Abstract: Recombinant or synthetic polynucleotide sequences comprising three sucking pest resistant genes including an insecticidal Tma12 gene (SEQ ID NO: 18), a PTA gene (SEQ ID NO: 19) and ASAL gene (SEQ ID NO: 20) and a herbicidal Re-PAT gene (SEQ ID NO: 17), transformed in a mono/dicot plant, that are encoded to provide insecticidal and herbicidal toxin proteins in a transgenic plant with constitutively targeted expression, resulting in the decreased resistance development against insecticidal toxins proteins and increased efficacy against the insect mortality, particularly whitefly and jassid. A method or an assay for detecting the presence of transgenic event Kalgin-5 based on the DNA sequence of the recombinant polynucleotide construct inserted into the genome of the transgenic plant and the genomic sequences flanking the insertion site.

Abstract: Recombinant or synthetic polynucleotide sequences comprising one Re-PAT gene identified as SEQ ID NO: 11 and two B. thuringiensis ?-endotoxin genes including Cry10A gene identified as SEQ ID NO: 12 and Cry11a12 gene identified as SEQ ID NO: 13, transformed in a mono/dicot plant, that are encoded to form a herbicidal and insecticidal toxin proteins in a transgenic plant, resulting in decreased resistance development against insecticidal toxins proteins and increased efficacy against the insect mortality, particularly pink bollworms and army worms. A method or an assay for detecting the presence of transgenic event Kalgin-4 based on the DNA sequence of the recombinant polynucleotide construct inserted into the genome of the transgenic plant and the genomic sequences flanking the insertion site.

Abstract: The present invention refers to the next-generation three genes cotton (Gossypium hirsutum) expressing chloroplast-targeted herbicide tolerant gene resistant to broad and narrow-leaved weedicide sprays and two B. thuringiensis insecticidal genes ?-endotoxin Cry2A and vegetative insecticidal protein gene VIP3A. Both Cry2A and VIP3A genes have different modes of action in controlling a wide spectrum of lepidopteron insect pests, therefore, likely risk of pest resistance will be minimized which is a prevalent problem with single gene Bt cotton expressing Cry1Ac in Pakistan. The invention comprises novel nucleic acid segments encoding proteins comprising herbicide tolerance, Cry2A and VIP3A insecticidal toxins. The polynucleotide segments are revealed, as are Agro-bacterium-mediated transformation vectors holding the nucleic acid segments, plants transformed with claimed segments, methods for transforming plants, and methods for controlling plant infestation by pests.