Patents by Inventor Darrel W. Stafford

Darrel W. Stafford has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • METHODS AND COMPOSITIONS FOR PRODUCING VITAMIN K DEPENDENT PROTEINS
    Publication number: 20240101973
    Abstract: The present invention relates to methods and compositions for improving the productivity of recombinant vitamin K dependent protein expression in host cells.
    Type: Application
    Filed: March 15, 2023
    Publication date: March 28, 2024
    Inventors: Darrel W. Stafford, Tao Li
  • METHOD OF PRODUCING BIOLOGICALLY ACTIVE VITAMIN K DEPENDENT PROTEINS BY RECOMBINANT METHODS
    Publication number: 20230287471
    Abstract: The invention relates to commercially viable methods for producing biologically active vitamin K dependent proteins, particularly Factor IX. Factor IX is produced at a level of at least about 15 mg/L and is at least 25% biologically active. The method relies upon co-expression of one or more of paired basic amino acid converting enzyme (PACE), vitamin K dependent epoxide reductase (VKOR) and vitamin K dependent ?-glutamyl carboxylase (VKGC) at a preferred ratio so that the vitamin K dependent protein is efficiently produced and processed by a recombinant cell.
    Type: Application
    Filed: October 20, 2022
    Publication date: September 14, 2023
    Inventors: William N. Drohan, Michael J. Griffith, John R. Taylor, Darrel W. Stafford
  • METHODS AND COMPOSITIONS FOR PRODUCING VITAMIN K DEPENDENT PROTEINS
    Publication number: 20220228131
    Abstract: The present invention relates to methods and compositions for improving the productivity of recombinant vitamin K dependent protein expression in host cells.
    Type: Application
    Filed: August 26, 2021
    Publication date: July 21, 2022
    Inventors: Darrel W. Stafford, Tao Li
  • METHODS AND COMPOSITIONS FOR PRODUCING ACTIVE VITAMIN K-DEPENDENT PROTEINS
    Publication number: 20220177856
    Abstract: The present invention provides a stable cell line overexpressing a recombinant vitamin K-dependent (VKD) protein and both a recombinant vitamin K epoxide reductase (VKOR) and a recombinant vitamin K dependent gamma carboxylase (VKGC), wherein the amount of fully carboxylated VKD protein produced in the cell is increased 10% as compared to the amount of fully carboxylated VKD protein produced in the cell in the absence of the recombinant VKOR and the recombinant VKGC.
    Type: Application
    Filed: July 16, 2021
    Publication date: June 9, 2022
    Inventors: Darrel W. Stafford, Tao Li
  • METHODS AND COMPOSITIONS FOR MODIFIED FACTOR IX PROTEINS
    Publication number: 20220073894
    Abstract: Factor IX proteins are described with an increase in the number of glycosylation sites and other modifications to provide Factor IX proteins that have higher specific activity and a longer useful clotting function relative to wild type or non-modified Factor IX protein.
    Type: Application
    Filed: April 16, 2021
    Publication date: March 10, 2022
    Inventors: Darrel W. Stafford, Dengmin Feng
  • METHODS AND COMPOSITIONS FOR PRODUCING ACTIVE VITAMIN K-DEPENDENT PROTEINS
    Publication number: 20210332333
    Abstract: The present invention provides a stable cell line overexpressing a recombinant vitamin K-dependent (VKD) protein and both a recombinant vitamin K epoxide reductase (VKOR) and a recombinant vitamin K dependent gamma carboxylase (VKGC), wherein the amount of fully carboxylated VKD protein produced in the cell is increased 10% as compared to the amount of fully carboxylated VKD protein produced in the cell in the absence of the recombinant VKOR and the recombinant VKGC.
    Type: Application
    Filed: November 18, 2020
    Publication date: October 28, 2021
    Inventors: Darrel W. Stafford, Tao Li
  • METHODS AND COMPOSITIONS FOR PRODUCING VITAMIN K DEPENDENT PROTEINS
    Publication number: 20210130795
    Abstract: The present invention relates to methods and compositions for improving the productivity of recombinant vitamin K dependent protein expression in host cells.
