Patents by Inventor Darren Link
Darren Link has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11534727Abstract: The present invention generally relates to droplet libraries and to systems and methods for the formation of libraries of droplets. The present invention also relates to methods utilizing these droplet libraries in various biological, chemical, or diagnostic assays.Type: GrantFiled: October 11, 2016Date of Patent: December 27, 2022Assignee: Bio-Rad Laboratories, Inc.Inventors: Darren Link, John Brian Hutchison, Michael Samuels, Michael Weiner
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Publication number: 20220143617Abstract: The invention provides methods for assessing one or more predetermined characteristics or properties of a microfluidic droplet within a microfluidic channel, and regulating one or more fluid flow rates within that channel to selectively alter the predetermined microdroplet characteristic or property using a feedback control.Type: ApplicationFiled: January 24, 2022Publication date: May 12, 2022Inventors: Benjamin Miller, Brian Hutchison, Andrew Wilson, Jonathan William Larson, Qun Zhong, Yevgeny Yurkovetsky, Darren Link, Mark Weary
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Patent number: 10837883Abstract: The present invention generally relates to systems and methods to create stable emulsions with low rates of exchange of molecules between microdroplets.Type: GrantFiled: December 22, 2010Date of Patent: November 17, 2020Assignees: Bio-Rad Laboratories, Inc., University of StrasbourgInventors: Felix Kleinschmidt, Andrew David Griffiths, François Caron, Abdeslam El Harrak, Eric Brouzes, Darren Link
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Patent number: 9925504Abstract: The invention describes a method for the synthesis of compounds comprising the steps of: (a) compartmentalizing two or more sets of primary compounds into microcapsules; such that a proportion of the microcapsules contains two or more compounds; and (b) forming secondary compounds in the microcapsules by chemical reactions between primary compounds from different sets; wherein one or both of steps (a) and (b) is performed under microfluidic control; preferably electronic microfluidic control The invention further allows for the identification of compounds which bind to a target component of a biochemical system or modulate the activity of the target, and which is co-compartmentalized into the microcapsules.Type: GrantFiled: December 20, 2006Date of Patent: March 27, 2018Assignees: President and Fellows of Harvard College, Medical Research CouncilInventors: Andrew Griffiths, David Weitz, Keunho Ahn, Darren Link, Jerome Bibette
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Patent number: 9839890Abstract: The invention describes a method for the synthesis of compounds comprising the steps of: (a) compartmentalising two or more sets of primary compounds into microcapsules; such that a proportion of the microcapsules contains two or more compounds; and (b) forming secondary compounds in the microcapsules by chemical reactions between primary compounds from different sets; wherein one or both of steps (a) and (b) is performed under microfluidic control; preferably electronic microfluidic control, The invention further allows for the identification of compounds which bind to a target component of a biochemical system or modulate the activity of the target, and which is co-compartmentalised into the microcapsules.Type: GrantFiled: October 12, 2005Date of Patent: December 12, 2017Assignees: National Science Foundation, Medical Research Council, President and Fellows of Harvard CollegeInventors: Andrew Griffiths, David Weitz, Darren Link, Keunho Ahn, Jerome Bibette
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Patent number: 9733168Abstract: The invention provides methods for assessing one or more predetermined characteristics or properties of a microfluidic droplet within a microfluidic channel, and regulating one or more fluid flow rates within that channel to selectively alter the predetermined microdroplet characteristic or property using a feedback control.Type: GrantFiled: March 28, 2016Date of Patent: August 15, 2017Assignee: Raindance Technologies, Inc.Inventors: Benjamin Miller, Brian Hutchison, Andrew Wilson, Jonathan William Larson, Qun Zhong, Yevgeny Yurkovetsky, Darren Link, Mark Weary
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Publication number: 20170028365Abstract: The present invention generally relates to droplet libraries and to systems and methods for the formation of libraries of droplets. The present invention also relates to methods utilizing these droplet libraries in various biological, chemical, or diagnostic assays.Type: ApplicationFiled: October 11, 2016Publication date: February 2, 2017Inventors: Darren Link, Brian Hutchison, Michael Samuels, Michael Weiner
