/or be configured to measure impedance across the electrokinetic device. The system can be a light microscope, including an optical train. The system can further include a light pipe, which can be part of the light modulating system, and which can be configured to supply light of substantially uniform intensity to the light modulating system or directly to the optical train.Type: ApplicationFiled: August 10, 2023Publication date: November 30, 2023Inventors: Andrew W. McFarland, Daniele Malleo, J. Tanner Nevill, Russell A. Newstrom, Keith J. Breinlinger, Paul M. Lundquist, Justin K. Valley, Jonathan Cloud Dragon Hubbard
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Patent number: 11826756Abstract: Disclosed herein are cell processing systems, devices, and methods thereof. A system for cell processing may comprise a plurality of instruments each independently configured to perform one or more cell processing operations upon a cartridge, and a robot capable of moving the cartridge between each of the plurality of instruments.Type: GrantFiled: April 28, 2023Date of Patent: November 28, 2023Assignee: Cellares CorporationInventors: Vladimir Azersky, Fabian Gerlinghaus, Daniele Malleo, Brian Alexander Pesch, Bharat S. Thakkar, Wilson Wai Toy
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MICROFLUIDIC DEVICES HAVING ISOLATION PENS AND METHODS OF TESTING BIOLOGICAL MICRO-OBJECTS WITH SAME
Publication number: 20230347347Abstract: A microfluidic device can comprise at least one swept region that is fluidically connected to unswept regions. The fluidic connections between the swept region and the unswept regions can enable diffusion but substantially no flow of media between the swept region and the unswept regions. The capability of biological micro-objects to produce an analyte of interest can be assayed in such a microfluidic device. Biological micro-objects in sample material loaded into a microfluidic device can be selected for particular characteristics and disposed into unswept regions. The sample material can then be flowed out of the swept region and an assay material flowed into the swept region. Flows of medium in the swept region do not substantially affect the biological micro-objects in the unswept regions, but any analyte of interest produced by a biological micro-object can diffuse from an unswept region into the swept region, where the analyte can react with the assay material to produce a localized detectable reaction.Type: ApplicationFiled: December 19, 2022Publication date: November 2, 2023Inventors: Mark P. White, Eric D. Hobbs, J. Tanner Nevill, Daniele Malleo, Steven W. Short -
Publication number: 20230321650Abstract: Disclosed herein are cell processing systems, devices, and methods thereof. A system for cell processing may comprise a plurality of instruments each independently configured to perform one or more cell processing operations upon a cartridge, and a robot capable of moving the cartridge between each of the plurality of instruments.Type: ApplicationFiled: April 28, 2023Publication date: October 12, 2023Inventors: Vladimir AZERSKY, Fabian GERLINGHAUS, Daniele MALLEO, Brian Alexander PESCH, Bharat S. THAKKAR, Wilson Wai TOY
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Patent number: 11701654Abstract: Disclosed herein are cell processing systems, devices, and methods thereof. A system for cell processing may comprise a plurality of instruments each independently configured to perform one or more cell processing operations upon a cartridge, and a robot capable of moving the cartridge between each of the plurality of instruments.Type: GrantFiled: November 22, 2022Date of Patent: July 18, 2023Assignee: Cellares CorporationInventors: Vladimir Azersky, Fabian Gerlinghaus, Daniele Malleo, Brian Alexander Pesch, Bharat S. Thakkar, Wilson Wai Toy
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Publication number: 20230182136Abstract: In situ-generated microfluidic isolation structures incorporating a solidified polymer network, methods of preparation and use, compositions and kits therefor are described. The ability to introduce in real time, a variety of isolating structures including pens and barriers offers improved methods of micro-object manipulation in microfluidic devices. The in situ-generated isolation structures may be permanently or temporarily installed.Type: ApplicationFiled: February 3, 2023Publication date: June 15, 2023Applicant: BERKELEY LIGHTS, INC.Inventors: Kristin G. Beaumont, Non-Linda Ding, Volker L.S. Kurz, Troy A. Lionberger, Randall D. Lowe, JR., Daniele Malleo, Andrew W. McFarland, J. Tanner Nevill, Xiaohua Wang
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Patent number: 11666913Abstract: In situ-generated microfluidic isolation structures incorporating a solidified polymer network, methods of preparation and use, compositions and kits therefor are described. The ability to introduce in real time, a variety of isolating structures including pens and barriers offers improved methods of micro-object manipulation in microfluidic devices. The in situ-generated isolation structures may be permanently or temporarily installed.Type: GrantFiled: November 22, 2016Date of Patent: June 6, 2023Assignee: BERKELEY LIGHTS, INCInventors: Kristin G. Beaumont, Nan-Linda Ding, Volker L. S. Kurz, Troy A. Lionberger, Randall D. Lowe, Jr., Daniele Malleo, Andrew W. McFarland, J. Tanner Nevill, Xiaohua Wang
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Microfluidic devices having isolation pens and methods of testing biological micro-objects with same