    Type: Application
    Filed: May 8, 2020
    Publication date: May 6, 2021
    Inventors: Darrel W. Stafford, Tao Li
  • METHOD OF PRODUCING BIOLOGICALLY ACTIVE VITAMIN K DEPENDENT PROTEINS BY RECOMBINANT METHODS
    Publication number: 20210095323
    Abstract: The invention relates to commercially viable methods for producing biologically active vitamin K dependent protein, particularly Factor IX. Factor IX is produced at a level of at least about 15 mg/L and is at least 25% biologically active. The method relies upon co-expression of one or more of paired basic amino acid converting enzyme (PACE), vitamin K dependent epoxide reductase (VKOR) and vitamin K dependent ?-glutamyl carboxylase (VKGC) at a preferred ratio so that the vitamin K dependent protein is efficiently produced and processed by a recombinant cell.
    Type: Application
    Filed: June 2, 2020
    Publication date: April 1, 2021
    Inventors: William N. Drohan, Michael J. Griffith, John R. Taylor, Darrel W. Stafford
  • METHODS AND COMPOSITIONS FOR MODIFIED FACTOR IX PROTEINS
    Publication number: 20200362328
    Abstract: Factor IX proteins are described with an increase in the number of glycosylation sites and other modifications to provide Factor IX proteins that have higher specific activity and a longer useful clotting function relative to wild type or non-modified Factor IX protein.
    Type: Application
    Filed: December 17, 2019
    Publication date: November 19, 2020
    Inventors: Darrel W. Stafford, Dengmin Feng
  • METHODS AND COMPOSITIONS FOR PRODUCING VITAMIN K DEPENDENT PROTEINS
    Publication number: 20200263148
    Abstract: The present invention relates to methods and compositions for improving the productivity of recombinant vitamin K dependent protein expression in host cells.
    Type: Application
    Filed: September 18, 2019
    Publication date: August 20, 2020
    Inventors: Darrel W. Stafford, Tao Li
  • METHODS AND COMPOSITIONS FOR PRODUCING ACTIVE VITAMIN K-DEPENDENT PROTEINS
    Publication number: 20200224177
    Abstract: The present invention provides a stable cell line overexpressing a recombinant vitamin K-dependent (VKD) protein and both a recombinant vitamin K epoxide reductase (VKOR) and a recombinant vitamin K dependent gamma carboxylase (VKGC), wherein the amount of fully carboxylated VKD protein produced in the cell is increased 10% as compared to the amount of fully carboxylated VKD protein produced in the cell in the absence of the recombinant VKOR and the recombinant VKGC.
    Type: Application
    Filed: August 27, 2019
    Publication date: July 16, 2020
    Inventors: Darrel W. Stafford, Tao Li
  • METHODS AND COMPOSITIONS FOR MODIFIED FACTOR IX PROTEINS
    Publication number: 20200095565
    Abstract: Factor IX proteins are described with an increase in the number of glycosylation sites and other modifications to provide Factor IX proteins that have higher specific activity and a longer useful clotting function relative to wild type or non-modified Factor IX protein.
    Type: Application
    Filed: May 2, 2019
    Publication date: March 26, 2020
    Inventors: Darrel W. Stafford, Dengmin Feng
  • METHODS AND COMPOSITIONS FOR PRODUCING VITAMIN K DEPENDENT PROTEINS
    Publication number: 20200002684
    Abstract: The present invention relates to methods and compositions for improving the productivity of recombinant vitamin K dependent protein expression in host cells.
    Type: Application
    Filed: February 11, 2019
    Publication date: January 2, 2020
    Inventors: Darrel W. Stafford, Tao Li
  • METHODS AND COMPOSITIONS FOR PRODUCING ACTIVE VITAMIN K-DEPENDENT PROTEINS
    Publication number: 20190367888
    Abstract: The present invention provides a stable cell line overexpressing a recombinant vitamin K-dependent (VKD) protein and both a recombinant vitamin K epoxide reductase (VKOR) and a recombinant vitamin K dependent gamma carboxylase (VKGC), wherein the amount of fully carboxylated VKD protein produced in the cell is increased 10% as compared to the amount of fully carboxylated VKD protein produced in the cell in the absence of the recombinant VKOR and the recombinant VKGC.
