Abstract: The present invention relates to the cross-technical fields of medicine, material chemistry, glycobiology and the like, in particular to a sulfonated hyaluronic acid compound, a preparation method therefor and an application thereof. The structural formula of the sulfonated hyaluronic acid compound is shown as in formula (I), wherein R is alkali metal cation or hydrogen; R1, R2, R3 and R4 are respectively independently selected from hydrogen or sulfonate ions and R1, R2, R3 and R4 cannot be hydrogen at the same time, 10<n<4000 and n is an integer. The sulfonated hyaluronic acid compound and the LTBP protein have stronger interaction force, so that a combination of the LTBP and ECM is prevented, so that the mechanical force is insufficient, TGF-? cannot be activated, and fibrosis is inhibited from the source of signal transduction.