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Patent number: 9556470Abstract: The invention generally relates to methods for quantifying an amount of enzyme molecules. Systems and methods of the invention are provided for measuring an amount of target by forming a plurality of fluid partitions, a subset of which include the target, performing an enzyme-catalyzed reaction in the subset, and detecting the number of partitions in the subset. The amount of target can be determined based on the detected number.Type: GrantFiled: June 1, 2012Date of Patent: January 31, 2017Assignee: Raindance Technologies, Inc.Inventors: Darren Link, Michael L. Samuels
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Publication number: 20160209303Abstract: The invention provides methods for assessing one or more predetermined characteristics or properties of a microfluidic droplet within a microfluidic channel, and regulating one or more fluid flow rates within that channel to selectively alter the predetermined microdroplet characteristic or property using a feedback control.Type: ApplicationFiled: March 28, 2016Publication date: July 21, 2016Inventors: Benjamin Miller, Brian Hutchison, Andrew Wilson, Jonathan William Larson, Qun Zhong, Yevgeny Yurkovetsky, Darren Link, Mark Weary
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Patent number: 9328344Abstract: The present invention provides novel microfluidic devices and methods that are useful for performing high-throughput screening assays and combinatorial chemistry. Such methods can include labeling a library of compounds by emulsifying aqueous solutions of the compounds and aqueous solutions of unique liquid labels on a microfluidic device, which includes a plurality of electrically addressable, channel bearing fluidic modules integrally arranged on a microfabricated substrate such that a continuous channel is provided for flow of immiscible fluids, whereby each compound is labeled with a unique liquid label, pooling the labeled emulsions, coalescing the labeled emulsions with emulsions containing a specific cell or enzyme, thereby forming a nanoreactor, screening the nanoreactors for a desirable reaction between the contents of the nanoreactor, and decoding the liquid label, thereby identifying a single compound from a library of compounds.Type: GrantFiled: February 5, 2013Date of Patent: May 3, 2016Assignee: Raindance Technologies, Inc.Inventors: Darren Link, Laurent Boitard, Jeffrey Branciforte, Yves Charles, Gilbert Feke, John Q. Lu, David Marran, Ahmadali Tabatabai, Michael Weiner, Wolfgang Hinz, Jonathan M. Rothberg
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Patent number: 9029083Abstract: The invention describes a method for isolating one or more genetic elements encoding a gene product having a desired activity, comprising the steps of: (a) compartmentalising genetic elements into microcapsules; and (b) sorting the genetic elements which express the gene product having the desired activity; wherein at least one step is under microfluidic control. The invention enables the in vitro evolution of nucleic acids and proteins by repeated mutagenesis and iterative applications of the method of the invention.Type: GrantFiled: October 10, 2005Date of Patent: May 12, 2015Assignees: Medical Research Council, President and Fellows of Harvard CollegeInventors: Andrew David Griffiths, David Weitz, Darren Link, Keunho Ahn, Jerome Bibette
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Patent number: 8857462Abstract: The invention provides methods for assessing one or more predetermined characteristics or properties of a microfluidic droplet within a microfluidic channel, and regulating one or more fluid flow rates within that channel to selectively alter the predetermined microdroplet characteristic or property using a feedback control.Type: GrantFiled: September 9, 2013Date of Patent: October 14, 2014Assignee: Raindance Technologies, Inc.Inventors: Benjamin Miller, Brian Hutchison, Andrew Wilson, Jonathan Larson, Qun Zhong, Yevgeny Yurkovetsky, Darren Link, Mark Weary
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Publication number: 20140295421Abstract: The invention generally relates to methods for quantifying an amount of enzyme molecules. Systems and methods of the invention are provided for measuring an amount of target by forming a plurality of fluid partitions, a subset of which include the target, performing an enzyme-catalyzed reaction in the subset, and detecting the number of partitions in the subset. The amount of target can be determined based on the detected number.Type: ApplicationFiled: June 1, 2012Publication date: October 2, 2014Applicant: RAINDANCE TECHNOLOGIES, INC.Inventors: Darren Link, Michael L. Samuels
