Abstract: A pharmaceutical composition comprising a compound of formula I or II and a pharmaceutically acceptable carrier. Methods for treating a proliferative disorder mediated by a methyl transferase comprising administering an anti-proliferative effective amount of the compound of formula I or II are also presented.

Abstract: The present invention provides a general method for identify agents that modulate the activity of histone modifying enzymes, such as an acetylase, deacetylase, methyltransferase, demethylase, kinase, etc. The assay the of invention employs reconstituted, immobilized nucleosomes and fluorescence-based assays, such as fluorescence-based immunoassays, scintillation proximity assays, or FRET assays to determine whether the agent modulates the activity of the histone modifying enzymes.

Abstract: The present invention relates to the modulation of p53 activity based upon the stabilization of p53 via methylation by Set9. Stabilization or destablization of p53 is useful in methods for modulating apoptosis and treating cancer, either alone or in combination with exogenous p53 or antineoplastic agents. Antibodies which specifically recognize methylated p53 are also provided.

Abstract: The present invention is a method for promoting facultative heterochromatin formation using SirT1. Using novel anti-SirT1 and anti-acetylated histone H1 antibodies, human SirT1 protein was shown to interact with and deacetylate histone H1 at lysine 26. Moreover, SirT1 was shown to mediate deactylation of histones H3 and H4 and spreading of hypomethylated H3-K79 with resultant silencing. Using the anti-SirT1 antibody, methods of diagnosing cancer are also provided.

Abstract: The present invention provides an isolated nucleic acid encoding a mammalian capping enzyme. The present invention further provides an isolated nucleic acid encoding a mammalian (Guanine-7-) methyltransferase enzyme. The present invention also provides an isolated mammalian capping enzyme protein or subunit thereof. In addition the present invention provides an isolated mammalian (Guanine-7-) methyltransferase enzyme protein or portion thereof. The present invention further provides methods for catalyzing formation of RNA 5′-terminal GpppN cap complex and for coupled transcription, translation and formation of RNA 5′-terminal GpppN cap complex and methylated RNA 5′-terminal GpppN cap complex. Kits thereto are also provided.