Patents by Inventor Daniele Malleo

Daniele Malleo has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 12134758
    Abstract: Systems, methods and kits are described for culturing one or more biological cells in a microfluidic device, including provision of nutrients and gaseous components configured to enhance cell growth, viability, portability, or any combination thereof. In some embodiments, culturing a single cell may produce a clonal population in the microfluidic device.
    Type: Grant
    Filed: May 12, 2022
    Date of Patent: November 5, 2024
    Assignee: BRUKER CELLULAR ANALYSIS, INC.
    Inventors: Randall D. Lowe, Jr., Kristin G. Beaumont, Aathavan Karunakaran, Natalie C. Marks, Jason M. McEwen, Mark P. White, J. Tanner Nevill, Gang F. Wang, Andrew W. McFarland, Daniele Malleo, Keith J. Breinlinger, Xiao Guan, Kevin T. Chapman
  • Publication number: 20240279588
    Abstract: Disclosed herein are cell processing systems, devices, and methods thereof. A system for planning parallel biomanufacturing processes comprises a plurality of cartridges, each containing a cell sample, a biomanufacturing workcell configured to receive the plurality of cartridges and comprising a plurality of instruments configured to interface with respective modules of each one of the plurality of cartridges to perform biomanufacturing processes on the cell sample therein, and a computing device comprising a processor configured to receive one or more inputs associated with the biomanufacturing processes to be performed on the cell sample within each of the plurality of cartridges, simulate the biomanufacturing processes based on the received one or more inputs, identify potential contentions based on the simulation, and generate an output based on the one or more inputs associated with the biomanufacturing processes.
    Type: Application
    Filed: February 16, 2024
    Publication date: August 22, 2024
    Inventors: Daniele MALLEO, Marc ELPEL
  • Publication number: 20240255537
    Abstract: Disclosed herein are cell processing and manufacturing systems, devices, and methods thereof. A system for cell processing may comprise a plurality of docking station modules each configured to perform, in cooperation with a corresponding plurality of cartridge modules, one or more cell processing operations upon cells within a cartridge.
    Type: Application
    Filed: December 7, 2023
    Publication date: August 1, 2024
    Inventors: Daniele MALLEO, Wilson Wai TOY, Matthias WEBER
  • Publication number: 20240254426
    Abstract: Described herein are systems, devices, and methods for high-throughput manufacturing of cell products for biomedical applications using automated systems executing parallel workflows. A method for scheduling a cell processing cartridge for loading into a cell processing system may include determining a system configuration, determining a configuration for each of a plurality of cell processing cartridges of the system, and providing a loading schedule for loading a first cartridge of the plurality of cartridges into the system. A method for monitoring contention of a cell processing system processing a plurality of cell samples in parallel may include loading the plurality of cell samples into the system and determining contention for each of a plurality of processing instruments before and after a processing operation is executed by the system. The contention may be based on a number of cell samples that need the instrument during a time period.
    Type: Application
    Filed: January 12, 2024
    Publication date: August 1, 2024
    Inventors: Marc ELPEL, Stephen KULL, Daniele MALLEO, Howard SEAY
  • Patent number: 11998914
    Abstract: Biological activity in holding pens in a micro-fluidic device can be assayed by placing in the holding pens capture objects that bind a particular material of interest produced by the biological activity. The biological material of interest that binds to each capture object can then be assessed, either in the micro-fluidic device or after exporting the capture object from the micro-fluidic device. The assessment can be utilized to characterize the biological activity in each holding pen. The biological activity can be production of the biological material of interest. Thus, the biological activity can correspond to or arise from one or more biological cells. Biological cells within a holding pen can be clonal cell colonies. The biological activity of each clonal cell colony can be assayed while maintaining the clonal status of each colony.
    Type: Grant
    Filed: March 24, 2022
    Date of Patent: June 4, 2024
    Assignee: BRUKER CELLULAR ANALYSIS, INC.
    Inventors: Kevin T. Chapman, Daniele Malleo, J. Tanner Nevill, Steven W. Short, Mark P. White, M. Jimena Loureiro
  • Publication number: 20240165613
    Abstract: Disclosed herein are cell processing systems, devices, and methods thereof. A system for cell processing may comprise a plurality of instruments each independently configured to perform one or more cell processing operations upon a cartridge, and a robot capable of moving the cartridge between each of the plurality of instruments.
    Type: Application
    Filed: October 16, 2023
    Publication date: May 23, 2024
    Inventors: Vladimir AZERSKY, Fabian GERLINGHAUS, Daniele MALLEO, Brian Alexander PESCH, Bharat S. THAKKAR, Wilson Wai TOY
  • Publication number: 20230384573
    Abstract: A system for operating an electrokinetic device includes a support configured to hold and operatively couple with the electrokinetic device, an integrated electrical signal generation subsystem configured to apply a biasing voltage across a pair of electrodes in the electrokinetic device, and a light modulating subsystem configured to emit structured light onto the electrokinetic device. The system can further include a thermally controlled flow controller, and/or be configured to measure impedance across the electrokinetic device. The system can be a light microscope, including an optical train. The system can further include a light pipe, which can be part of the light modulating system, and which can be configured to supply light of substantially uniform intensity to the light modulating system or directly to the optical train.