    Type: Application
    Filed: January 18, 2019
    Publication date: December 5, 2019
    Inventors: Darrel W. Stafford, Tao Li
  • METHODS AND COMPOSITIONS FOR MODIFIED FACTOR IX PROTEINS
    Publication number: 20190249164
    Abstract: Factor IX proteins are described with an increase in the number of glycosylation sites and other modifications to provide Factor IX proteins that have higher specific activity and a longer useful clotting function relative to wild type or non-modified Factor IX protein.
    Type: Application
    Filed: September 20, 2018
    Publication date: August 15, 2019
    Inventors: Darrel W. Stafford, Dengmin Feng
  • METHODS AND COMPOSITIONS FOR PRODUCING VITAMIN K DEPENDENT PROTEINS
    Publication number: 20190153402
    Abstract: The present invention relates to methods and compositions for improving the productivity of recombinant vitamin K dependent protein expression in host cells.
    Type: Application
    Filed: June 27, 2018
    Publication date: May 23, 2019
    Inventors: Darrel W. Stafford, Tao Li
  • METHOD OF PRODUCING BIOLOGICALLY ACTIVE VITAMIN K DEPENDENT PROTEINS BY RECOMBINANT METHODS
    Publication number: 20190032101
    Abstract: The invention relates to commercially viable methods for producing biologically active vitamin R dependent proteins, particularly Factor IX. Factor IX is produced at a level of at least about 15 mg/L and is at least 25% biologically active. The method relies upon co-expression of one or more of paired basic amino acid converting enzyme (PACE), vitamin K dependent epoxide reductase (VKOR) and vitamin K dependent ?-glutamyl carboxylase (VKGC) at a preferred ratio so that the vitamin K dependent protein is efficiently produced and processed by a recombinant cell.
    Type: Application
    Filed: January 19, 2018
    Publication date: January 31, 2019
    Inventors: William N. Drohan, Michael J. Griffith, John R. Taylor, Darrel W. Stafford
  • METHODS AND COMPOSITIONS FOR PRODUCING ACTIVE VITAMIN K-DEPENDENT PROTEINS
    Publication number: 20180334658
    Abstract: The present invention provides a stable cell line overexpressing a recombinant vitamin K-dependent (VKD) protein and both a recombinant vitamin K epoxide reductase (VKOR) and a recombinant vitamin K dependent gamma carboxylase (VKGC), wherein the amount of fully carboxylated VKD protein produced in the cell is increased 10% as compared to the amount of fully carboxylated VKD protein produced in the cell in the absence of the recombinant VKOR and the recombinant VKGC.
    Type: Application
    Filed: October 26, 2017
    Publication date: November 22, 2018
    Inventors: Darrel W. Stafford, Tao Li
  • METHODS AND COMPOSITIONS FOR PRODUCING VITAMIN K DEPENDENT PROTEINS
    Publication number: 20180298350
    Abstract: The present invention relates to methods and compositions for improving the productivity of recombinant vitamin K dependent protein expression in host cells.
    Type: Application
    Filed: October 26, 2017
    Publication date: October 18, 2018
    Inventors: Darrel W. Stafford, Tao Li
  • Methods and compositions for producing active vitamin K-dependent proteins
    Patent number: 9828588
    Abstract: The present invention provides a stable cell line overexpressing a recombinant vitamin K-dependent (VKD) protein and both a recombinant vitamin K epoxide reductase (VKOR) and a recombinant vitamin K dependent gamma carboxylase (VKGC), wherein the amount of fully carboxylated VKD protein produced in the cell is increased 10% as compared to the amount of fully carboxylated VKD protein produced in the cell in the absence of the recombinant VKOR and the recombinant VKGC.
    Type: Grant
    Filed: September 25, 2014
    Date of Patent: November 28, 2017
    Assignee: The University of North Carolina at Chapel Hill
    Inventors: Darrel W. Stafford, Tao Li