Abstract: A method includes obtaining, by a first client device a location identifier and an associated inquiry, transmitting wirelessly, by the first client device, the location identifier and the associated inquiry to a server computer, identifying, by the server computer, a second client device, the second client device being currently at a vicinity of a location identified by the location identifier, transmitting wirelessly, by the server computer, the inquiry to the second client device while the second client device is still at the vicinity of the location identified by the location identifier, receiving wirelessly, by the server computer, a response to the inquiry from the second client device, and transmitting wirelessly, by the server computer, the response to the first client device if the response is received within a predetermined period after receiving the inquiry.

Abstract: The present invention provides A microfluidic chip for detecting a nucleic acid, comprising: a reaction chamber configured for amplifying target nucleic acid in a liquid sample to obtain an amplification product; a detection chamber configured for detecting the target nucleic acid in the amplification product; the reaction chamber is in liquid communication with the detection chamber by a first liquid channel, a second channel for receiving the liquid sample including inlet and outlet; the detection chamber is in liquid communication with the second channel by a third channel; wherein air exhaust channel with a first end and a second end, the first end of the exhaust channel is air communication with the detection chamber or/and the reaction chamber, the second end of the exhaust channel is air communication with the second channel.

Abstract: A protein having acrylation activity having a polypeptide sequence derived from SEQ ID NO: 1 or NO:2 by introducing at least one of the following amino acid substitutions: L278A, L278V, W104F, T42A, S47A.

Abstract: A protein having acrylation activity having a polypeptide sequence derived from SEQ ID NO: 1 or NO:2 by introducing at least one of the following amino acid substitutions: L278A, L278V, W104F, T42A, S47A.

Abstract: The present invention relates to nucleic acids that code for triacylglycerol lipases, vectors comprising said nucleic acids, host cells comprising said nucleic acids or vectors, methods for the expression of triacylglycerol lipases in prokaryotes, methods for the detection and for the production of triacylglycerol lipases, triacylglycerol lipases obtainable thereby, and the use of triacylglycerol lipase-encoding nucleic acids, vectors and recombinant host cells for said methods.