Abstract: Disclosed is a LADCP and USBL combined observation device and a use method thereof. The device includes a cable winch system, a mounting frame, a LADCP system, a USBL beacon and a correction system. The LADCP system, the USBL beacon and the correction system can be mounted to the mounting frame by adopting a hardware support platform. The cable winch system can drive the LADCP system to deploy or recover along a vertical section. The LADCP system is used to obtain the current velocity of a single small profile, and the USBL beacon can locate the underwater position information, and the correction system can obtain data information in the seawater where the mounting frame is located, so as to calculate an absolute current velocity according to the velocity obtained by the LADCP system and the current data obtained by the correction system.

Abstract: The present invention discloses a mouse-human chimeric antibody preferably recognizes the MUC1 glycopeptide epitope RPAPGS(GalNAc)TAPPAHG (SEQ ID NO.: 34) on the surface of cancer cells, and the encoding sequences, wherein the monoclonal antibody having a light chain and a heavy chain. Moreover, the present invention provides humanized light and heavy chains, and the encoding sequences. The results of paired expression show that humanized antibodies also recognize the MUC1 glycopeptide epitope RPAPGS(GalNAc)TAPPAHG (SEQ ID NO.: 34) on the surface of cancer cells, and show the same specificity as the parental antibody.

Abstract: The present invention discloses a computational distributed fiber-optic sensing method and system. The method includes: determining a signal source for modulating intensity of incident light, where the signal source is a binary sequence; using an optical pulse sequence obtained after modulation is performed using the signal source, as an incident light signal, and emitting the incident light signal to an optical fiber; acquiring, according to specified sampling frequency, a scattered light signal obtained through optical fiber scattering; determining, according to the incident light signal and the scattered light signal, a time-domain reconstructed image of a to-be-detected light signal by using a time domain-based differential ghost imaging protocol; and determining a sensing signal of the optical fiber according to the time-domain reconstructed image of the to-be-detected light signal.

Abstract: The present invention discloses a computational distributed fiber-optic sensing method and system. The method includes: determining a signal source for modulating intensity of incident light, where the signal source is a binary sequence; using an optical pulse sequence obtained after modulation is performed using the signal source, as an incident light signal, and emitting the incident light signal to an optical fiber; acquiring, according to specified sampling frequency, a scattered light signal obtained through optical fiber scattering; determining, according to the incident light signal and the scattered light signal, a time-domain reconstructed image of a to-be-detected light signal by using a time domain-based differential ghost imaging protocol; and determining a sensing signal of the optical fiber according to the time-domain reconstructed image of the to-be-detected light signal.

Abstract: The present invention discloses a computational distributed fiber-optic sensing method and system. The method includes: determining a signal source for modulating intensity of incident light, where the signal source is a binary sequence; using an optical pulse sequence obtained after modulation is performed using the signal source, as an incident light signal, and emitting the incident light signal to an optical fiber; acquiring, according to specified sampling frequency, a scattered light signal obtained through optical fiber scattering; determining, according to the incident light signal and the scattered light signal, a time-domain reconstructed image of a to-be-detected light signal by using a time domain-based differential ghost imaging protocol; and determining a sensing signal of the optical fiber according to the time-domain reconstructed image of the to-be-detected light signal.

Abstract: The present invention discloses a mouse-human chimeric antibody preferably recognizes the MUC1 glycopeptide epitope RPAPGS(GalNAc)TAPPAHG on the surface of cancer cells, and the encoding sequences, wherein the monoclonal antibody having a light chain and a heavy chain. Moreover, the present invention provides humanized light and heavy chains, and the encoding sequences. The results of paired expression show that humanized antibodies also recognize the MUC1 glycopeptide epitope RPAPGS(GalNAc)TAPPAHG on the surface of cancer cells, and show the same specificity as the parental antibody.

Abstract: A composition for stimulating NKT cells to produce anti-cancer and anti-viral cytokines without causing anergy of NKT cells includes a glycolipid antigen and a nanoparticle conjugated with the glycolipid antigen. The glycolipid antigen and the nanoparticle are not antigenic in mouse and human being. The composition can further include covalent or non-covalent connection between the glycolipid antigen and the nanoparticle. The glycolipid antigen is alpha-galactosylceramide or an analog of that. The nanoparticle can be a polymer. A production method of the composition includes preparing a nanoparticle and a glycolipid antigen and loading the glycolipid antigen to the nanoparticle. The glycolipid antigen can be coated onto the surface of the nanoparticle or encapsulated within the nanoparticle. A method of stimulating NKT cells to produce anti-cancer and anti-viral cytokines without causing anergy of NKT cells is also provided.

Abstract: Nanoconstructs having three components: (1) biodegradable nanopolymers and nanoparticles, (2) immunodrugs such as CpG, and a (3) tumor binding device, which are actively targeted to tumor cells such as melanoma cells through receptor-mediated uptake and methods of using the same are described. Antitumor immunity is further enhanced by combination of PG-CpG nanoconstructs with agonists of positive costimulatory signals and inhibitors of negative immune regulatory signals.

Abstract: A composition for stimulating NKT cells to produce anti-cancer and anti-viral cytokines without causing anergy of NKT cells includes a glycolipid antigen and a nanoparticle conjugated with the glycolipid antigen. The glycolipid antigen and the nanoparticle are not antigenic in mouse and human being. The composition can further include covalent or non-covalent connection between the glycolipid antigen and the nanoparticle. The glycolipid antigen is alpha-galactosylceramide or an analog of that. The nanoparticle can be a polymer. A production method of the composition includes preparing a nanoparticle and a glycolipid antigen and loading the glycolipid antigen to the nanoparticle. The glycolipid antigen can be coated onto the surface of the nanoparticle or encapsulated within the nanoparticle. A method of stimulating NKT cells to produce anti-cancer and anti-viral cytokines without causing anergy of NKT cells is also provided.

Abstract: Provided are methods of activating an NKT cell which include a step of contacting the NKT cell with a sufficient amount of isoglobotrihexosylceramide (iGb3) to induce secretion of a cytokine from the NKT cell, stimulate proliferation of the NKT cell or upregulate expression of a cell surface marker on the NKT cell. Methods of activating an NKT cell population in a subject are also provided.

Abstract: Provided are methods of activating an NKT cell which include a step of contacting the NKT cell with a sufficient amount of isoglobotrihexosylceramide (iGb3) to induce secretion of a cytokine from the NKT cell, stimulate proliferation of the NKT cell or upregulate expression of a cell surface marker on the NKT cell. Methods of activating an NKT cell population in a subject are also provided.

Abstract: Provided are methods of activating an NKT cell which include a step of contacting the NKT cell with a sufficient amount of isoglobotrihexosylceramide (iGb3) to induce secretion of a cytokine from the NKT cell, stimulate proliferation of the NKT cell or upregulate expression of a cell surface marker on the NKT cell. Methods of activating an NKT cell population in a subject are also provided.