Patents by Inventor Darryl W. Brousmiche

Darryl W. Brousmiche has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20230013977
    Abstract: The present disclosure relates to a method for using chemical tags which have two or more sites for ionization to improve quantification and identification of components of interest from a complex mixture. This method relies on first selectively reacting one or more component in a sample with a chemical tag having two or more sites for ionization, followed by separation of components based on charge status, and finally characterization of each component to identify the same. Additionally disclosed are compounds useful as chemical tags in the disclosed methods.
    Type: Application
    Filed: August 30, 2022
    Publication date: January 19, 2023
    Applicant: Waters Technologies Corporation
    Inventors: Giuseppe Astarita, Darryl W. Brousmiche
  • Publication number: 20220412963
    Abstract: Novel reagents comprising MS active, fluorescent compounds having an activated functionality for reaction with aldehydes and useful in labeling biomolecules such as glycans and methods of making the same are taught and described.
    Type: Application
    Filed: June 3, 2021
    Publication date: December 29, 2022
    Inventors: Darryl W. Brousmiche, Matthew A. Lauber
  • Publication number: 20220381788
    Abstract: Methods for derivatization of biomolecules including glycans or other biopolymers with one or more fluorescent, MS active compounds by reductive amination or rapid tagging in order to produce derivatized glycan having a pKa >7 and between about 200 ? and about 1000 ? of nonpolar surface area are described.
    Type: Application
    Filed: May 2, 2022
    Publication date: December 1, 2022
    Applicant: Waters Technologies Corporation
    Inventors: Darryl W. Brousmiche, Matthew A. Lauber
  • Publication number: 20220362741
    Abstract: In some aspects, the present disclosure pertains to chromatographic materials that comprise (a) a bulk material and (b) a zwitterionic polymer covalently linked to a surface of the bulk material, in which the zwitterionic polymer comprises one or more monomer residues that comprise an amide or urea moiety, a positively charged moiety, and a negatively charged moiety. Other aspects of the present disclosure pertain to chromatographic separation devices that comprise such chromatographic materials, to chromatographic methods that employ such chromatographic separation devices, and to kits that contain (i) such chromatographic materials and (ii) one or more chromatographic devices for containing such materials.
    Type: Application
    Filed: April 28, 2022
    Publication date: November 17, 2022
    Applicant: Waters Technologies Corporation
    Inventors: Nicole L. Lawrence, Darryl W. Brousmiche, Jessica Field, Daniel P. Walsh
  • Patent number: 11460438
    Abstract: The present disclosure relates to a method for using chemical tags which have two or more sites for ionization to improve quantification and identification of components of interest from a complex mixture. This method relies on first selectively reacting one or more component in a sample with a chemical tag having two or more sites for ionization, followed by separation of components based on charge status, and finally characterization of each component to identify the same. Additionally disclosed are compounds useful as chemical tags in the disclosed methods.
    Type: Grant
    Filed: February 17, 2017
    Date of Patent: October 4, 2022
    Assignee: Waters Technologies Corporation
    Inventors: Giuseppe Astarita, Darryl W. Brousmiche
  • Patent number: 11448652
    Abstract: Reagents comprising MS active, fluorescent molecules with an activated functionality for reaction with amines useful in tagging biomolecules such as N-glycans and uses thereof are taught and described. In particular, compounds for use as a reagent for rapid fluorescence tagging of biomolecules and enhanced MS signaling are provided. The compounds may have optical centers and therefore may occur in different enantiomeric and diastereomeric configurations. These MS active, fluorescent compounds may have three functional components: (a) a tertiary amino group or other MS active atom; (b) a highly fluorescent moiety, and (c) a reactive group that rapidly reacts with amines. The reactive group provides rapid tagging of desired bio-molecules. The fluorescent moiety provides the fluorescent signal. The tertiary amino group provides the MS signal.
    Type: Grant
    Filed: September 5, 2019
    Date of Patent: September 20, 2022
    Assignee: WATERS TECHNOLOGIES CORPORATION
    Inventors: Darryl W. Brousmiche, Ying-Qing Yu, Matthew Lauber
  • Publication number: 20220280909
    Abstract: Novel sorbents, devices, kits and methods useful for sample treatment are disclosed herein.
