Abstract: A sample processing module includes a PCR assembly including at least one PCR reaction vial. A sample assembly is configured to hold a fluid sample therein, said sample assembly being attachable to said PCR assembly so as to form a closed system. A delivery channel is formed in said PCR assembly, said delivery channel being in fluidic communication with said PCR reaction vial and with said sample assembly. A pressurization device, said pressurization device being configured to increase a pressure of said fluid sample in said sample assembly and direct at least a portion said fluid sample through said delivery channel to said PCR reaction vial. A chemically inert magnetically responsive bead is disposed within the PCR reaction vial. A system is provided for oscillating for altering a position of the magnetically responsive bead within the PCR reaction vial.

Abstract: Methods and apparatus for use in connection with the performance of the polymerase chain reaction are provided. An exemplary sample processing module is described that includes a sample assembly and a PCR assembly, the sample processing module being configured to hold the sample therein at a pressure higher than ambient pressure. A sample is added to the sample assembly at the time of use, which is then connected to the PCR assembly. Embodiments of the cartridge include a flow restriction device that enables or aids in creating a higher pressure within the reaction vial. The sample is introduced into a PCR reaction vial, which contains all of the constituents of a PCR reaction mixture that are necessary to process the sample and provide amplified DNA of interest, if that DNA was present in the sample.

Abstract: Methods and apparatus for use in connection with the performance of the polymerase chain reaction are provided. An exemplary sample processing module is described that includes a sample assembly and a PCR assembly, the sample processing module being configured to hold the sample therein at a pressure higher than ambient pressure. A sample is added to the sample assembly at the time of use, which is then connected to the PCR assembly. Embodiments of the cartridge include a flow restriction device that enables or aids in creating a higher pressure within the reaction vial. The sample is introduced into a PCR reaction vial, which contains all of the constituents of a PCR reaction mixture that are necessary to process the sample and provide amplified DNA of interest, if that DNA was present in the sample.