Abstract: The present invention provides multiple polynucleotide sequences from the same novel gene, the exons comprising the polynucleotide sequences, and the proteins encoded by the polynucleotide sequences. Three splicing variant polynucleotides were isolated from prostate tissue. The polypeptides, including the splicing variants, have a region of hydrophobicity indicative of a transmembrane domain and all three extracellular and cytoplasmic domains.

Abstract: A method is provided for the rapid identification of protein-protein interaction networks within a cell, tissue, or whole genome. The introduction of a multi-bait approach is a distinguishing feature of the technology. In this method a pair of two-hybrid cDNA libraries, each one carrying the complement of genes from the tissue under study, are combined for an interaction screen. A large number of yeast colonies, each identifying a protein interaction pair, are picked and distributed in single wells, providing an arrayed archive of protein-protein interactions. The archive also serves as a source of plasmids to construct arrayed replicas containing DNA of the interacting plasmid pairs. Hybridization of a given cDNA to the arrayed replicas identifies the corresponding interacting clones. Protein interaction networks are constructed by iteration of the hybridization with newly identified interacting clones.