    Type: Application
    Filed: May 23, 2022
    Publication date: September 8, 2022
    Applicant: Waters Technologies Corporation
    Inventors: Darryl W. Brousmiche, Kevin D. Wyndham, Nicole L. Lawrence, Jacob N. Fairchild, Bonnie Alden
  • Publication number: 20220268779
    Abstract: Methods of analyzing glycosylated biomolecules include the steps of producing a deglycosylation mixture of biomolecules deglycosylated by natural or synthetic enzymatic or chemical techniques; providing a reagent solution having a labeling reagent in a polar aprotic, non-nucleophilic organic solvent; and mixing the deglycosylation mixture with the reagent solution in an excess of labeling reagent to produce derivatized glycosylamines. The method steps can be carried out purposefully without depletion of protein matter. A quenching solution can be added to the reaction mixture so that the pH of the reaction mixture is shifted to above 10. The yield of derivatized glycosylamines can be in an amount of about 80 to about 100 mole percent of the reaction mixture with minimal overlabeling, less than 0.2 mole percent.
    Type: Application
    Filed: May 11, 2022
    Publication date: August 25, 2022
    Applicant: Waters Technologies Corporation
    Inventors: Matthew A. Lauber, Darryl W. Brousmiche, Stephan M. Koza
  • Publication number: 20220252553
    Abstract: This invention relates to encapsulated reagents for sample and workflow preparation prior to chromatographic, spectroscopic or other analytical systems, use thereof, and devices comprising the same.
    Type: Application
    Filed: February 17, 2022
    Publication date: August 11, 2022
    Applicant: Waters Technoligies Corporation
    Inventors: Kevin Daniel Wyndham, Darryl W. Brousmiche
  • Patent number: 11376561
    Abstract: Sorbents, devices, kits and methods useful for sample treatment are disclosed herein. In particular embodiments, described are inorganic/organic hybrid sorbent particles comprising (a) a core region that comprises a silica component and (b) a surface region that comprises an organic copolymer comprising at least one hydrophobic organic monomer and at least one hydrophilic organic monomer.
    Type: Grant
    Filed: October 2, 2019
    Date of Patent: July 5, 2022
    Assignee: Waters Technologies Corporation
    Inventors: Darryl W. Brousmiche, Kevin D. Wyndham, Nicole L. Lawrence, Jacob N. Fairchild, Bonnie A. Alden
  • Patent number: 11371996
    Abstract: Methods of analyzing glycosylated biomolecules include the steps of producing a deglycosylation mixture of biomolecules deglycosylated by natural or synthetic enzymatic or chemical techniques; providing a reagent solution having a labeling reagent in a polar aprotic, non-nucleophilic organic solvent; and mixing the deglycosylation mixture with the reagent solution in an excess of labeling reagent to produce derivatized glycosylamines. The method steps can be carried out purposefully without depletion of protein matter. A quenching solution can be added to the reaction mixture so that the pH of the reaction mixture is shifted to above 10. The yield of derivatized glycosylamines can be in an amount of about 80 to about 100 mole percent of the reaction mixture with minimal overlabeling, less than 0.2 mole percent.
    Type: Grant
    Filed: October 28, 2015
    Date of Patent: June 28, 2022
    Assignee: Waters Technologies Corporation
    Inventors: Matthew A. Lauber, Darryl W. Brousmiche, Stephan M. Koza
  • Patent number: 11353461
    Abstract: Methods for derivatization of biomolecules including glycans or other biopolymers with one or more fluorescent, MS active compounds by reductive amination or rapid tagging in order to produce derivatized glycan having a pKa>7 and between about 200 ?2 and about 1000 ?2 of nonpolar surface area are described.
    Type: Grant
    Filed: June 19, 2017
    Date of Patent: June 7, 2022
    Assignee: Waters Technologies Corporation
    Inventors: Darryl W. Brousmiche, Matthew A. Lauber
  • Patent number: 11352325
    Abstract: Reagents comprising MS active, fluorescent molecules with an activated functionality for reaction with amines useful in tagging biomolecules such as N-glycans and uses thereof are taught and described. In particular embodiments, the MS active, fluorescent molecules are of the compound of the Formula III or of the Formula IV where R1, R2 and R3 are defined herein.