    Type: Application
    Filed: August 10, 2023
    Publication date: November 30, 2023
    Inventors: Andrew W. McFarland, Daniele Malleo, J. Tanner Nevill, Russell A. Newstrom, Keith J. Breinlinger, Paul M. Lundquist, Justin K. Valley, Jonathan Cloud Dragon Hubbard
  • Patent number: 11826756
    Abstract: Disclosed herein are cell processing systems, devices, and methods thereof. A system for cell processing may comprise a plurality of instruments each independently configured to perform one or more cell processing operations upon a cartridge, and a robot capable of moving the cartridge between each of the plurality of instruments.
    Type: Grant
    Filed: April 28, 2023
    Date of Patent: November 28, 2023
    Assignee: Cellares Corporation
    Inventors: Vladimir Azersky, Fabian Gerlinghaus, Daniele Malleo, Brian Alexander Pesch, Bharat S. Thakkar, Wilson Wai Toy
  • Publication number: 20230347347
    Abstract: A microfluidic device can comprise at least one swept region that is fluidically connected to unswept regions. The fluidic connections between the swept region and the unswept regions can enable diffusion but substantially no flow of media between the swept region and the unswept regions. The capability of biological micro-objects to produce an analyte of interest can be assayed in such a microfluidic device. Biological micro-objects in sample material loaded into a microfluidic device can be selected for particular characteristics and disposed into unswept regions. The sample material can then be flowed out of the swept region and an assay material flowed into the swept region. Flows of medium in the swept region do not substantially affect the biological micro-objects in the unswept regions, but any analyte of interest produced by a biological micro-object can diffuse from an unswept region into the swept region, where the analyte can react with the assay material to produce a localized detectable reaction.
    Type: Application
    Filed: December 19, 2022
    Publication date: November 2, 2023
    Inventors: Mark P. White, Eric D. Hobbs, J. Tanner Nevill, Daniele Malleo, Steven W. Short
  • Publication number: 20230321650
    Abstract: Disclosed herein are cell processing systems, devices, and methods thereof. A system for cell processing may comprise a plurality of instruments each independently configured to perform one or more cell processing operations upon a cartridge, and a robot capable of moving the cartridge between each of the plurality of instruments.
    Type: Application
    Filed: April 28, 2023
    Publication date: October 12, 2023
    Inventors: Vladimir AZERSKY, Fabian GERLINGHAUS, Daniele MALLEO, Brian Alexander PESCH, Bharat S. THAKKAR, Wilson Wai TOY
  • Patent number: 11701654
    Abstract: Disclosed herein are cell processing systems, devices, and methods thereof. A system for cell processing may comprise a plurality of instruments each independently configured to perform one or more cell processing operations upon a cartridge, and a robot capable of moving the cartridge between each of the plurality of instruments.
    Type: Grant
    Filed: November 22, 2022
    Date of Patent: July 18, 2023
    Assignee: Cellares Corporation
    Inventors: Vladimir Azersky, Fabian Gerlinghaus, Daniele Malleo, Brian Alexander Pesch, Bharat S. Thakkar, Wilson Wai Toy
  • Publication number: 20230182136
    Abstract: In situ-generated microfluidic isolation structures incorporating a solidified polymer network, methods of preparation and use, compositions and kits therefor are described. The ability to introduce in real time, a variety of isolating structures including pens and barriers offers improved methods of micro-object manipulation in microfluidic devices. The in situ-generated isolation structures may be permanently or temporarily installed.
    Type: Application
    Filed: February 3, 2023
    Publication date: June 15, 2023
    Applicant: BERKELEY LIGHTS, INC.
    Inventors: Kristin G. Beaumont, Non-Linda Ding, Volker L.S. Kurz, Troy A. Lionberger, Randall D. Lowe, JR., Daniele Malleo, Andrew W. McFarland, J. Tanner Nevill, Xiaohua Wang
  • Patent number: 11666913
    Abstract: In situ-generated microfluidic isolation structures incorporating a solidified polymer network, methods of preparation and use, compositions and kits therefor are described. The ability to introduce in real time, a variety of isolating structures including pens and barriers offers improved methods of micro-object manipulation in microfluidic devices. The in situ-generated isolation structures may be permanently or temporarily installed.
    Type: Grant
    Filed: November 22, 2016
    Date of Patent: June 6, 2023
    Assignee: BERKELEY LIGHTS, INC
    Inventors: Kristin G. Beaumont, Nan-Linda Ding, Volker L. S. Kurz, Troy A. Lionberger, Randall D. Lowe, Jr., Daniele Malleo, Andrew W. McFarland, J. Tanner Nevill, Xiaohua Wang
  • Patent number: 11565259
    Abstract: A microfluidic device can comprise at least one swept region that is fluidically connected to unswept regions. The fluidic connections between the swept region and the unswept regions can enable diffusion but substantially no flow of media between the swept region and the unswept regions. The capability of biological micro-objects to produce an analyte of interest can be assayed in such a microfluidic device. Biological micro-objects in sample material loaded into a microfluidic device can be selected for particular characteristics and disposed into unswept regions. The sample material can then be flowed out of the swept region and an assay material flowed into the swept region. Flows of medium in the swept region do not substantially affect the biological micro-objects in the unswept regions, but any analyte of interest produced by a biological micro-object can diffuse from an unswept region into the swept region, where the analyte can react with the assay material to produce a localized detectable reaction.