Patent number: 11565259Abstract: A microfluidic device can comprise at least one swept region that is fluidically connected to unswept regions. The fluidic connections between the swept region and the unswept regions can enable diffusion but substantially no flow of media between the swept region and the unswept regions. The capability of biological micro-objects to produce an analyte of interest can be assayed in such a microfluidic device. Biological micro-objects in sample material loaded into a microfluidic device can be selected for particular characteristics and disposed into unswept regions. The sample material can then be flowed out of the swept region and an assay material flowed into the swept region. Flows of medium in the swept region do not substantially affect the biological micro-objects in the unswept regions, but any analyte of interest produced by a biological micro-object can diffuse from an unswept region into the swept region, where the analyte can react with the assay material to produce a localized detectable reaction.Type: GrantFiled: November 14, 2019Date of Patent: January 31, 2023Assignee: Berkeley Lights, Inc.Inventors: Mark P. White, Eric D. Hobbs, J. Tanner Nevill, Daniele Malleo, Steven W. Short -
Publication number: 20220388003Abstract: Biological activity in holding pens in a micro-fluidic device can be assayed by placing in the holding pens capture objects that bind a particular material of interest produced by the biological activity. The biological material of interest that binds to each capture object can then be assessed, either in the micro-fluidic device or after exporting the capture object from the micro-fluidic device. The assessment can be utilized to characterize the biological activity in each holding pen. The biological activity can be production of the biological material of interest. Thus, the biological activity can correspond to or arise from one or more biological cells. Biological cells within a holding pen can be clonal cell colonies. The biological activity of each clonal cell colony can be assayed while maintaining the clonal status of each colony.Type: ApplicationFiled: March 24, 2022Publication date: December 8, 2022Inventors: Kevin T. Chapman, Daniele Malleo, J. Tanner Nevill, Steven W. Short, Mark P. White, M. Jimena Loureiro
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CAPTURING SPECIFIC NUCLEIC ACID MATERIALS FROM INDIVIDUAL BIOLOGICAL CELLS IN A MICRO-FLUIDIC DEVICE
Publication number: 20220379320Abstract: Individual biological cells can be selected in a micro-fluidic device and moved into isolation pens in the device. The cells can then be lysed in the pens, releasing nucleic acid material, which can be captured by one or more capture objects in the pens. The capture objects with the captured nucleic acid material can then be removed from the pens. The capture objects can include unique identifiers, allowing each capture object to be correlated to the individual cell from which the nucleic acid material captured by the object originated.Type: ApplicationFiled: March 15, 2022Publication date: December 1, 2022Inventors: Kevin T. Chapman, Eric D. Hobbs, Steven W. Short, Mark P. White, Daniele Malleo -
Publication number: 20220356429Abstract: Systems, methods and kits are described for culturing one or more biological cells in a microfluidic device, including provision of nutrients and gaseous components configured to enhance cell growth, viability, portability, or any combination thereof. In some embodiments, culturing a single cell may produce a clonal population in the microfluidic device.Type: ApplicationFiled: May 12, 2022Publication date: November 10, 2022Inventors: Randall D. LOWE, JR., Kristin G. BEAUMONT, Aathavan KARUNAKARAN, Natalie C. MARKS, Jason M. MCEWEN, Mark P. WHITE, J. Tanner NEVILL, Gang F. WANG, Andrew W. MCFARLAND, Daniele Malleo, Keith J. BREINLINGER, Xiao GUAN, Kevin T. CHAPMAN
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Patent number: 11365381Abstract: Systems, methods and kits are described for culturing one or more biological cells in a microfluidic device, including provision of nutrients and gaseous components configured to enhance cell growth, viability, portability, or any combination thereof. In some embodiments, culturing a single cell may produce a clonal population in the microfluidic device.Type: GrantFiled: July 2, 2020Date of Patent: June 21, 2022Assignee: Berkeley Lights, Inc.Inventors: Randall D. Lowe, Jr., Kristin G. Beaumont, Aathavan Karunakaran, Natalie C. Marks, Jason M. McEwen, Mark P. White, J. Tanner Nevill, Gang F. Wang, Andrew W. McFarland, Daniele Malleo, Keith J. Breinlinger, Xiao Guan, Kevin T. Chapman
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Publication number: 20220143612Abstract: A microfluidic apparatus is provided having one or more sequestration pens configured to isolate one or more target micro-objects by changing the orientation of the microfluidic apparatus with respect to a globally active force, such as gravity. Methods of selectively directing the movements of micro-objects in such a microfluidic apparatus using gravitational forces are also provided. The micro-objects can be biological micro-objects, such as cells, or inanimate micro-objects, such as beads.Type: ApplicationFiled: October 30, 2020Publication date: May 12, 2022Inventors: Keith J. Breinlinger, Eric D. Hobbs, Daniele Malleo, J. Tanner Nevill, Mark P. White