    Type: Grant
    Filed: January 25, 2016
    Date of Patent: June 7, 2022
    Assignee: Waters Technologies Corporation
    Inventors: Darryl W. Brousmiche, Matthew A. Lauber
  • Publication number: 20220145389
    Abstract: The present disclosure relates to compositions that comprise a fluorescent and/or MS-active nucleic acid probe that comprises (a) a nucleic-acid-based moiety and (b) a fluorescent moiety comprising a fluorophore, an MS-active moiety, or both a fluorescent moiety and an MS-active moiety, linked to the nucleic-acid-based moiety, among other aspects.
    Type: Application
    Filed: November 4, 2021
    Publication date: May 12, 2022
    Inventors: Armand Gatien Ngounou Wetie, Mark D. Wrona, Darryl W. Brousmiche
  • Publication number: 20220118443
    Abstract: The present disclosure is directed to a coating process for chromatographic surfaces. Embodiments of the present disclosure feature a two-step, vapor-liquid phase organosilane deposition method for creating a hydrophilic, non-ionic surface in a chromatographic system.
    Type: Application
    Filed: October 15, 2021
    Publication date: April 21, 2022
    Applicant: Waters Technologies Corporation
    Inventors: Mathew H. DeLano, Matthew Lauber, Andrew Wyatt Schmudlach, Daniel P. Walsh, Yeliz Tunc Sarisozen, Jonathan Belanger, Darryl W. Brousmiche, Andrew Bates-Harrison, Nicole L. Lawrence
  • Patent number: 11280766
    Abstract: This invention relates to encapsulated workflow reagents comprising an encapsulating material and a workflow reagent encapsulated within the encapsulating material for sample and workflow preparation prior to chromatographic, spectroscopic or other analytical systems, use thereof, and devices comprising the same.
    Type: Grant
    Filed: July 28, 2017
    Date of Patent: March 22, 2022
    Assignee: Waters Technologies Corporation
    Inventors: Kevin Daniel Wyndham, Darryl W. Brousmiche
  • Publication number: 20220080385
    Abstract: The present disclosure is directed to stationary phase materials for performing size exclusion chromatography. Embodiments of the present disclosure feature hydroxy-terminated polyethylene glycol surface modified silica particle stationary phase materials, which are optionally also methoxy-terminated polyethylene glycol surface modified.
    Type: Application
    Filed: September 16, 2021
    Publication date: March 17, 2022
    Applicant: Waters Technologies Corporation
    Inventors: Nicole L. Lawrence, Yeliz Tunc Sarisozen, Darryl W. Brousmiche, Matthew A. Lauber
  • Publication number: 20220081468
    Abstract: The present disclosure is directed to methods for performing size exclusion chromatography. Embodiments of the present disclosure feature methods for improving separations of proteinaceous analytes in size exclusion chromatography, for example, by using low concentrations of amino acids or derivatives thereof in the mobile phase.
    Type: Application
    Filed: September 16, 2021
    Publication date: March 17, 2022
    Applicant: Waters Technologies Corporation
    Inventors: Andrew Wyatt Schmudlach, Susan Rzewuski, Matthew A. Lauber, Yeliz Tunc Sarisozen, Nicole L. Lawrence, Darryl W. Brousmiche
  • Publication number: 20220080388
    Abstract: The present disclosure is directed to stationary phase materials (e.g., porous inorganic-organic hybrid particles) for performing size exclusion chromatography. Embodiments of the present disclosure feature hydroxy-terminated polyethylene glycol surface modified stationary phase materials.
    Type: Application
    Filed: September 16, 2021
    Publication date: March 17, 2022
    Applicant: Waters Technologies Corporation
    Inventors: Yeliz Tunc Sarisozen, Nicole L. Lawrence, Darryl W. Brousmiche, Andrew Wyatt Schmudlach, Matthew A. Lauber
  • Publication number: 20220017887
    Abstract: The present disclosure relates to compositions, kits and methods that may be used for removal of matrix components, including proteins and lipids, from one or more oligonucleotides.
    Type: Application
    Filed: July 14, 2021
    Publication date: January 20, 2022
    Inventors: Michael Donegan, Martin Gilar, Darryl W. Brousmiche