    Type: Grant
    Filed: November 14, 2019
    Date of Patent: January 31, 2023
    Assignee: Berkeley Lights, Inc.
    Inventors: Mark P. White, Eric D. Hobbs, J. Tanner Nevill, Daniele Malleo, Steven W. Short
  • Publication number: 20220388003
    Abstract: Biological activity in holding pens in a micro-fluidic device can be assayed by placing in the holding pens capture objects that bind a particular material of interest produced by the biological activity. The biological material of interest that binds to each capture object can then be assessed, either in the micro-fluidic device or after exporting the capture object from the micro-fluidic device. The assessment can be utilized to characterize the biological activity in each holding pen. The biological activity can be production of the biological material of interest. Thus, the biological activity can correspond to or arise from one or more biological cells. Biological cells within a holding pen can be clonal cell colonies. The biological activity of each clonal cell colony can be assayed while maintaining the clonal status of each colony.
    Type: Application
    Filed: March 24, 2022
    Publication date: December 8, 2022
    Inventors: Kevin T. Chapman, Daniele Malleo, J. Tanner Nevill, Steven W. Short, Mark P. White, M. Jimena Loureiro
  • Publication number: 20220379320
    Abstract: Individual biological cells can be selected in a micro-fluidic device and moved into isolation pens in the device. The cells can then be lysed in the pens, releasing nucleic acid material, which can be captured by one or more capture objects in the pens. The capture objects with the captured nucleic acid material can then be removed from the pens. The capture objects can include unique identifiers, allowing each capture object to be correlated to the individual cell from which the nucleic acid material captured by the object originated.
    Type: Application
    Filed: March 15, 2022
    Publication date: December 1, 2022
    Inventors: Kevin T. Chapman, Eric D. Hobbs, Steven W. Short, Mark P. White, Daniele Malleo
  • Publication number: 20220356429
    Abstract: Systems, methods and kits are described for culturing one or more biological cells in a microfluidic device, including provision of nutrients and gaseous components configured to enhance cell growth, viability, portability, or any combination thereof. In some embodiments, culturing a single cell may produce a clonal population in the microfluidic device.
    Type: Application
    Filed: May 12, 2022
    Publication date: November 10, 2022
    Inventors: Randall D. LOWE, JR., Kristin G. BEAUMONT, Aathavan KARUNAKARAN, Natalie C. MARKS, Jason M. MCEWEN, Mark P. WHITE, J. Tanner NEVILL, Gang F. WANG, Andrew W. MCFARLAND, Daniele Malleo, Keith J. BREINLINGER, Xiao GUAN, Kevin T. CHAPMAN
  • Patent number: 11365381
    Abstract: Systems, methods and kits are described for culturing one or more biological cells in a microfluidic device, including provision of nutrients and gaseous components configured to enhance cell growth, viability, portability, or any combination thereof. In some embodiments, culturing a single cell may produce a clonal population in the microfluidic device.
    Type: Grant
    Filed: July 2, 2020
    Date of Patent: June 21, 2022
    Assignee: Berkeley Lights, Inc.
    Inventors: Randall D. Lowe, Jr., Kristin G. Beaumont, Aathavan Karunakaran, Natalie C. Marks, Jason M. McEwen, Mark P. White, J. Tanner Nevill, Gang F. Wang, Andrew W. McFarland, Daniele Malleo, Keith J. Breinlinger, Xiao Guan, Kevin T. Chapman
  • Publication number: 20220143612
    Abstract: A microfluidic apparatus is provided having one or more sequestration pens configured to isolate one or more target micro-objects by changing the orientation of the microfluidic apparatus with respect to a globally active force, such as gravity. Methods of selectively directing the movements of micro-objects in such a microfluidic apparatus using gravitational forces are also provided. The micro-objects can be biological micro-objects, such as cells, or inanimate micro-objects, such as beads.
    Type: Application
    Filed: October 30, 2020
    Publication date: May 12, 2022
    Inventors: Keith J. Breinlinger, Eric D. Hobbs, Daniele Malleo, J. Tanner Nevill, Mark P. White
  • Patent number: 11318479
    Abstract: Individual biological cells can be selected in a micro-fluidic device and moved into isolation pens in the device. The cells can then be lysed in the pens, releasing nucleic acid material, which can be captured by one or more capture objects in the pens. The capture objects with the captured nucleic acid material can then be removed from the pens. The capture objects can include unique identifiers, allowing each capture object to be correlated to the individual cell from which the nucleic acid material captured by the object originated.
    Type: Grant
    Filed: December 18, 2014
    Date of Patent: May 3, 2022
    Assignee: Berkeley Lights, Inc.
    Inventors: Kevin T. Chapman, Eric D. Hobbs, Steven W. Short, Mark P. White, Daniele Malleo