Abstract: A cocktail dispensing assembly includes a housing that is positionable in an establishment licensed to sell alcohol and a dispensing outlet is integrated into the housing. An alcohol dispensing unit is integrated into the housing to dispense a pre-determined volume of a distilled spirit outwardly through the dispensing outlet. A mixer dispensing unit is integrated into the housing to dispense a pre-determined volume of a cocktail mixer outwardly through the dispensing outlet. In this way the distilled spirit and the mixer can be dispensed in the beverage container. A point of sale unit is integrated into the housing to conduct a financial transaction to purchase a cocktail. An identification reader is integrated into the housing to read a state issued identification card. A facial recognition unit is integrated into the housing to scan a face of a user for to inhibiting an unauthorized user from purchasing alcohol.

Abstract: Protected triazabutadiene molecules, such as those according to Formula D or variations thereof, as probes for cellular studies in intact biological systems. The protected triazabutadiene probes selectively release benzene diazonium ions (BDIs) intracellularly, providing a tool for accessing and/or labeling intracellular proteins or molecules prior to cell lysis. The present invention also includes methods for synthesizing the protected triazabutadienes, the protected triazabutadienes themselves, and methods of use.

Abstract: Methods are disclosed herein for quantitative multiplex PCR. The methods proceed through at least two successive rounds of amplification, for example, a target enrichment step and a target amplification step.

Abstract: Disclosed are methods for quantitative multiplex PCR in which any combination of target sequences can be paired with each other. The reaction mix for use in these methods can be assembled all at one time and the method performed without additional manipulation between the first and last rounds of replication.

Abstract: Methods and systems for real-time, interactive solutioning and visualization of working capital for an enterprise employ a processor coupled to memory and other computer hardware and software components for storing fixed data consisting at least in part of information regarding financial institution products and information regarding governmental regulations; storing variable data consisting at least in part of information regarding operations of the enterprise; receiving variable data interactively consisting at least in part of information regarding enterprise preferences; and generating at least one recommendation for a proposed structure for the enterprise based at least in part on the fixed data and the variable data.