Abstract: Disclosed in the present application is a composition for removing phospholipids and cell debris, comprising an alcohol, an alcohol ether, and a surfactant, wherein the alcohol accounts for 50-90 wt %, preferably 60-80 wt %; the alcohol ether accounts for 5-40 wt %, preferably 10-20 wt %; the surfactant accounts for 0.1-5 wt %, preferably 1-3 wt %. The present application further provides a method for removing phospholipids and cell debris on biological tissue. The composition of the present application is used for treating biological tissue, and the treated biological tissue has an excellent anti-calcification effect.