Abstract: Disclosed is a system for integrating single cell autofluorescence data with single cell assay data. The system includes a microfluidic chip including a sample inlet for introducing a cell sample, a sample channel including an observation zone, and a single cell autofluorescence spectrometer. The system further includes a removable cell container capable of collecting droplets each containing a single cell exiting the microfluidic chip and further capable of being subjected to a single cell assay. A processor and computer memory receive the autofluorescence data set and associate the autofluorescence data set with single cell assay data.

Abstract: This invention relates, to a method for promoting CNS axon regeneration, comprising (1) inhibiting the expression or activity in a neuron of one or more of the members of the Krüppel-like transcription factor (KLF) family that suppress axon growth (e.g., KLF 1, 2, 3, 4, 5, 9,12, 13, 14, 15 and/or 16), and/or (2) stimulating the expression or activity in a neuron of one or more of the members of the KLF family that promote axon growth (e.g., KLF 6 and/or 7).

Abstract: This invention relates, e.g., to a method for promoting CNS axon regeneration, comprising (1) inhibiting the expression or activity in a neuron of one or more of the members of the Krüppel-like transcription factor (KLF) family that suppress axon growth (e.g., KLF 1, 2, 3, 4, 5, 9, 12, 13, 14, 15 and/or 16), and/or (2) stimulating the expression or activity in a neuron of one or more of the members of the KLF family that promote axon growth (e.g., KLF 6 and/or 7).