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Patent number: 8841071Abstract: The invention generally relates to methods for sample multiplexing. In certain embodiments, methods of the invention obtaining a plurality of different reactant molecules, attaching a unique identifier to the reactant molecules, and forming a droplet including the reactant molecules.Type: GrantFiled: May 31, 2012Date of Patent: September 23, 2014Assignee: Raindance Technologies, Inc.Inventor: Darren Link
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Publication number: 20140076430Abstract: The invention provides methods for assessing one or more predetermined characteristics or properties of a microfluidic droplet within a microfluidic channel, and regulating one or more fluid flow rates within that channel to selectively alter the predetermined microdroplet characteristic or property using a feedback control.Type: ApplicationFiled: September 9, 2013Publication date: March 20, 2014Applicant: RAINDANCE TECHNOLOGIES, INC.Inventors: Benjamin Miller, Brian Hutchison, Andrew Wilson, Jonathan Larson, Qun Zhong, Yevgeny Yurkovetsky, Darren Link, Mark Weary
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Patent number: 8528589Abstract: The invention provides methods for assessing one or more predetermined characteristics or properties of a microfluidic droplet within a microfluidic channel, and regulating one or more fluid flow rates within that channel to selectively alter the predetermined microdroplet characteristic or property using a feedback control.Type: GrantFiled: March 23, 2010Date of Patent: September 10, 2013Assignee: Raindance Technologies, Inc.Inventors: Benjamin Miller, Brian Hutchison, Andrew Wilson, Jonathan Larson, Qun Zhong, Yevgeny Yurkovetsky, Darren Link, Mark Weary
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Publication number: 20130217583Abstract: The present invention provides novel microfluidic devices and methods that are useful for performing high-throughput screening assays and combinatorial chemistry. Such methods can include labeling a library of compounds by emulsifying aqueous solutions of the compounds and aqueous solutions of unique liquid labels on a microfluidic device, which includes a plurality of electrically addressable, channel bearing fluidic modules integrally arranged on a microfabricated substrate such that a continuous channel is provided for flow of immiscible fluids, whereby each compound is labeled with a unique liquid label, pooling the labeled emulsions, coalescing the labeled emulsions with emulsions containing a specific cell or enzyme, thereby forming a nanoreactor, screening the nanoreactors for a desirable reaction between the contents of the nanoreactor, and decoding the liquid label, thereby identifying a single compound from a library of compounds.Type: ApplicationFiled: February 5, 2013Publication date: August 22, 2013Inventors: Darren Link, Laurent Boitard, Jeffrey Branciforte, Yves Charles, Gilbert Feke, John Q. Lu, David Marran, Ahmadali Tabatabai, Michael Weiner, Wolfgang Hinz, Jonathan M. Rothberg
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Publication number: 20130178368Abstract: The invention describes a method for isolating one or more genetic elements encoding a gene product having a desired activity, comprising the steps of: (a) compartmentalising genetic elements into microcapsules; and (b) sorting the genetic elements which express the gene product having the desired activity; wherein at least one step is under microfluidic control. The invention enables the in vitro evolution of nucleic acids and proteins by repeated mutagenesis and iterative applications of the method of the invention.Type: ApplicationFiled: December 5, 2012Publication date: July 11, 2013Inventors: Andrew David Griffiths, David Weitz, Darren Link, Keunho Ahn, Jerome Bibette
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Publication number: 20130157872Abstract: The invention describes a method for isolating one or more genetic elements encoding a gene product having a desired activity, comprising the steps of: (a) compartmentalising genetic elements into microcapsules; and (b) sorting the genetic elements which express the gene product having the desired activity; wherein at least one step is under microfluidic control. The invention enables the in vitro evolution of nucleic acids and proteins by repeated mutagenesis and iterative applications of the method of the invention.Type: ApplicationFiled: December 3, 2012Publication date: June 20, 2013Inventors: Andrew David Griffiths, David Weitz, Darren Link, Keunho Ahn, Jerome Bibette
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Publication number: 20130109575Abstract: The present invention generally relates to systems and methods to create stable emulsions with low rates of exchange of molecules between microdroplets.Type: ApplicationFiled: December 22, 2010Publication date: May 2, 2013Applicant: RAINDANCE TECHNOLOGIES, INC.Inventors: Felix Kleinschmidt, Andrew David Griffiths, François Caron, Abdeslam El Harrak, Eric Brouzes, Darren Link