Abstract: A plant squalane composition, a preparation method thereof, and an application thereof are provided. The plant squalane composition includes at least 92.0 wt % of squalane by weight and 0.5-3.0 wt % of cyclosqualane by weight. The preparation method includes hydrogenating a plant squalene raw material, separating the squalane and impurities in a crude plant squalane composition by fractionation, and refining the plant squalane composition. Squalene in the plant squalene raw material accounts for at least 70.0 wt % of a total weight of the plant squalene raw material. A content of the squalane in the plant squalane composition is not less than 92.0 wt %, and a content of the cyclosqualane in the plant squalane composition is 0.5-3.0 wt %. The preparation method solves problems of the prior art that an organic solvent is needed for catalytic hydrogenation, safety and environmental protection are poor, and excessive cyclosqualane is generated in a side reaction.